The clinical-chemical application of the NADH test for the determination of diamine oxidase activity in human pregnancy

1974 ◽  
Vol 4 (3) ◽  
pp. 188-189 ◽  
Author(s):  
J. Kusche ◽  
U. Trotha ◽  
G. Mühlberger ◽  
W. Lorenz
Keyword(s):  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Chemical Application ◽  
Human Pregnancy ◽  
Clinical Chemical ◽  
Diamine Oxidase Activity

Pitfalls in the determination of diamine oxidase activity

1991 ◽  
Vol 201 (1-2) ◽  
pp. 89-94
Author(s):  
Catherine H. Smith ◽  
Mehran Maghsoudloo ◽  
Kazuo Himuna ◽  
Gerard M. Murphy
Keyword(s):  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Diamine Oxidase Activity

The influence of some drugs on the determination of diamine oxidase activity

1979 ◽  
Vol 9 (1) ◽  
pp. 44-45 ◽  
Author(s):  
A. C. Andersson ◽  
S. Henningsson ◽  
E. Rosengren
Keyword(s):  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Diamine Oxidase Activity

scholarly journals In vitro determination of diamine oxidase activity in food matrices by an enzymatic assay coupled to UHPLC-FL

2019 ◽  
Vol 411 (28) ◽  
pp. 7595-7602 ◽  
Author(s):  
Oriol Comas-Basté ◽  
M. Luz Latorre-Moratalla ◽  
Sònia Sánchez-Pérez ◽  
M. Teresa Veciana-Nogués ◽  
M. Carmen Vidal-Carou
Keyword(s):  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Enzymatic Assay ◽  
Diamine Oxidase Activity ◽  
Food Matrices

scholarly journals Determination of plasma diamine oxidase activity by HPLC method and effect of food intake on it.

1993 ◽  
Vol 61 ◽  
pp. 197
Author(s):  
Ikuo Imamura ◽  
Shinya Kondo ◽  
Yoshiji Miyazaki ◽  
Atsushi Yamatodani ◽  
Hiroyuki Fukui
Keyword(s):  
Food Intake ◽  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Hplc Method ◽  
Diamine Oxidase Activity ◽  
Effect Of Food

scholarly journals Spermidine oxidase in human pregnancy serum. Probable identity with diamine oxidase

1982 ◽  
Vol 201 (1) ◽  
pp. 161-166 ◽  
Author(s):  
W A Gahl ◽  
A M Vale ◽  
H C Pitot
Keyword(s):  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Gel Filtration ◽  
Exchange Chromatography ◽  
Ph Optimum ◽  
Human Pregnancy ◽  
Diamine Oxidase Activity ◽  
Radioactive Assay ◽  
Spermidine Oxidase ◽  
In Pregnancy

Diamine oxidase was previously measured in human pregnancy serum with putrescine or histamine as substrate. We have now documented the presence of spermidine oxidase activity in pregnancy serum by means of a specific radioactive assay with [14C]spermidine as substrate and Dowex 50 cation-exchange chromatography to separate products from substrate. The apparent Km of a partially purified preparation of this enzyme for spermidine was 10.9 microM and the Ki for aminoguanidine was 0.8 microM. The pH optimum (pH 9.0) and temperature optimum (55 degrees C) were identical with those for diamine oxidase. Spermidine oxidase activity and diamine oxidase activity eluted in a concerted fashion when pregnancy serum was subjected to cadaverine-Sepharose chromatography, gel filtration and ion-exchange chromatography. Spermidine oxidase became detectable in serum during pregnancy in the human approx. 8 weeks after the last menstrual period and increased with gestational age in concert with the increase in diamine oxidase activity, reaching a plateau at 20 weeks of gestation. Foetal-cord serum displayed virtually no activity of either enzyme. A 400-fold-purified preparation of diamine oxidase retained the same diamine oxidase/spermidine oxidase ratio as exhibited by crude pregnancy serum. These data suggest that in pregnancy serum, unlike foetal bovine serum, spermidine oxidase and diamine oxidase activity may be a single enzyme protein.

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