Enhanced in vitro IgM rheumatoid factor synthesis in seropositive rheumatoid arthritis families

1987 ◽  
Vol 7 (2) ◽  
pp. 71-75 ◽  
Author(s):  
G. S. Alarcón ◽  
B. O. Barger ◽  
R. C. P. Go ◽  
R. T. Acton ◽  
R. E. Schrohenloher ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Igm Rheumatoid Factor ◽  
Seropositive Rheumatoid Arthritis

IgM rheumatoid factor amplifies the inflammatory response of macrophages induced by the rheumatoid arthritis-specific immune complexes containing anticitrullinated protein antibodies

2014 ◽  
Vol 74 (7) ◽  
pp. 1425-1431 ◽  
Author(s):  
Lætitia Laurent ◽  
Florence Anquetil ◽  
Cyril Clavel ◽  
Ndiémé Ndongo-Thiam ◽  
Géraldine Offer ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Immune Complexes ◽  
Cytokine Response ◽  
Igm Rheumatoid Factor ◽  
Tnf Α ◽  
Vitro Model ◽  
Synovial Membranes ◽  
Anticitrullinated Protein Antibodies

ObjectivesAnticitrullinated protein antibodies (ACPA) are specifically associated with rheumatoid arthritis (RA) and produced in inflamed synovial membranes where citrullinated fibrin, their antigenic target, is abundant. We showed that immune complexes containing IgG ACPA (ACPA-IC) induce FcγR-mediated tumour necrosis factor (TNF)-α secretion in macrophages. Since IgM rheumatoid factor (RF), an autoantibody directed to the Fc fragment of IgG, is also produced and concentrated in the rheumatoid synovial tissue, we evaluated its influence on macrophage stimulation by ACPA-IC.MethodsWith monocyte-derived macrophages from more than 40 healthy individuals and different human IgM cryoglobulins with RF activity, using a previously developed human in vitro model, we evaluated the effect of the incorporation of IgM RF into ACPA-IC.ResultsIgM RF induced an important amplification of the TNF-α secretion. This effect was not observed in monocytes and depended on an increase in the number of IgG-engaged FcγR. It extended to the secretion of interleukin (IL)-1β and IL-6, was paralleled by IL-8 secretion and was not associated with overwhelming secretion of IL-10 or IL-1Ra. Moreover, the RF-induced increased proinflammatory bioactivity of the cytokine response to ACPA-IC was confirmed by an enhanced, not entirely TNF-dependent, capacity of the secreted cytokine cocktail to prompt IL-6 secretion by RA synoviocytes.ConclusionsBy showing that it can greatly enhance the proinflammatory cytokine response induced in macrophages by the RA-specific ACPA-IC, these results highlight a previously undescribed, FcγR-dependent strong proinflammatory potential of IgM RF. They clarify the pathophysiological link between the presence of ACPA and IgM RF, and RA severity.

scholarly journals Human alpha-interferon enhances in vitro igm rheumatoid factor synthesis by lymphocytes from normal subjects and rheumatoid arthritis patients

1983 ◽  
Vol 26 (9) ◽  
pp. 1091-1097 ◽  
Author(s):  
Martin A. Rodriguez ◽  
William A. Prinz ◽  
Wilmer L. Sibbitt ◽  
Arthur D. Bankhurst ◽  
Ralph C. Williams
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Normal Subjects ◽  
Alpha Interferon ◽  
Igm Rheumatoid Factor

In vitro IgM and IgM rheumatoid factor production and response to remittive agents in rheumatoid arthritis

1985 ◽  
Vol 28 (3) ◽  
pp. 356-357 ◽  
Author(s):  
Graciela S. Alarcón ◽  
William J. Koopman ◽  
Ralph E. Schrohenloher
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Igm Rheumatoid Factor ◽  
Factor Production

scholarly journals A spontaneous rheumatoid arthritis-like disease in MRL/l mice.

1982 ◽  
Vol 155 (6) ◽  
pp. 1690-1701 ◽  
Author(s):  
L Hang ◽  
A N Theofilopoulos ◽  
F J Dixon
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Joint Inflammation ◽  
Close Correlation ◽  
Human Rheumatoid Arthritis ◽  
Igm Rheumatoid Factor ◽  
Joint Pathology ◽  
Old Females

MRL/l mice spontaneously develop an arthritis very similar in many respects to human rheumatoid arthritis. A detailed morphologic and serologic analysis of this disease revealed the following: (a) a 75% incidence of synovial and periarticular inflammation, very similar to human rheumatoid arthritis, in 5-6 mo-old females, (b) close associations between presence of joint inflammation and subsynovial and/or periarticular vasculitis, and (c) a close correlation between presence of circulating IgM rheumatoid factor (RF) and demonstrable synovial and/or joint pathology, i.e., 95% of mice with significant levels of IgMRF had synovitis and/or arthritis.

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