Metal mutagens and carcinogens effectively displace acridine orange from DNA as measured by fluorescence polarization

Carol L. Richardson; James Verna; Gail E. Schulman; Kevin Shipp; Ava D. Grant

doi:10.1002/em.2860030506

Metal mutagens and carcinogens effectively displace acridine orange from DNA as measured by fluorescence polarization

Environmental Mutagenesis ◽

10.1002/em.2860030506 ◽

1981 ◽

Vol 3 (5) ◽

pp. 545-553 ◽

Cited By ~ 12

Author(s):

Carol L. Richardson ◽

James Verna ◽

Gail E. Schulman ◽

Kevin Shipp ◽

Ava D. Grant

Keyword(s):

Acridine Orange ◽

Fluorescence Polarization

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Fluorescence polarization of stretched polytene chromosomes stained with acridine orange

Biophysics of Structure and Mechanism ◽

10.1007/bf00539224 ◽

1978 ◽

Vol 4 (2) ◽

pp. 97-110 ◽

Cited By ~ 4

Author(s):

A. D. Gruzdev ◽

G. P. Kishchenko

Keyword(s):

Acridine Orange ◽

Fluorescence Polarization ◽

Polytene Chromosomes

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Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100158388 ◽

1990 ◽

Vol 48 (3) ◽

pp. 168-169

Author(s):

M. H. Chestnut ◽

C. E. Catrenich

Keyword(s):

Acridine Orange ◽

Mammalian Cells ◽

Laser Scanning ◽

Laser Scanning Microscopy ◽

Confocal Laser ◽

Ulcer Disease ◽

Gastroduodenal Disease ◽

H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

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