A simple fixation procedure for immunofluorescent detection of different cytoskeletal components within the same cell

1989 ◽  
Vol 91 (1) ◽  
pp. 81-88 ◽  
Author(s):  
U. Vielkind ◽  
S. H. Swierenga
Keyword(s):  
Fixation Procedure ◽  
Simple Fixation

scholarly journals Association of a protease with polytene chromosomes of Drosophila melanogaster.

1980 ◽  
Vol 87 (2) ◽  
pp. 415-419 ◽  
Author(s):  
J Cavagnaro ◽  
D A Pierce ◽  
J C Lucchesi ◽  
C B Chae
Keyword(s):  
Acetic Acid ◽  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Protease Inhibitors ◽  
Polytene Chromosomes ◽  
Fixation Procedure ◽  
Apparent Effect ◽  
Chloromethyl Ketone ◽  
Diisopropyl Fluorophosphate ◽  
Uniform Labeling

Incubation of Drosophila salivary glands with radioactive diisopropyl fluorophosphate results in the uniform labeling of polytene chromosomes. Extensive labeling is seen only when chromosome squashes are prepared by a formaldehyde fixation procedure and not by standard acetic acid techniques. The labeling is inhibited in the presence of tosylphenylalanine chloromethyl ketone and phenylmethane sulfonylfluoride but not by tosyllysine chloromethyl ketone, suggesting that a chymotrypsin-like serine protease is associated with the chromosomes. Protease inhibitors show no apparent effect on heat-shock specific puffing.

Total hip replacement: modes of failure

2011 ◽  
Author(s):  
Rob Pollock
Keyword(s):  
Aseptic Loosening ◽  
Success Rates ◽  
Total Hip ◽  
Reactive Protein ◽  
Modes Of Failure ◽  
Total Hip Replacements ◽  
Hip Replacements ◽  
Simple Fixation

♦ Total hip replacements (THRs) may fail in various ways. They may become infected, they may be subject to aseptic loosening, they may dislocate, or a periprosthetic fracture may occur. The patient with a failed THR must be thoroughly assessed before treatment is contemplated♦ Infection may be acute or chronic. Assessment involves clinical assessment, plain radiographs, blood tests (C-reactive protein and erythrocyte sedimentation rate), hip aspiration, and, sometimes, nuclear medicine. The acutely infected hip may be treated with one-stage revision. This involves thorough lavage, debridement, and exchange of all modular components as well as long-term antibiotic therapy. The gold standard of treatment for a chronically infected THR is a two-stage revision. Success rates of 80–90% can be expected♦ Aseptic loosening typically occurs at the cement bone interface in hips where a metal-on-polyethylene bearing couple has been used. Bone resorption takes place as a result of an inflammatory response to small wear particles. After infection has been excluded the treatment of choice is a single-stage revision♦ Dislocation may be the result of patient factors, implant factors, or poor surgical technique. It is imperative for the clinician to minimize the risk by selecting patients carefully, using the correct combination of implants and performing surgery accurately♦ The management of periprosthetic fractures depends on how well the implants are fixed and quality of bone stock. Treatment ranges from simple fixation of the fracture through to revision augmented with strut allograft.

Efficient fixation procedure of human leukemia cells in sulforhodamine B cytotoxicity assay

1996 ◽  
Vol 36 (3) ◽  
pp. 163-169 ◽  
Author(s):  
Hwan Mook Kim ◽  
Sang Bea Han ◽  
Moon Soon Kim ◽  
Jong Seong Kang ◽  
Goo Taeg Oh ◽  
...  
Keyword(s):  
Cytotoxicity Assay ◽  
Leukemia Cells ◽  
Human Leukemia ◽  
Fixation Procedure ◽  
Sulforhodamine B ◽  
Human Leukemia Cells

The Effects of Foveal Load on Peripheral Visual Sensitivity

1986 ◽  
Vol 30 (6) ◽  
pp. 608-610 ◽  
Author(s):  
Edward J. Rinalducci ◽  
Paul N. Rose
Keyword(s):  
Visual Field ◽  
Visual Sensitivity ◽  
Tracking Task ◽  
Horizontal Meridian ◽  
Experimental Conditions ◽  
First Order ◽  
Order Rate ◽  
Light Flashes ◽  
Simple Fixation

The main objective of the research was to investigate the effects of load on peripheral sensitivity in the visual field. Foveal load was varied by using simple fixation of a vs. a first-order (rate) compensatory tracking task. Peripheral sensitivity was determined simultaneously for light flashes located at different eccentricities along the horizontal meridian. The effects of training on the tracking task were also examined. In general, the results showed no losses in peripheral sensitivity under the experimental conditions employed, contrary to data from previous studies.

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