Immunolocalization of anti-placental alkaline phosphatase monoclonal antibody in mice with testicular tumors and lymph node metastasis

1998 ◽  
Vol 26 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Kiyoshi Koshida ◽  
Kunihiko Yokoyama ◽  
Tetsuya Imao ◽  
Hiroyuki Konaka ◽  
Kazuyuki Hirano ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Alkaline Phosphatase ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Placental Alkaline Phosphatase ◽  
Testicular Tumors ◽  
Node Metastasis

A Potential Use of a Monoclonal Antibody to Placental Alkaline Phosphatase (PLAP) to Detect Lymph Node Metastases of Seminoma

1996 ◽  
Vol 155 (1) ◽  
pp. 337-341 ◽  
Author(s):  
Kiyoshi Koshida ◽  
Tadao Uchibayashi ◽  
Hajime Yamamoto ◽  
Kunihiko Yokoyama ◽  
Kazuyuki Hirano
Keyword(s):  
Monoclonal Antibody ◽  
Alkaline Phosphatase ◽  
Lymph Node ◽  
Lymph Node Metastases ◽  
Placental Alkaline Phosphatase ◽  
Node Metastases ◽  
Potential Use

scholarly journals The Lymphatic Infiltration Identified by D2-40 Monoclonal Antibody Predicts Lymph Node Metastasis in Submucosal Invasive Colorectal Cancer

Pathobiology ◽  
2007 ◽  
Vol 74 (6) ◽  
pp. 328-335 ◽  
Author(s):  
Ken Kawaura ◽  
Satoshi Fujii ◽  
Yukinori Murata ◽  
Takahiro Hasebe ◽  
Genichiro Ishii ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Monoclonal Antibody ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Node Metastasis

Mismatch Repair Gene Expression In Testicular Germ Cell Tumors And Retroperitoneal Lymph Node Metastasis

2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S74-S75
Author(s):  
M Youssef ◽  
M Rodriguez Pena ◽  
S Canete-Portillo ◽  
S Al Diffalha ◽  
C Magi-Galluzzi
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Staining Pattern ◽  
Germ Cell Tumors ◽  
Nuclear Staining ◽  
Testicular Germ Cell Tumors ◽  
Retroperitoneal Lymph Node ◽  
Testicular Tumors ◽  
Testicular Germ Cell ◽  
Node Metastasis

Abstract Introduction/Objective Testicular germ cell tumors (TCGT) with somatic mismatch repair pathway defects have shown to have worse prognosis and response to therapy. Immunohistochemical staining pattern of MMR panel was classified in a previous study as low (absent or minimal) and high staining (moderate or high staining), with the low staining pattern suggestive of poor prognosis and high risk of recurrence. Herein, we studied the MMR staining pattern in TGCT and lymph node metastasis. Methods 21 TGCT were included in the study: 10 primary testicular tumors and 11 unrelated retroperitoneal lymph node metastasis. All cases were analyzed for MMR protein expression by immunohistochemistry. Clinical, histopathological and follow-up was obtained in all cases. Retained MLH1, MSH2, MSH6, and PMS2 was defined as nuclear staining. Loss of expression was defined as absence of nuclear staining within tumor cells with retained expression in internal control cells, including benign testicular tissue, stromal cells, and infiltrating lymphocytes. Results All primary testicular tumors were mixed GCT: embryonal carcinomas was present in 5, yolk sac tumor in 3 and teratoma in 6 cases. One (10%) of the primary tumors showed focal loss of MLH1 and PMS2 expression in a focus of embryonal carcinoma. All lymph node metastases consisted of teratoma. Seven (64%) metastatic cases showed scattered areas of focal MLH1 loss, 5 (45%) of which showed also focal loss of PMS2. High staining for MSH2 and MSH6 was detected in all 21 cases. Conclusion In our small cohort, focal loss of MLH1 and PMS2 was detected in 10% of primary TGCT, compared to focal loss of MLH1 and PMS2 is 64% and 45%, respectively, of metastatic tumors, suggesting a possible relationship between heterogeneous MLH1 and PMS2 expression and retroperitoneal lymph node metastasis. The current cohort will be expanded to include additional cases.

Sign in / Sign up

Export Citation Format

Share Document