Relationship between perfusion pressure and renin release in the isolated rat kidney

1975 ◽  
Vol 360 (3) ◽  
pp. 255-266 ◽  
Author(s):  
H. Zschiedrich ◽  
K. G. Hofbauer ◽  
G. D. Baron ◽  
E. Hackenthal ◽  
F. Gross
Keyword(s):  
Perfusion Pressure ◽  
Rat Kidney ◽  
Renin Release ◽  
Isolated Rat Kidney ◽  
Isolated Rat

A servo-system for constant pressure perfusion of the isolated rat kidney

1986 ◽  
Vol 250 (4) ◽  
pp. F744-F748
Author(s):  
R. C. Scaduto ◽  
A. C. Schoolwerth
Keyword(s):  
Perfusion Pressure ◽  
Rat Kidney ◽  
Servo System ◽  
Technical Difficulty ◽  
Perfusion Flow ◽  
Isolated Rat Kidney ◽  
Rapid Changes ◽  
Analog Circuitry ◽  
Perfusion Flow Rate ◽  
Isolated Rat

A technical difficulty in perfusion of the isolated rat kidney has been the regulation of perfusion pressure. Since perfusion pressure is an important determinant of renal function, this parameter should be well regulated for experimental consistency. A servo-system is described that uses analog circuitry to calculate and maintain the cannula tip pressure for perfusion of the isolated kidney. The system is sufficiently responsive to maintain a constant perfusion pressure during the rapid changes in renal resistance caused by addition of angiotensin II or epinephrine to the perfusate. Analog outputs are provided for the recording of perfusion flow rate, perfusion pressure, and renal resistance.

Inhibition of Renin Secretion in the Isolated Rat Kidney by Antidiuretic Hormone

1975 ◽  
Vol 49 (1) ◽  
pp. 73-76 ◽  
Author(s):  
R. Vandongen
Keyword(s):  
Antidiuretic Hormone ◽  
Calcium Ions ◽  
Perfusion Pressure ◽  
Direct Action ◽  
Rat Kidney ◽  
Renal Perfusion ◽  
Renin Secretion ◽  
Renal Perfusion Pressure ◽  
Isolated Rat Kidney ◽  
Isolated Rat

1. The effect of antidiuretic hormone (ADH) on isoprenaline-stimulated renin secretion was examined in the isolated rat kidney perfused with modified Krebs-Ringer saline. 2. Intrarenal infusion of ADH effectively prevented stimulation of renin secretion by isoprenaline whilst increasing renal perfusion pressure. 3. The exclusion of calcium ions from the perfusion medium abolished the vasoconstrictor effect of ADH and attenuated the inhibitory effect of ADH on isoprenaline-stimulated renin secretion. However, significant suppression of renin secretion was still apparent compared with experiments where isoprenaline was infused alone. 4. These observations indicate that ADH inhibits renin secretion and that this is effected by a direct action on the kidney. Although this may be partly mediated by the rise in renal perfusion pressure, an additional direct effect of ADH on the renin-producing cell, which is dependent on the availability of calcium ions, is proposed.

scholarly journals Stimulation of renin release by hyperoncotic perfusion of the isolated rat kidney.

1982 ◽  
Vol 50 (3) ◽  
pp. 400-404 ◽  
Author(s):  
A J Cohen ◽  
K Spokes ◽  
R S Brown ◽  
J S Stoff ◽  
P Silva
Keyword(s):  
Rat Kidney ◽  
Renin Release ◽  
Isolated Rat Kidney ◽  
Isolated Rat ◽  
Stimulation Of

Effect of reduced chloride reabsorption on renin release in the isolated rat kidney

1985 ◽  
Vol 405 (1) ◽  
pp. 46-51 ◽  
Author(s):  
Stephen G. Rostand ◽  
Jack Work ◽  
Robert G. Luke
Keyword(s):  
Rat Kidney ◽  
Renin Release ◽  
Isolated Rat Kidney ◽  
Isolated Rat

Stimulatory action of parathyroid hormone on renin secretion in vitro: a study using isolated rat kidney, isolated rabbit glomeruli and superfused dispersed rat juxtaglomerular cells

1993 ◽  
Vol 84 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Christian Saussine ◽  
C. Judes ◽  
T. Massfelder ◽  
M.-J. Musso ◽  
U. Simeoni ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Rat Kidney ◽  
Renin Secretion ◽  
Renin Release ◽  
Juxtaglomerular Cells ◽  
Isolated Kidney ◽  
Stimulatory Action ◽  
Isolated Rat Kidney ◽  
Isolated Rat

1. We have previously reported that pharmacological concentrations (125 nmol/l) of parathyroid hormone may stimulate renin release in the stable recirculating and non-filtering isolated rat kidney. 2. In the present study we have attempted to extend these initial observations by examining the concentration-related response of renin release to parathyroid hormone, using the same model of isolated kidney, and determining whether the effect of parathyroid hormone on renin release can be demonstrated by more direct approaches. Thus, the effects of parathyroid hormone on renin secretion were investigated in two other renal preparations: isolated rabbit glomeruli and isolated rat juxtaglomerular cells. 3. In the isolated kidney, rat parathyroid hormone significantly stimulated renin accumulation in the perfusate in a concentration-related manner with a threshold of 1 nmol/l. 4. In both glomeruli and juxtaglomerular cells bovine [Nle8,18,Tyr34]parathyroid hormone-(1–34)amide effectively and repeatedly stimulated renin release. These results imply that there is a direct stimulatory effect of parathyroid hormone on renin release. 5. We also examined the effect of [Nle8,18, Tyr34]parathyroid hormone-(l-34)amide during extracellular calcium buffering in the glomeruli. [Nle8,18,Tyr34]parathyroid hormone-(1–34)amide was uneffective in calcium-free medium. Increasing the extracellular ionized calcium concentration to 2.5 mmol/l increased the extent of stimulation in accordance with the reported ability of parathyroid hormone to block calcium channels and relax vascular smooth muscle cells. 6. These results provide further support for the role of parathyroid hormone as a direct mediator of renin secretion; moreover, the renin-stimulating action of parathyroid hormone may be mediated through the inhibition of calcium influx.s

scholarly journals Flow-induced errors in estimating perfusion pressure of the isolated rat kidney

1982 ◽  
Vol 22 (6) ◽  
pp. 693-696 ◽  
Author(s):  
Rodger Loutzenhiser ◽  
Charles Horton ◽  
Murray Epstein
Keyword(s):  
Perfusion Pressure ◽  
Rat Kidney ◽  
Isolated Rat Kidney ◽  
Isolated Rat

Characterization of Ascorbic Acid Uptake by Isolated Rat Kidney Cells

1991 ◽  
Vol 121 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Delores M. Bowers-Komro ◽  
Donald B. McCormick
Keyword(s):  
Ascorbic Acid ◽  
Rat Kidney ◽  
Acid Uptake ◽  
Kidney Cells ◽  
Isolated Rat Kidney ◽  
Isolated Rat
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