Large-scale perfusion culture process for suspended mammalian cells that uses a centrifuge with multiple settling zones

1996 ◽  
Vol 45 (4) ◽  
pp. 454-457 ◽  
Author(s):  
H. Takamatsu ◽  
K. Hamamoto ◽  
K. Ishimura ◽  
S. Yokoyama ◽  
M. Tokashiki
Keyword(s):  
Mammalian Cells ◽  
Large Scale ◽  
Perfusion Culture ◽  
Culture Process

Large-scale perfusion culture process for suspended mammalian cells that uses a centrifuge with multiple settling zones

1996 ◽  
Vol 45 (4) ◽  
pp. 454-457
Author(s):  
H. Takamatsu ◽  
K. Hamamoto ◽  
K. Ishimaru ◽  
S. Yokoyama ◽  
M. Tokashiki
Keyword(s):  
Mammalian Cells ◽  
Large Scale ◽  
Perfusion Culture ◽  
Culture Process

Potential of cell retention techniques for large-scale high-density perfusion culture of suspended mammalian cells

2003 ◽  
Vol 82 (7) ◽  
pp. 751-765 ◽  
Author(s):  
D. Voisard ◽  
F. Meuwly ◽  
P.-A. Ruffieux ◽  
G. Baer ◽  
A. Kadouri
Keyword(s):  
Mammalian Cells ◽  
Large Scale ◽  
Perfusion Culture ◽  
High Density ◽  
Cell Retention

Large Scale Suspension Perfusion Culture Process with a Compact Centrifuge for Mammalian Cell Recycle

1995 ◽  
pp. 915-919
Author(s):  
Seiichi Yokoyama ◽  
Hiroyuki Takamatsu ◽  
Kimihiko Hamamoto ◽  
Masamichi Motoki ◽  
Takami Arai ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Large Scale ◽  
Perfusion Culture ◽  
Cell Recycle ◽  
Culture Process

scholarly journals Automation of large scale transient protein expression in mammalian cells

2011 ◽  
Vol 175 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Yuguang Zhao ◽  
Benjamin Bishop ◽  
Jordan E. Clay ◽  
Weixian Lu ◽  
Margaret Jones ◽  
...  
Keyword(s):  
Protein Expression ◽  
Mammalian Cells ◽  
Large Scale ◽  
Transient Protein Expression ◽  
Expression In Mammalian Cells

Error propagation from prime variables into specific rates and metabolic fluxes for mammalian cells in perfusion culture

2009 ◽  
Vol 25 (4) ◽  
pp. 986-998 ◽  
Author(s):  
Chetan T. Goudar ◽  
Richard Biener ◽  
Konstantin B. Konstantinov ◽  
James M. Piret
Keyword(s):  
Error Propagation ◽  
Mammalian Cells ◽  
Perfusion Culture ◽  
Metabolic Fluxes

scholarly journals Transgenic goats producing an improved version of cetuximab in milk

2020 ◽  
Author(s):  
Götz Laible ◽  
Sally Cole ◽  
Brigid Brophy ◽  
Paul Maclean ◽  
Li How Chen ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Large Scale ◽  
Growth Factor Receptor ◽  
Cost Effective ◽  
Scale Production ◽  
Cancer Treatments ◽  
Large Scale Production ◽  
Immunogenic Epitope ◽  
Dependent Cell ◽  
Transgenic Goats

ABSTRACTTherapeutic monoclonal antibodies (mAbs) represent one of the most important classes of pharmaceutical proteins to treat human diseases. Most are produced in cultured mammalian cells which is expensive, limiting their availability. Goats, striking a good balance between a relatively short generation time and copious milk yield, present an alternative platform for the cost-effective, flexible, large-scale production of therapeutic mAbs. Here, we focused on cetuximab, a mAb against epidermal growth factor receptor, that is commercially produced under the brand name Erbitux and approved for anti-cancer treatments. We generated several transgenic goat lines that produce cetuximab in their milk. Two lines were selected for detailed characterization. Both showed stable genotypes and cetuximab production levels of up to 10g/L. The mAb could be readily purified and showed improved characteristics compared to Erbitux. The goat-produced cetuximab (gCetuximab) lacked a highly immunogenic epitope that is part of Erbitux. Moreover, it showed enhanced binding to CD16 and increased antibody-dependent cell-dependent cytotoxicity compared to Erbitux. This indicates that these goats produce an improved cetuximab version with the potential for enhanced effectiveness and better safety profile compared to treatments with Erbitux. In addition, our study validates transgenic goats as an excellent platform for large-scale production of therapeutic mAbs.

CO2 in large-scale and high-density CHO cell perfusion culture

1996 ◽  
Vol 22 (1-3) ◽  
pp. 65-78 ◽  
Author(s):  
David R. Gray ◽  
Su Chen ◽  
William Howarth ◽  
Duane Inlow ◽  
Brian L. Maiorella
Keyword(s):  
Large Scale ◽  
Perfusion Culture ◽  
High Density ◽  
Cho Cell

Perfusion culture apparatus for suspended mammalian cells

1993 ◽  
Vol 13 (3) ◽  
pp. 149-159 ◽  
Author(s):  
Michiyuki Tokashiki ◽  
Hiroyuki Takamatsu
Keyword(s):  
Mammalian Cells ◽  
Perfusion Culture

Large–Scale Production of Proteins from Mammalian Cells

1988 ◽  
Vol 6 (5) ◽  
pp. 518-523 ◽  
Author(s):  
Malcolm Rhodes ◽  
John Birch
Keyword(s):  
Mammalian Cells ◽  
Large Scale ◽  
Scale Production ◽  
Large Scale Production
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