An X-ray microanalytical azo dye technique for the localization of acid phosphatase activity

1976 ◽  
Vol 49 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Ivor D. Bowen ◽  
Timothy A. Ryder ◽  
Nerys L. Downing
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Azo Dye ◽  
X Ray

scholarly journals HISTOCHEMICAL DEMONSTRATION OF ACID PHOSPHATASE ACTIVITY IN OSTEOCLASTS

1959 ◽  
Vol 7 (1) ◽  
pp. 39-41 ◽  
Author(s):  
M. S. BURSTONE
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Comparative Studies ◽  
Acid Phosphatase Activity ◽  
Azo Dye ◽  
Histochemical Demonstration ◽  
Accurate Localization ◽  
Acid And Alkaline Phosphatase ◽  
Cold Acetone

High acid phosphatase activity was observed in osteoclasts of several species using a reproducible azo-dye technique. High activity of two distinct enzymes, acid and alkaline phosphatase, are associated with osteoclasts and osteoblasts respectivey. Althouth frozen-dried tissues are recommended for definitive studies, the enzyme techniques used give satisfactory results with cold acetone-fixed tissues. The most accurate localization of acid phosphatase in osteoclasts in controlled comparative studies is obtained with double-embedded frozen-dried undecalcified tissues in conjunction with naphthol AS-phosphates.

scholarly journals Aluminum phosphate visualisation of acid phosphatase activity: a biochemical and x-ray microanalysis study.

1982 ◽  
Vol 30 (1) ◽  
pp. 86-90 ◽  
Author(s):  
J P Berry ◽  
J Hourdry ◽  
M Sternberg ◽  
P Galle
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Rat Kidney ◽  
Aluminum Phosphate ◽  
Proximal Tubules ◽  
New Method ◽  
X Ray ◽  
Biochemical Studies

A new method is described that demonstrates acid phosphatase activity in the cells of the proximal tubules of the rat kidney. The method is based on the formation of an insoluble aluminum phosphate precipitate. Microanalysis was used to demonstrate the presence of intracellular aluminum and determine the quantity present under the probe. Parallel biochemical studies showed that the aluminum precipitate was indeed due to acid phosphatase activity.

scholarly journals A NEW HISTOCHEMICAL METHOD FOR ACID PHOSPHATASE BY THE USE OF 5-IODOINDOXYL PHOSPHATE

1966 ◽  
Vol 14 (2) ◽  
pp. 171-176 ◽  
Author(s):  
G. W. EVANS ◽  
CECILIA L. WHINNEY ◽  
K. C. TSOU
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Azo Dye ◽  
Redox System ◽  
Histochemical Demonstration ◽  
Crystal Formation ◽  
Frozen Sections ◽  
Fresh Frozen ◽  
Ph Range

5-Iodoindoxyl phosphate has been found to be a useful indigogenic substrate in the histochemical demonstration of acid phosphatase activity. Its superiority to other indoxyl phosphates is apparently due to a rapid oxidation of 5-iodoindoxyl to 5,5'-diiodoindigo in the acid pH range. A redox system of ferri-ferrocyanide enhances the oxidation and improves the localization. This method can be applied to calcium-formol-fixed tissues or to fresh frozen sections, although fixed tissues yield better results. The method is not recommended for the demonstration of enzyme activity in lipid-rich tissues because of the complexing property of lipids with 5,5'-diiodoindigo that results in crystal formation. The distribution of acid phosphatase activity with this method is generally similar to that obtained using azo dye methods.

DETERMINATION OF ACID PHOSPHATASE ACTIVITY IN TISSUE HOMOGENATES

1966 ◽  
Vol 44 (6) ◽  
pp. 755-761 ◽  
Author(s):  
John P. Manning ◽  
Arthur L. Babson ◽  
Margaret C. Butler ◽  
Sara F. Priester
Keyword(s):  
Small Intestine ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Azo Dye ◽  
Accurate Method ◽  
Acid Phosphate ◽  
Fast Red ◽  
Tissue Homogenates

A simple and accurate method is described for the quantitative determination of acid phosphatase in tissue homogenates, utilizing stable buffered sodium α-naphthyl acid phosphate tablets as substrate, and the monocoupler Fast Red Salt B as the color developer. This technique eliminates deproteinization and ethyl acetate extraction, and produces a highly colored blue azo dye for spectrophotometry. Of the rat organs tested, the spleen had the highest acid phosphatase activity, then kidney, small intestine, liver, lung, and heart, in that order.

An improved procedure for the X-ray microanalysis of acid phosphatase activity in lysosomes

1987 ◽  
Vol 87 (1) ◽  
pp. 71-77 ◽  
Author(s):  
J. B. van Dorf ◽  
J. Ph. Zeelen ◽  
W. C. de Bruijn
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
X Ray
Sign in / Sign up

Export Citation Format

Share Document