Histochemical determination of histone and non-histone protein content in rat liver nuclei

1980 ◽  
Vol 68 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Wilma M. Frederiks ◽  
Adri Slob ◽  
Marion Schr�der
Keyword(s):  
Protein Content ◽  
Rat Liver ◽  
Histone Protein ◽  
Rat Liver Nuclei ◽  
Liver Nuclei

scholarly journals CYTOFLUOROMETRIC DETERMINATION OF LYSINE WITH DANSYLCHLORIDE

1968 ◽  
Vol 16 (7) ◽  
pp. 459-466 ◽  
Author(s):  
A. ROSSELET ◽  
F. RUCH
Keyword(s):  
Rat Liver ◽  
Lysine Content ◽  
Amino Groups ◽  
Isolated Rat Liver ◽  
Model Experiments ◽  
Rat Liver Nuclei ◽  
Liver Nuclei ◽  
Isolated Rat

Dansylchloride (l-dimethylamino-naphthalene-5-sulfochloride) may be used for cytofluorometric determination of lysine. By means of model experiments on protein smears it is shown that the reaction must be carried out in ethanol if it is to be specific for amino groups. The fluorescence given by isolated rat liver nuclei treated with dansylchloride corresponds to the three classes of 2n, 4n and 8n nuclei. The dansylfluorescence of several kinds of sperms is proportional to their lysine content. In rat liver nuclei, 95% of the lysine is dansylated and the lysine content may be determined in absolute values by comparison with polylysine. In spermatozoa only 50% of the lysine reacts.

INTERFERENCE OF AUTOLYTIC ACTIVITY WITH THE DETERMINATION OF CELL NUCLEUS COMPOSITION UNDER DIFFERENT CONDITIONS OF PROTEIN DEFICIENCY

1966 ◽  
Vol 44 (2) ◽  
pp. 187-195 ◽  
Author(s):  
Roberto Umaña
Keyword(s):  
Rat Liver ◽  
Cell Nucleus ◽  
Protein Deficiency ◽  
Protein Fractions ◽  
Histone Fraction ◽  
Experimental Conditions ◽  
Rat Liver Nuclei ◽  
Autolytic Activity ◽  
Liver Nuclei

The changes produced by protein-deficient diets and starvation on the composition of rat liver nuclei have been studied. It was found that the histone fraction and the histone to DNA ratio remained remarkably constant during the different experimental conditions studied. The rest of the protein fractions showed variations which can probably be ascribed to an increase in the autolytic activity of the preparations. It appears that the degree of autolysis varies with the type of dietary insult applied to the animal.

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