Prostaglandin release from and formation in perfused frog intestine

1968 ◽  
Vol 259 (2) ◽  
pp. 153-154 ◽  
Author(s):  
J. Bartels ◽  
W. Vogt ◽  
G. Wille
1992 ◽  
Vol 46 (5) ◽  
pp. 829-845 ◽  
Author(s):  
Mitsunori Kasamo ◽  
Monica Brandt ◽  
Mutsuo Ishikawa ◽  
Tetsuya Shimizu ◽  
Michael J.K. Harper

2012 ◽  
Vol 188 (10) ◽  
pp. 5132-5141 ◽  
Author(s):  
Soo Jung Park ◽  
Jee Hoon Lee ◽  
Hee Young Kim ◽  
Youn Hee Choi ◽  
Jung Sup Park ◽  
...  

1973 ◽  
Vol 51 (1) ◽  
pp. 22-28
Author(s):  
Joël de la Noüe ◽  
André Gagnon

In order to calculate the intracellular concentration of accumulated L-alanine, the extracellular space (inulin-14C) of frog intestine was measured. To check the validity of the technique, frog liver and gastrocnemius were used too. By scraping proximal portions of intestine, the inulin space was found to be similar (around 20% of total tissue water) in both the muscle layer and the mucosa. The mucosal epithelium is an imperfect barrier to inulin while the serosa is very permeable. These results suggest that the interstitial solute concentration is best approximated by equating it to that of the serosal solution. The in vitro inulin space, compared to the in vivo one, increases with time, as does the cellular hydration. The data obtained from measurements of extracellular space and from L-alanine uptake show that the intracellular amino acid is in a free state.


1978 ◽  
Vol 34 (1) ◽  
pp. 159-171
Author(s):  
A. Gyevai ◽  
P.J. Chapple ◽  
W.H. Douglas

Hypothalamic aggregate cultures were developed from hypothalami taken from rat embryos at 17–19 days gestation. The aggregate cultures exhibited a prominent morphological differentiation during 3–4 weeks in culture. The fine structure of the synapses formed in the aggregates resembled synapses in tha adult animal. During synaptogenesis the aggregates spontaneously release prostaglandin E2 (PGE2). The amount of PGE2 released in the media was reversed upon the morphological differentiation of the hypothalamic cultures. Media containing a higher PGE2 concentration increased the extracellular prolactin accumulation in monolayer cultures developed from adult rat hypophysis.


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