Fluorescence polarization of stretched polytene chromosomes stained with acridine orange

1978 ◽  
Vol 4 (2) ◽  
pp. 97-110 ◽  
Author(s):  
A. D. Gruzdev ◽  
G. P. Kishchenko
Keyword(s):  
Acridine Orange ◽  
Fluorescence Polarization ◽  
Polytene Chromosomes

scholarly journals THERMAL DEPOLARIZATION OF FLUORESCENCE FROM POLYTENE CHROMOSOMES STAINED WITH ACRIDINE ORANGE

1967 ◽  
Vol 33 (3) ◽  
pp. 597-604 ◽  
Author(s):  
James W. MacInnes ◽  
Robert B. Uretz
Keyword(s):  
Acridine Orange ◽  
Rotational Diffusion ◽  
Polytene Chromosomes ◽  
Degree Of Polarization ◽  
Fourth Chromosome ◽  
Thermal Change ◽  
Low Concentrations ◽  
Thermal Denaturation Temperature ◽  
Dna Thermal Denaturation ◽  
Increasing Temperature

The degree of polarization of fluorescence from stretched Chironomus thummi polytene chromosomes, stained with low concentrations of acridine orange (AO), decreases with increasing temperature. The "half temperature" of this decrease (T½R) is lower than the expected DNA thermal denaturation temperature (Tm) by about 20°C. T½R is lowered as histone is removed from chromosomes. Balbiani ring regions of the fourth chromosome have T½R's much lower than other regions, and nearly as low as chromosomes which had been extensively pretreated with trypsin to remove histone and other proteins. Measurements of the thermal change in the rotational diffusion rate of AO in solution with DNA indicate that the temperature at which the DNA-AO bonding changes from a "rigid" to a "loose" mode varies with the GC percentage of the DNA, and in the same fashion as Tm, although 20°C lower.

Metal mutagens and carcinogens effectively displace acridine orange from DNA as measured by fluorescence polarization

1981 ◽  
Vol 3 (5) ◽  
pp. 545-553 ◽  
Author(s):  
Carol L. Richardson ◽  
James Verna ◽  
Gail E. Schulman ◽  
Kevin Shipp ◽  
Ava D. Grant
Keyword(s):  
Acridine Orange ◽  
Fluorescence Polarization

Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

1990 ◽  
Vol 48 (3) ◽  
pp. 168-169
Author(s):  
M. H. Chestnut ◽  
C. E. Catrenich
Keyword(s):  
Acridine Orange ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ulcer Disease ◽  
Gastroduodenal Disease ◽  
H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

Sign in / Sign up

Export Citation Format

Share Document