In vitro synthesis of immunoglobulins and IgM-rheumatoid factor by blood mononuclear cells of patients with rheumatoid arthritis

1982 ◽  
Vol 2 (2) ◽  
pp. 59-66 ◽  
Author(s):  
N. Olsen ◽  
M. Ziff ◽  
H. E. Jasin
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Mononuclear Cells ◽  
In Vitro Synthesis ◽  
Igm Rheumatoid Factor ◽  
Blood Mononuclear Cells

scholarly journals In vitro effects of Epstein-Barr virus on peripheral blood mononuclear cells from patients with rheumatoid arthritis and normal subjects.

1978 ◽  
Vol 148 (5) ◽  
pp. 1429-1434 ◽  
Author(s):  
L Slaughter ◽  
D A Carson ◽  
F C Jensen ◽  
T L Holbrook ◽  
J H Vaughan
Keyword(s):  
Rheumatoid Arthritis ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Epstein Barr Virus ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Barr Virus ◽  
Blood Mononuclear Cells ◽  
Epstein Barr

Peripheral blood mononuclear cells from 10 patients with rheumatoid arthritis and 9 control subjects were cultured in vitro for 30 days with and without infection by Epstein-Barr virus. All cultures showed polyclonal stimulation of B cells as indicated by rising levels of IgM in the culture supernates, reaching maximal at 18-24 days, and with no quantitative or kinetic difference between the RA and control cells. IgM anti-IgG was also produced in both groups and maximally at 18-24 days, but in greater quantity by the RA lymphocytes. The anti-IgG made by the RA lymphocytes was more easily absorbed by solid phase IgG than was the anti-IgG made by the normal lymphocytes and thus was judged to be of higher affinity. RA lymphocytes uninfected with EBV had higher transformation scores than did the normal controls and developed spontaneously into permanent cell lines in six instances.

IgM rheumatoid factor amplifies the inflammatory response of macrophages induced by the rheumatoid arthritis-specific immune complexes containing anticitrullinated protein antibodies

2014 ◽  
Vol 74 (7) ◽  
pp. 1425-1431 ◽  
Author(s):  
Lætitia Laurent ◽  
Florence Anquetil ◽  
Cyril Clavel ◽  
Ndiémé Ndongo-Thiam ◽  
Géraldine Offer ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Immune Complexes ◽  
Cytokine Response ◽  
Igm Rheumatoid Factor ◽  
Tnf Α ◽  
Vitro Model ◽  
Synovial Membranes ◽  
Anticitrullinated Protein Antibodies

ObjectivesAnticitrullinated protein antibodies (ACPA) are specifically associated with rheumatoid arthritis (RA) and produced in inflamed synovial membranes where citrullinated fibrin, their antigenic target, is abundant. We showed that immune complexes containing IgG ACPA (ACPA-IC) induce FcγR-mediated tumour necrosis factor (TNF)-α secretion in macrophages. Since IgM rheumatoid factor (RF), an autoantibody directed to the Fc fragment of IgG, is also produced and concentrated in the rheumatoid synovial tissue, we evaluated its influence on macrophage stimulation by ACPA-IC.MethodsWith monocyte-derived macrophages from more than 40 healthy individuals and different human IgM cryoglobulins with RF activity, using a previously developed human in vitro model, we evaluated the effect of the incorporation of IgM RF into ACPA-IC.ResultsIgM RF induced an important amplification of the TNF-α secretion. This effect was not observed in monocytes and depended on an increase in the number of IgG-engaged FcγR. It extended to the secretion of interleukin (IL)-1β and IL-6, was paralleled by IL-8 secretion and was not associated with overwhelming secretion of IL-10 or IL-1Ra. Moreover, the RF-induced increased proinflammatory bioactivity of the cytokine response to ACPA-IC was confirmed by an enhanced, not entirely TNF-dependent, capacity of the secreted cytokine cocktail to prompt IL-6 secretion by RA synoviocytes.ConclusionsBy showing that it can greatly enhance the proinflammatory cytokine response induced in macrophages by the RA-specific ACPA-IC, these results highlight a previously undescribed, FcγR-dependent strong proinflammatory potential of IgM RF. They clarify the pathophysiological link between the presence of ACPA and IgM RF, and RA severity.

scholarly journals Human alpha-interferon enhances in vitro igm rheumatoid factor synthesis by lymphocytes from normal subjects and rheumatoid arthritis patients

1983 ◽  
Vol 26 (9) ◽  
pp. 1091-1097 ◽  
Author(s):  
Martin A. Rodriguez ◽  
William A. Prinz ◽  
Wilmer L. Sibbitt ◽  
Arthur D. Bankhurst ◽  
Ralph C. Williams
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Normal Subjects ◽  
Alpha Interferon ◽  
Igm Rheumatoid Factor

Enhanced in vitro IgM rheumatoid factor synthesis in seropositive rheumatoid arthritis families

1987 ◽  
Vol 7 (2) ◽  
pp. 71-75 ◽  
Author(s):  
G. S. Alarcón ◽  
B. O. Barger ◽  
R. C. P. Go ◽  
R. T. Acton ◽  
R. E. Schrohenloher ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Igm Rheumatoid Factor ◽  
Seropositive Rheumatoid Arthritis

In vitro synthesis of IgM and IgM rheumatoid factor in seronegative arthritides

1984 ◽  
Vol 4 (2) ◽  
pp. 49-53 ◽  
Author(s):  
G. S. Alarcón ◽  
W. J. Koopman ◽  
R. E. Schrohenloher
Keyword(s):  
Rheumatoid Factor ◽  
In Vitro Synthesis ◽  
Igm Rheumatoid Factor

In vitro IgM and IgM rheumatoid factor production and response to remittive agents in rheumatoid arthritis

1985 ◽  
Vol 28 (3) ◽  
pp. 356-357 ◽  
Author(s):  
Graciela S. Alarcón ◽  
William J. Koopman ◽  
Ralph E. Schrohenloher
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Igm Rheumatoid Factor ◽  
Factor Production
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