A rapid enzymatic micromethod for the determination of intracellular- and extracellular ATP and its clinical-chemical applications

1978 ◽  
Vol 290 (2) ◽  
pp. 180-180 ◽  
Author(s):  
P. Tarkkanen ◽  
R. Driesch ◽  
H. Greiling
Keyword(s):  
Extracellular Atp ◽  
Clinical Chemical ◽  
Enzymatic Micromethod

Automated multiple flow-injection analysis in clinical chemistry: determination of albumin with bromcresol green.

1980 ◽  
Vol 26 (2) ◽  
pp. 331-334 ◽  
Author(s):  
B W Renoe ◽  
K K Stewart ◽  
G R Beecher ◽  
M R Wills ◽  
J Savory
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Clinical Chemistry ◽  
Analytical Results ◽  
Relative Standard ◽  
Clinical Chemical ◽  
Bromcresol Green

Abstract We describe an adaptation of automated multiple flow-injection analysis instrumentation to an analysis for albumin in serum. The bromcresol green reaction was used to test the utility of the system. The approach yielded albumin results with excellent sensitivity, no measurable carryover, a relative standard deviation of less than 1%, good correlations with published procedures, and no measurable interferences. The simplicity and flexibility of the instrumentation and its performance integrity, as indicated by the analytical results, make this a viable clinical chemical tool.

Multicomponent analysis of hemoglobin derivatives with reversed-optics spectrophotometer.

1984 ◽  
Vol 30 (3) ◽  
pp. 373-379 ◽  
Author(s):  
A Zwart ◽  
A Buursma ◽  
E J van Kampen ◽  
W G Zijlstra
Keyword(s):  
Simultaneous Determination ◽  
Separate Determination ◽  
Overdetermined System ◽  
Chemical Research ◽  
Chemical Laboratory ◽  
Multicomponent Analysis ◽  
Hemoglobin Derivatives ◽  
Clinical Chemical ◽  
Human Blood Samples

Abstract We describe a method for the simultaneous determination of the five clinically relevant hemoglobin derivatives (Hb, HbO2, HbCO, Hi, SHb) in a blood sample by means of a reversed-optics spectrophotometer (Hewlett-Packard HP8450 A UV/Vis). A built-in computer program is used for multicomponent analysis in an overdetermined system, i.e., a system in which the number of independent equations used exceeds the number of unknowns to be determined. First, the spectra of the five hemoglobin derivatives are measured in a series of different human blood samples. Thereafter, the multicomponent method for the simultaneous determination of the five hemoglobin derivatives is tested by comparison with conventional methods for the separate determination of oxygen saturation, HbCO, Hi, and SHb fractions. The multicomponent (multiwavelength) method is sufficiently reliable, accurate, and easy to justify its use in physiological chemical research as well as its routine application in the clinical chemical laboratory.

Direct enzymatic determination of urea in plasma and urine with a centrifugal analyzer.

1976 ◽  
Vol 22 (10) ◽  
pp. 1614-1617 ◽  
Author(s):  
J P Bretaudiere ◽  
H T Phung ◽  
M Bailly
Keyword(s):  
Glutamate Dehydrogenase ◽  
Continuous Flow ◽  
Kinetic Scheme ◽  
Fixed Time ◽  
Sample Volume ◽  
Enzymatic Determination ◽  
Coupled Reactions ◽  
Continuous Flow Method ◽  
Enzymatic Micromethod

Abstract A direct enzymatic micromethod (sample volume, 3mul) has been adapted to the centrifugal analyzer (ENI-GEMSAEC) for measurement of urea in plasma and urine. The method is based on urease (urea amidohydrolase, EC3.5.1.5)/glutamate dehydrogenase [l-glutamate:NAD(P)+oxidoreductase (deaminating), EC1.41.3] coupled reactions, and uses a two-point fixed-time (t(1)=20s,t(2)=50s)kinetic scheme for monitoring the rate of comsumption of NADH at 340 nm. Sensitivity and precision of the method are excellent,and results compare well with those from a commonly used continuous-flow method.

A System of Clinical Chemical Analysis

1959 ◽  
Vol 5 (1) ◽  
pp. 62-70 ◽  
Author(s):  
Bernard Klein
Keyword(s):  
Chemical Analysis ◽  
Zinc Sulfate ◽  
Large Scale ◽  
Barium Hydroxide ◽  
Clinical Chemical ◽  
Sodium And Potassium

Abstract A system of analysis has been presented for the determination of glucose, urea N, chloride, sodium, and potassium, using 1.0 ml. of serum. The centrifugate of the barium hydroxide-zinc sulfate deproteinization is utilized for all analyses. The system is readily adaptable to large-scale operations.

scholarly journals Lead - a preanalytical/analytical variable in clinical chemistry

2014 ◽  
Vol 12 (1) ◽  
pp. 65-76
Author(s):  
Ivana Rasic-Misic ◽  
Emilija Pecev-Marinkovic
Keyword(s):  
Lead Poisoning ◽  
Laboratory Tests ◽  
Review Paper ◽  
Clinical Laboratory ◽  
Clinical Chemistry ◽  
Clinical Chemical ◽  
Analytical Variable ◽  
Lead Intake ◽  
Proper Interpretation

Lead is one of the most studied clinically important metals due its high toxicity and a high number of workers exposed to it. The interest toward Pb is elevated by the fact that children are especially susceptible to lead poisoning. Research regarding lead poisoning requires a complex, multi-disciplinary (clinical medical and clinical chemical) approach. Monitoring human exposure to lead (intake, i.e. poisoning) may be achieved by quantification of Pb in tissues and body fluids. For that reason, a number of accurate and reliable analytical methods for the determination of Pb (analytical/preanalytical variable) were developed. An objective of this review paper is to provide key information necessary for proper interpretation of results of lead related clinical/laboratory tests.

Automated multiple flow-injection analysis in clinical chemistry: determination of albumin with bromcresol green.

1980 ◽  
Vol 26 (2) ◽  
pp. 331-334
Author(s):  
B W Renoe ◽  
K K Stewart ◽  
G R Beecher ◽  
M R Wills ◽  
J Savory
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Clinical Chemistry ◽  
Analytical Results ◽  
Relative Standard ◽  
Clinical Chemical ◽  
Bromcresol Green

Abstract We describe an adaptation of automated multiple flow-injection analysis instrumentation to an analysis for albumin in serum. The bromcresol green reaction was used to test the utility of the system. The approach yielded albumin results with excellent sensitivity, no measurable carryover, a relative standard deviation of less than 1%, good correlations with published procedures, and no measurable interferences. The simplicity and flexibility of the instrumentation and its performance integrity, as indicated by the analytical results, make this a viable clinical chemical tool.

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