Pyruvate ? a novel substrate for growth and methane formation in Methanosarcina barkeri

1994 ◽  
Vol 161 (1) ◽  
pp. 33-46 ◽  
Author(s):  
Anne-Katrin Bock ◽  
Angelika Prieger-Kraft ◽  
Peter Sch�nheit
Keyword(s):  
Methanosarcina Barkeri ◽  
Methane Formation

scholarly journals In vivo 31P-and 13C-NMR studies of ATP synthesis and methane formation by Methanosarcina barkeri

1989 ◽  
Vol 180 (2) ◽  
pp. 421-427 ◽  
Author(s):  
Helena SANTOS ◽  
Paula FARELEIRA ◽  
Rene TOCI ◽  
Jean LeGALL ◽  
Harry D. PECK ◽  
...  
Keyword(s):  
13C Nmr ◽  
Atp Synthesis ◽  
Methanosarcina Barkeri ◽  
Methane Formation ◽  
Nmr Studies

Dissimilatory sulphate reduction with acetate as electron donor

1982 ◽  
Vol 298 (1093) ◽  
pp. 467-471 ◽  
Keyword(s):  
Citric Acid Cycle ◽  
Citrate Synthase ◽  
Methanosarcina Barkeri ◽  
Methane Formation ◽  
Acetate Oxidation ◽  
Natural Habitats ◽  
Cell Extracts ◽  
Pyruvate Synthase ◽  
Desulfobacter Postgatei

Acetate oxidation by sulphate was studied with Desulfobacter postgatei . Cell extracts of the organism were found to contain high activities of the following enzymes: citrate synthase, aconitase, isocitrate dehydrogenase, a-ketoglutarate dehydrogenase, succinate dehydrogenase, fumarase, malate dehydrogenase and pyruvate synthase. It is concluded that acetate oxidation with sulphate in D. postgatei proceeds via the citric acid cycle with the synthesis of pyruvate from acetyl CoA and CO 2 as an anaplerotic reaction. The apparent K s for acetate oxidation by D. postgatei as determined in vivo was near 0.2 mM. The apparent Ks for acetate fermentation to methane and CO 2 by Methanosarcina barkeri was 3 mM. The significantly lower K s for acetate of the sulphate reducer explains why methane formation from acetate in natural habitats is apparently inhibited by sulphate.

Pyruvate ? a novel substrate for growth and methane formation in Methanosarcina barkeri

1994 ◽  
Vol 161 (1) ◽  
pp. 33-46 ◽  
Author(s):  
A.-K. Bock ◽  
A. Prieger-Kraft ◽  
P. Sch�nheit
Keyword(s):  
Methanosarcina Barkeri ◽  
Methane Formation

scholarly journals Acetogens and Acetoclastic Methanosarcinales Govern Methane Formation in Abandoned Coal Mines

2011 ◽  
Vol 77 (11) ◽  
pp. 3749-3756 ◽  
Author(s):  
Sabrina Beckmann ◽  
Tillmann Lueders ◽  
Martin Krüger ◽  
Frederick von Netzer ◽  
Bert Engelen ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Coal Mines ◽  
Methanogenic Archaea ◽  
Stable Isotope Probing ◽  
Methanosarcina Barkeri ◽  
Hard Coal ◽  
Methane Formation ◽  
Content Type ◽  
Abandoned Coal Mines ◽  
Trophic Network

ABSTRACTIn abandoned coal mines, methanogenic archaea are responsible for the production of substantial amounts of methane. The present study aimed to directly unravel the active methanogens mediating methane release as well as active bacteria potentially involved in the trophic network. Therefore, the stable-isotope-labeled precursors of methane, [13C]acetate and H2-13CO2, were fed to liquid cultures from hard coal and mine timber from a coal mine in Germany. Guided by methane production rates, samples for DNA stable-isotope probing (SIP) with subsequent quantitative PCR and denaturing gradient gel electrophoretic (DGGE) analyses were taken over 6 months. Surprisingly, the formation of [13C]methane was linked to acetoclastic methanogenesis in both the [13C]acetate- and the H2-13CO2-amended cultures of coal and timber. H2-13CO2was used mainly by acetogens related toPelobacter acetylenicusandClostridiumspecies. Active methanogens, closely affiliated withMethanosarcina barkeri, utilized the readily available acetate rather than the thermodynamically more favorable hydrogen. Thus, the methanogenic microbial community appears to be highly adapted to the low-H2conditions found in coal mines.

BIOGAS PRODUCTION IN THE CONCENTRATED DISTILLERY WASTEWATER TREATMENT

2018 ◽  
pp. 51-59 ◽  
Author(s):  
N. Golub ◽  
M. Potapova ◽  
M. Shinkarchuk ◽  
O. Kozlovets
Keyword(s):  
Wastewater Treatment ◽  
Dry Matter ◽  
Ph Value ◽  
Biogas Production ◽  
Anaerobic Sludge ◽  
Methane Formation ◽  
Maximum Output ◽  
Processing Technologies ◽  
Spent Wash ◽  
Distillery Spent Wash

The paper deals with the waste disposal problem of the alcohol industry caused by the widespread use of alcohol as biofuels. In the technology for the production of alcohol from cereal crops, a distillery spent wash (DSW) is formed (per 1 dm3 of alcohol – 10–20 dm3 DSW), which refers to highly concentrated wastewater, the COD value reaches 40 g O2/dm3. Since the existing physical and chemical methods of its processing are not cost-effective, the researchers develop the processing technologies for its utilization, for example, an anaerobic digestion. Apart from the purification of highly concentrated wastewater, the advantage of this method is the production of biogas and highquality fertilizer. The problems of biotechnology for biogas production from the distillery spent wash are its high acidity–pH 3.7–5.0 (the optimum pH value for the methanogenesis process is 6.8–7.4) and low nitrogen content, the lack of which inhibits the development of the association of microorganisms. In order to solve these problems, additional raw materials of various origins (chemical compounds, spent anaerobic sludge, waste from livestock farms, etc.) are used. The purpose of this work is to determine the appropriate ratio of the fermentable mixture components: cosubstrate, distillery spent wash and wastewater of the plant for co-fermentation to produce an energy carrier (biogas) and effective wastewater treatment of the distillery. In order to ensure the optimal pH for methanogenesis, poultry manure has been used as a co-substrate. The co-fermentation process of DSW with manure has been carried out at dry matter ratios of 1:1, 1:3, 1:5, 1:7 respectively. It is found that when the concentration of manure in the mixture is insufficient (DSW/manure – 1:1, 1:3), the pH value decreases during fermentation which negatively affects methane formation; when the concentration of manure in the mixture is increased (DSW/manure – 1:5, 1:7), the process is characterized by a high yield of biogas and methane content. The maximum output of biogas with a methane concentration of 70 ± 2% is observed at the ratio of components on a dry matter “wastewater: DSW: manure” – 0,2:1:7 respectively. The COD reduction reaches a 70% when using co-fermentation with the combination of components “wastewater: DSW: manure” (0,3:1:5) respectively.

DISTRIBUTION OF DISSOLVED METHANE IN SURFACE COASTAL WATERS OF THE NORTHEASTERN PART OF THE BLACK SEA

2017 ◽  
Author(s):  
Elena Kovaleva ◽  
Elena Kovaleva ◽  
Alexander Izhitskiy ◽  
Alexander Izhitskiy ◽  
Alexander Egorov ◽  
...  
Keyword(s):  
Black Sea ◽  
Natural Waters ◽  
Sea Water ◽  
The Black Sea ◽  
Methane Formation ◽  
Anthropogenic Pressure ◽  
Russian Science ◽  
Southeastern Part ◽  
Dissolved Methane ◽  
Continental Runoff

Studying of methane formation and distribution in natural waters is important for understanding of biogeochemical processes of carbon cycle, searching for oil and gas sections and evaluation of CH4 emissions for investigations of greenhouse effect. The Black Sea is the largest methane water body on our planet. However, relatively low values of methane concentration (closed to equilibrium with the atmospheric air) are typical of the upper aerobic layer. At the same time, the distribution pattern of CH4 in surface waters of coastal areas is complicated by the influence of coastal biological productivity, continental runoff, bottom sources, hydrodynamic processes and anthropogenic effect. The investigation is focused on the spatial variability of dissolved methane in the surface layer of the sea in coastal regions affected by the continental runoff and anthropogenic pressure. Unique in situ data on methane concentrations were collected along the ship track on 2 sections between Sochi and Gelendzhik (2013, 2014) and 2 sections between Gelendzhik and Feodosia (2015). Overall 170 samples were obtained. Gas-chromatographic analysis of the samples revealed increase of CH4 saturation in the southeastern part of the Crimean shelf and the Kerch Strait area. Such a pattern was apparently caused by the influence of the Azov Sea water spread westward along the Crimean shore from the strait. This work was supported by the Russian Science Foundation, Project 14-50-00095 and the Russian Foundation for Basic Research, Project 16-35-00156 mol_a.

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