Genetic variants of placental alkaline phosphatase as detected by a monoclonal antibody

1982 ◽  
Vol 60 (2) ◽  
pp. 145-149 ◽  
Author(s):  
Jos� Luis Mill�n ◽  
Gunhild Beckman ◽  
Annika Jeppsson ◽  
Torgny Stigbrand
Keyword(s):  
Monoclonal Antibody ◽  
Alkaline Phosphatase ◽  
Genetic Variants ◽  
Placental Alkaline Phosphatase

Use of monoclonal antibodies to detect human placental alkaline phosphatase.

1983 ◽  
Vol 29 (1) ◽  
pp. 115-119 ◽  
Author(s):  
G De Groote ◽  
P De Waele ◽  
A Van de Voorde ◽  
M De Broe ◽  
W Fiers
Keyword(s):  
Monoclonal Antibody ◽  
Alkaline Phosphatase ◽  
Monoclonal Antibodies ◽  
Solid Phase ◽  
Enzymatic Reactions ◽  
Placental Alkaline Phosphatase ◽  
Human Placental Alkaline Phosphatase ◽  
Human Sera ◽  
Starch Gel ◽  
Alkaline Phosphatase Isoenzyme

Abstract Convenient, sensitive, and specific solid-phase immunoassays involving monoclonal antibody are described for the determination of human placental alkaline phosphatase (hPLAP). An endogenous enzyme immunoassay combined the specificity of the immunological and the enzymatic reactions. Alternatively, a solid-phase "sandwich" radioimmunoassay involving immobilized polyclonal rabbit anti-hPLAP in combination with iodinated monoclonal antibody provided some additional advantages. Both tests can be used to detect hPLAP from various sources, e.g., in human sera during pregnancy or as a tumor marker. The radioimmunoassay detected an increase in hPLAP at nine weeks of gestation. We discuss the use of monoclonal antibodies for the differentiation of different alkaline phosphatase isoenzyme types by electrophoresis on starch gel.

A Potential Use of a Monoclonal Antibody to Placental Alkaline Phosphatase (PLAP) to Detect Lymph Node Metastases of Seminoma

1996 ◽  
Vol 155 (1) ◽  
pp. 337-341 ◽  
Author(s):  
Kiyoshi Koshida ◽  
Tadao Uchibayashi ◽  
Hajime Yamamoto ◽  
Kunihiko Yokoyama ◽  
Kazuyuki Hirano
Keyword(s):  
Monoclonal Antibody ◽  
Alkaline Phosphatase ◽  
Lymph Node ◽  
Lymph Node Metastases ◽  
Placental Alkaline Phosphatase ◽  
Node Metastases ◽  
Potential Use

Immunolocalization of anti-placental alkaline phosphatase monoclonal antibody in mice with testicular tumors and lymph node metastasis

1998 ◽  
Vol 26 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Kiyoshi Koshida ◽  
Kunihiko Yokoyama ◽  
Tetsuya Imao ◽  
Hiroyuki Konaka ◽  
Kazuyuki Hirano ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Alkaline Phosphatase ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Placental Alkaline Phosphatase ◽  
Testicular Tumors ◽  
Node Metastasis
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