Potential of cell retention techniques for large-scale high-density perfusion culture of suspended mammalian cells

2003 ◽  
Vol 82 (7) ◽  
pp. 751-765 ◽  
Author(s):  
D. Voisard ◽  
F. Meuwly ◽  
P.-A. Ruffieux ◽  
G. Baer ◽  
A. Kadouri
Keyword(s):  
Mammalian Cells ◽  
Large Scale ◽  
Perfusion Culture ◽  
High Density ◽  
Cell Retention

CO2 in large-scale and high-density CHO cell perfusion culture

1996 ◽  
Vol 22 (1-3) ◽  
pp. 65-78 ◽  
Author(s):  
David R. Gray ◽  
Su Chen ◽  
William Howarth ◽  
Duane Inlow ◽  
Brian L. Maiorella
Keyword(s):  
Large Scale ◽  
Perfusion Culture ◽  
High Density ◽  
Cho Cell

Solution to the high Dissolved CO2 Problem in High-Density Perfusion Culture of Mammalian Cells

2001 ◽  
pp. 399-402 ◽  
Author(s):  
R. Matanguihan ◽  
E. Sajan ◽  
M. Zachariou ◽  
C. Olson ◽  
Jim Michaels ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Perfusion Culture ◽  
High Density

Large-scale perfusion culture process for suspended mammalian cells that uses a centrifuge with multiple settling zones

1996 ◽  
Vol 45 (4) ◽  
pp. 454-457
Author(s):  
H. Takamatsu ◽  
K. Hamamoto ◽  
K. Ishimaru ◽  
S. Yokoyama ◽  
M. Tokashiki
Keyword(s):  
Mammalian Cells ◽  
Large Scale ◽  
Perfusion Culture ◽  
Culture Process

Large-scale perfusion culture process for suspended mammalian cells that uses a centrifuge with multiple settling zones

1996 ◽  
Vol 45 (4) ◽  
pp. 454-457 ◽  
Author(s):  
H. Takamatsu ◽  
K. Hamamoto ◽  
K. Ishimura ◽  
S. Yokoyama ◽  
M. Tokashiki
Keyword(s):  
Mammalian Cells ◽  
Large Scale ◽  
Perfusion Culture ◽  
Culture Process

scholarly journals Automation of large scale transient protein expression in mammalian cells

2011 ◽  
Vol 175 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Yuguang Zhao ◽  
Benjamin Bishop ◽  
Jordan E. Clay ◽  
Weixian Lu ◽  
Margaret Jones ◽  
...  
Keyword(s):  
Protein Expression ◽  
Mammalian Cells ◽  
Large Scale ◽  
Transient Protein Expression ◽  
Expression In Mammalian Cells

Large‐scale high‐density culture of hepatocytes in a liver microsystem with mimicked sinusoid blood flow

2018 ◽  
Vol 12 (12) ◽  
pp. 2266-2276
Author(s):  
Jing Liu ◽  
Chengpan Li ◽  
Shaohui Cheng ◽  
Shengnan Ya ◽  
Dayong Gao ◽  
...  
Keyword(s):  
Blood Flow ◽  
Large Scale ◽  
High Density ◽  
High Density Culture ◽  
Culture Of Hepatocytes

Error propagation from prime variables into specific rates and metabolic fluxes for mammalian cells in perfusion culture

2009 ◽  
Vol 25 (4) ◽  
pp. 986-998 ◽  
Author(s):  
Chetan T. Goudar ◽  
Richard Biener ◽  
Konstantin B. Konstantinov ◽  
James M. Piret
Keyword(s):  
Error Propagation ◽  
Mammalian Cells ◽  
Perfusion Culture ◽  
Metabolic Fluxes

scholarly journals YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3069
Author(s):  
Zheng Liu ◽  
Ling Lin ◽  
Haozhe Zhu ◽  
Zhongyuan Wu ◽  
Xi Ding ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Large Scale ◽  
High Density ◽  
Meat Production ◽  
Density Condition ◽  
High Density Culture ◽  
Differentiation Potential ◽  
Muscle Stem Cells ◽  
Cultured Meat

Muscle stem cells (MuSCs) isolated ex vivo are essential original cells to produce cultured meat. Currently, one of the main obstacles for cultured meat production derives from the limited capacity of large-scale amplification of MuSCs, especially under high-density culture condition. Here, we show that at higher cell densities, proliferation and differentiation capacities of porcine MuSCs are impaired. We investigate the roles of Hippo-YAP signaling, which is important regulators in response to cell contact inhibition. Interestingly, abundant but not functional YAP proteins are accumulated in MuSCs seeded at high density. When treated with lysophosphatidic acid (LPA), the activator of YAP, porcine MuSCs exhibit increased proliferation and elevated differentiation potential compared with control cells. Moreover, constitutively active YAP with deactivated phosphorylation sites, but not intact YAP, promotes cell proliferation and stemness maintenance of MuSCs. Together, we reveal a potential molecular target that enables massive MuSCs expansion for large-scale cultured meat production under high-density condition.

scholarly journals Enhancing Form-Based Code: A parametric approach to urban volumetric morphology in high density cities

2021 ◽  
Author(s):  
◽  
Yingyi Zhang
Keyword(s):  
Hong Kong ◽  
Large Scale ◽  
Positive Impact ◽  
High Density ◽  
Original Form ◽  
Human Society ◽  
Parametric Modelling ◽  
Physical Form ◽  
Urban Regulation ◽  
Modelling System

<p>Parametric tools have been broadly implemented in Architecture, Engineering and Construction (AEC) industry. Recently, an increasing volume of research finds that parametric tools also have the capability to facilitate large-scale planning and urban design. Much of this research, however, focuses on parametric representation or environment simulation. There is insufficient research about using parametric tools to enhance urban regulation. Parametric tools can provide smart design procedures by integrating strategies, solutions and expressions in one system. They may allow alternative approaches to urban regulation that conventional tools do not process.  This research aims to create a parametric modelling system to aid urban regulation. The system offers a visualised coding interface to manipulate parameters and achieve interactive performance feedback at the early stage of urban regulation. Form-Based Code uses the modelling system in this research. It generates a specific morphology by controlling physical form with less focus on land use. With the rise of New Urbanism, Form-Based Code has been used in various American regulation projects. This research extends the application of Form-Based Code, adopting it for urban-peripheral environments outside of the USA. High-density cities where provide the volumetric morphology context is important for this work. Tsim Sha Tsui area of Hong Kong works as an experimental site.  The feasibility of parametric urban regulation is examined by developing a parametric modelling system for Form-Based Code in Hong Kong. Understanding the site’s form characteristics, the transect matrix of Form-Based Code is expanded by incorporating multi-layered zone types and regulating plans. Embedding the zones into parametric modelling software Rhinoceros 3D and Grasshopper 3D, a regenerative prototype works to create real-time scenarios responding to parameters, rules and geometry constraints. The results of parametric urban regulation are evaluated by both Form-Based Code standards and local urban regulation standards to assess its feasibility in context.  This research demonstrates that the parametric modelling system for Form-Based Code has both technological and implemental potential to work as an alternative approach to urban regulation, especially in complex developments. Form complexity is a reflection of sophisticated human-society systems and the sequential evolution of a dynamic morphology. Form-Based Code is enhanced by the parametric modelling system to describe and regulate form complexity in a logical manner. Additionally, although parametric Form-Based Code processing is based on the original Form-Based Code, it is not limited to that. Describing urban regulation with visualised models bridges specialists and the public in community demonstrations and code assembling. The parametric modelling system has a positive impact on resolving challenges, predicting outcomes, and applying urban regulation innovation to the volumetric morphology of high-density cities in Asia.</p>

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