Evidence for the involvement and role of a corrinoid enzyme in methane formation from acetate in Methanosarcina barkeri

1985 ◽  
Vol 142 (2) ◽  
pp. 175-179 ◽  
Author(s):  
Bernhard Eikmanns ◽  
Rudolf K. Thauer
Keyword(s):  
Methanosarcina Barkeri ◽  
Methane Formation ◽  
Corrinoid Enzyme

Pyruvate ? a novel substrate for growth and methane formation in Methanosarcina barkeri

1994 ◽  
Vol 161 (1) ◽  
pp. 33-46 ◽  
Author(s):  
Anne-Katrin Bock ◽  
Angelika Prieger-Kraft ◽  
Peter Sch�nheit
Keyword(s):  
Methanosarcina Barkeri ◽  
Methane Formation

The role of zinc in the methylation of the coenzyme M thiol group in methanol:coenzyme M methyltransferase from Methanosarcina barkeri

2002 ◽  
Vol 269 (8) ◽  
pp. 2117-2123 ◽  
Author(s):  
Markus Krüer ◽  
Michael Haumann ◽  
Wolfram Meyer-Klaucke ◽  
Rudolf K. Thauer ◽  
Holger Dau
Keyword(s):  
Thiol Group ◽  
Methanosarcina Barkeri ◽  
Coenzyme M

scholarly journals In vivo 31P-and 13C-NMR studies of ATP synthesis and methane formation by Methanosarcina barkeri

1989 ◽  
Vol 180 (2) ◽  
pp. 421-427 ◽  
Author(s):  
Helena SANTOS ◽  
Paula FARELEIRA ◽  
Rene TOCI ◽  
Jean LeGALL ◽  
Harry D. PECK ◽  
...  
Keyword(s):  
13C Nmr ◽  
Atp Synthesis ◽  
Methanosarcina Barkeri ◽  
Methane Formation ◽  
Nmr Studies

Mechanism of ATP sythesis and role of sodium ions in Methanosarcina barkeri growing on methanol

1986 ◽  
Vol 7 (2-3) ◽  
pp. 354-357 ◽  
Author(s):  
Michael Blaut ◽  
Volker Müller ◽  
Gerhard Gottschalk
Keyword(s):  
Methanosarcina Barkeri ◽  
Sodium Ions

scholarly journals Secondary Mineralization of Ferrihydrite Affects Microbial Methanogenesis in Geobacter-Methanosarcina Cocultures

2016 ◽  
Vol 82 (19) ◽  
pp. 5869-5877 ◽  
Author(s):  
Jia Tang ◽  
Li Zhuang ◽  
Jinlian Ma ◽  
Ziyang Tang ◽  
Zhen Yu ◽  
...  
Keyword(s):  
Iron Oxides ◽  
Biogeochemical Cycling ◽  
Methanosarcina Barkeri ◽  
Geobacter Metallireducens ◽  
Methanogenic Activity ◽  
Content Type ◽  
Iron Minerals ◽  
Iron Mineral ◽  
Iron Mineralogy

ABSTRACTThe transformation of ferrihydrite to stable iron oxides over time has important consequences for biogeochemical cycling of many metals and nutrients. The response of methanogenic activity to the presence of iron oxides depends on the type of iron mineral, but the effects of changes in iron mineralogy on methanogenesis have not been characterized. To address these issues, we constructed methanogenic cocultures ofGeobacterandMethanosarcinastrains with different ferrihydrite mineralization pathways. In this system, secondary mineralization products from ferrihydrite are regulated by the presence or absence of phosphate. In cultures producing magnetite as the secondary mineralization product, the rates of methanogenesis from acetate and ethanol increased by 30.2% and 135.3%, respectively, compared with a control lacking ferrihydrite. Biogenic magnetite was proposed to promote direct interspecies electron transfer betweenGeobacterandMethanosarcinain a manner similar to that ofc-type cytochrome and thus facilitate methanogenesis. Vivianite biomineralization from ferrihydrite in the presence of phosphate did not significantly influence the methanogenesis processes. The correlation between magnetite occurrence and facilitated methanogenesis was supported by increased rates of methane production from acetate and ethanol with magnetite supplementation in the defined cocultures. Our data provide a new perspective on the important role of iron biomineralization in biogeochemical cycling of carbon in diverse anaerobic environments.IMPORTANCEIt has been found that microbial methanogenesis is affected by the presence of iron minerals, and their influences on methanogenesis are associated with the mineralogical properties of the iron minerals. However, how changes in iron mineralogy affect microbial methanogenesis has not been characterized. To address this issue, we constructed methanogenic cocultures ofGeobacterandMethanosarcinastrains with different ferrihydrite mineralization pathways. The experimental results led to two contributions, i.e., (i) the transformation of iron minerals might exert an important influence on methanogenesis under anaerobic conditions and (ii) both biogenic and chemical magnetite can accelerate syntrophic ethanol oxidization betweenGeobacter metallireducensandMethanosarcina barkeri. This study sheds new light on the important role of iron biomineralization in the biogeochemical cycling of carbon in diverse anaerobic environments, particularly in iron-rich natural and agricultural wetland soils.

Flocculation in methanogens, a comparative study of Methanosarcina barken strains Jülich and Fusaro

1986 ◽  
Vol 32 (2) ◽  
pp. 137-144 ◽  
Author(s):  
P. A. Scherer ◽  
H. -P. Bochem ◽  
J. D. Davis ◽  
D. C. White
Keyword(s):  
Glucuronic Acid ◽  
Large Cell ◽  
Growth Conditions ◽  
Calcium Deficiency ◽  
Methanosarcina Barkeri ◽  
Uronic Acids ◽  
Poor Growth ◽  
Bread Crumb ◽  
Cell Aggregations

Two strains of Methanosarcina barkeri grown on methanol as substrate were investigated for their ability to aggregate: the new, flocculent strain Jülich formed stable flocs of several millimetres in diameter during rapid growth on a methanol-containing medium ("bread-crumb growth"). When observed with an electron microscope, the Jülich strain showed a unique parenchymatic texture with thick-walled cells inside a floc, and coccoidlike cells on the periphery. In contrast to the Jülich strain, the strain Fusaro grew on methanol in dispersed form, and generated macroscopically visible clumps only under poor growth conditions, induced, for example, by calcium deficiency. The formation of large cell aggregations of the Fusaro strain could also be induced during growth in the presence of 0.01% of the stain Calcofluor which is known to interact specifically with β-1,4 and β-1,3 glucan moieties. Sugar analyses revealed a different pattern for both strains: the exopolymer of the flocculent strain Jülich contained half the rhamnose, a third the fucose, equal amounts of mannose and glucose, but four times more glucuronic acid and arabinose than the strain Fusaro grown in the dispersed form. The high proportion of glucuronic acid in the exopolymer of the flocculating form is of particular interest because of the role of uronic acids in adhesion.

Sign in / Sign up

Export Citation Format

Share Document