The influence of changes in $$p_{CO_2 } $$ on the fractional packed cell volume of whole blood

1981 ◽  
Vol 392 (2) ◽  
pp. 146-151
Author(s):  
A. Luttmann ◽  
K. M�ckenhoff ◽  
H. H. Loeschcke ◽  
A. Plaas-Link
Keyword(s):  
Cell Volume ◽  
Whole Blood ◽  
Packed Cell Volume

scholarly journals Electrolyte and Haematocrit Changes in The Blood of Sheep From Foetal to Postnatal Life

1961 ◽  
Vol 14 (1) ◽  
pp. 87 ◽  
Author(s):  
JV Evans ◽  
MH Blunt
Keyword(s):  
Blood Plasma ◽  
Cell Volume ◽  
Whole Blood ◽  
Packed Cell Volume ◽  
Postnatal Life

Foetal and postnatal packed cell volume percentages and whole blood, plasma, and erythrocyte potassium and sodium concentrations have been studied in the sheep.

scholarly journals Diagnostic value of copper parameters to predict growth of suckling calves grazing native range in Argentina

2010 ◽  
Vol 30 (10) ◽  
pp. 827-832 ◽  
Author(s):  
Luis E Fazzio ◽  
Guillermo A Mattioli ◽  
Sebastian J Picco ◽  
Diana E Rosa ◽  
Leonardo Minatel ◽  
...  
Keyword(s):  
Cell Volume ◽  
Whole Blood ◽  
Packed Cell Volume ◽  
Average Daily Gain ◽  
Diagnostic Value ◽  
The Other ◽  
Gestation Period ◽  
Native Range ◽  
Daily Gain ◽  
Liver Storage

A study was conducted to evaluate the predictive diagnostic value of different copper (Cu) parameters as indicators of average daily gain (ADG) in growing calves. The effects in calves of cow Cu supplementation in the last one-third gestation period were also evaluated. Five supplementation trials, with a total of 300 calves, were carried out. Two groups of 30 calves were randomly assigned to each trial, one group was parenterally supplemented (SG) and the other was not supplemented (NSG). Trials began when calves were three-month-old and ended at weaning time. At each sampling calves were weighed and blood was taken to determine Cu concentrations in plasma, Whole Blood (WB), Red Cells (RC) and Packed Cell Volume (PCV). Liver samples from six animals of each group were taken both at the beginning and at the end of the trial. In two trials the mothers of the SG received Cu supplementation at the last one- third gestation period. Four of the five trials exhibited low ADG in the NSGs. In these groups, plasma Cu concentration decreased rapidly before low ADG was detected, which occurred with values remaining below 25µg/dl. The decrease of RC Cu concentration was considerably slow. WB showed an intermediate position. PCV in the SGs was higher than in the NSGs in all trials. Cow supplementation was insufficient to generate a liver storage able to last after calves reached the 3 months of age. These data could be useful to predict the risk of low ADG in grazing calves.

scholarly journals An investigation of the effects of intravenous administration of thiomolybdate on copper metabolism in chronic Cu-poisoned sheep

1981 ◽  
Vol 46 (3) ◽  
pp. 469-480 ◽  
Author(s):  
S. R. Gooneratne ◽  
J. McC. Howell ◽  
J. M. Gawthorne
Keyword(s):  
Cell Volume ◽  
Whole Blood ◽  
Packed Cell Volume ◽  
Copper Sulphate ◽  
Trichloroacetic Acid ◽  
Copper Metabolism ◽  
Osmotic Fragility ◽  
Soluble Form ◽  
Before And After ◽  
Copper Poisoning

1. Fourteen sheep were dosed repeatedly with a solution of copper sulphate (2 g/l) in order to induce chronic copper poisoning and four similar undosed animals acted as controls.2. Thiomolybdate (TM) was intravenously administered to all control sheep and to all except two of the test sheep. A variety of biochemical factors were studied before and after injections of TM.3. The direct-reacting Cu, whole-blood Cu and plasma Cu concentrations were elevated in animals given TM injections and at the ‘haemolytic crisis’ of untreated chronic Cu-poisoned animals. But most of the increased Cu observed on injecting TM was insoluble in trichloroacetic acid (TCA) and did not enter erythrocytes. The results indicate that uptake of Cu by erythrocytes is essential for haemolysis to occur and that for this to happen the Cu must be in a direct reacting, TCA-soluble form.4. Increased amounts of Cu were excreted in the urine at haemolysis and at the commencement of TM injections. High levels of direct-reacting Cu were found in plasma at these times.5. Marked changes were not found in caeruloplasmin activity, packed cell volume or the osmotic fragility of erythrocytes except at haemolysis. TM injections did not alter these factors in any of the sheep studied.

scholarly journals Effect of High Concentrations of Leukocytes on Whole Blood Viscosity

Blood ◽  
1971 ◽  
Vol 38 (3) ◽  
pp. 299-301 ◽  
Author(s):  
MARTIN H. STEINBERG ◽  
STANLEY E. CHARM
Keyword(s):  
Cell Volume ◽  
Blood Viscosity ◽  
Whole Blood ◽  
Packed Cell Volume ◽  
Leukocyte Count ◽  
Whole Blood Viscosity ◽  
High Concentrations ◽  
Normal Variability

Abstract Whole blood viscosity of patients with leukemic leukocytosis was compared to that of normals with a similar total packed cell volume (TPCV). Leukocytes in suspension were also studied and found to have a greater viscosity than erythrocytes. We found no relation between the magnitude of the leukocyte count and whole blood viscosity. The presence of anemia in the patients with leukemia prevented elevation of their TPCV. This, as well as the normal variability of blood viscosity obscured any increase that could have been produced by the greater viscosity of massed leukocytes.

Problems by Using Whole Blood in Heparin Measurements

1982 ◽  
Vol 47 (02) ◽  
pp. 177-181 ◽  
Author(s):  
Ingebrigt Talstad
Keyword(s):  
Cell Volume ◽  
Whole Blood ◽  
Packed Cell Volume ◽  
Partial Thromboplastin Time ◽  
High Accuracy ◽  
Activated Partial Thromboplastin Time ◽  
Clotting Time ◽  
Platelet Factor ◽  
Accuracy And Precision ◽  
Recalcification Time

SummaryProblems by using whole blood (WB) of various packed cell volume (PCV) in heparin measurements were studied. WB had to be used within 1 hr, due to influence of platelet Factor 4. The increase of Ca++ by increasing PCV, had a moderate influence by the assays studied. The recalcification time (RT) of WB was timeconsuming and had low accuracy and precision, due to different heparin response. The activated partial thromboplastin time (APTT) also had low accuracy and precision, due to different heparin response; the results were equal for WB and plasma. The thrombin clotting time (TCT) had high accuracy and precision in plasma. The TCT could also be used for WB since there was a good correlation between T20 (20 NTH U thrombin/ml) using WB, and T30 using plasma (r = 0.89, N = 61). The calcium thrombin clotting time (CaTCT) is not recommended for WB assaying.

scholarly journals Hematological parameters in largemouth bass (Micropterus salmoides) with formalin-preservation: comparison between wild tournament-caught and captive-raised fish

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6669
Author(s):  
Michelle C. Whitehead ◽  
Chelsey L. Vanetten ◽  
Yaxin Zheng ◽  
Gregory A. Lewbart
Keyword(s):  
Cell Volume ◽  
Largemouth Bass ◽  
Whole Blood ◽  
Packed Cell Volume ◽  
Wild Population ◽  
Micropterus Salmoides ◽  
Hematological Parameters ◽  
Total Leukocyte Count ◽  
Total Solids ◽  
Formalin Preservation

Background Largemouth bass (Micropterus salmoides) are an economically important freshwater fish species that have been investigated for both the short and long-term effects of stress, secondary to angling. Limited data has been published on the hematological parameters of this species and blood sample stability is a notable limitation of hematologic field studies. A relatively novel technique using 10% neutral buffered formalin preserves heparinized whole blood and maintains blood cell stability beyond one month in striped bass. The objective of this study was to evaluate the differences in hematological parameters between tournament-caught and captive-raised largemouth bass using whole blood preservation with neutral buffered formalin. Methods Two populations of largemouth bass (n = 26 wild; n = 29 captive) underwent coccygeal venipuncture to collect heparinized whole blood for packed cell volume, total solids, and manual differential. Formalin preservation of heparinized whole blood facilitated manual hemocytometer analysis. Results were compared between the populations (tournament-caught, and captive-raised) with Wilcoxon rank sum test, a Hotelling’s T2 test, and Bonferroni simultaneous 95% confidence intervals to determine significance. Results The mean packed cell volume (44.9 ± 5.4%) and total solids (7.2 ± 1.1 g/dL) were significantly higher, while the total leukocyte count (7.08 ± 1.86 × 103/µL) was significantly lower in the wild tournament-caught population of largemouth bass, as compared to the captive-raised counterparts (PCV 34.4 ± 7.2%; TS 5.2 ± 1.0 g/dL; WBC 16.43 ± 8.37 × 103/µL). The wild population demonstrated a significantly distinct leukogram characterized by a neutropenia (24.1 ± 12.7%), lymphocytosis (67.7 ± 13.0%), and monocytopenia (8.3 ± 2.9%), while the erythrocyte and thrombocyte counts were not significantly different between populations. Discussion Numerous factors have been demonstrated to influence hematologic parameters in fish including age, size, sex, temperature, environmental oxygen level, population density, and infection. The wild population endured stress during angling capture, live-well hypoxia, transport, and extended air exposures at weigh in, which may have caused a stress leukopenia as well as osmoregulatory dysfunction and subsequent hemoconcentration. Further evaluation of seasonal impact as well as increased sample size is warranted to enhance our understanding of largemouth bass hematology. Conclusion This study concluded that wild largemouth bass captured via tournament angling have higher packed cell volume and total solids, and lower total leukocyte counts, compared to captive-reared individuals. Through the completion of this study, we demonstrated the successful use of 10% neutral buffered formalin to preserve heparinized whole blood for precise hemocytometer cell counts in a new teleost species, the largemouth bass.

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