Feinstrukturanalyse der komplexen Gei�eln von Rhizobium lupini H 13-3

1974 ◽  
Vol 100 (1) ◽  
pp. 145-162 ◽  
Author(s):  
R�diger Schmitt ◽  
Ingeborg Bamberger ◽  
Georg Acker ◽  
Frank Mayer
Keyword(s):  
Rhizobium Lupini

SENSITIVITY AND ADAPTATION OF SELECTED RHIZOBIA AND AGROBACTERIA TO PARAQUAT

1985 ◽  
Vol 65 (3) ◽  
pp. 555-562 ◽  
Author(s):  
E. B. ROSLYCKY
Keyword(s):  
Key Words ◽  
Genetic Effect ◽  
Agrobacterium Radiobacter ◽  
Plant Weight ◽  
Fast Growing ◽  
Plant Symbiont ◽  
Paraquat Resistance ◽  
Rhizobium Lupini ◽  
The Relationship ◽  
Slow Growing

Sensitivity to paraquat as function of growth varied considerably among 15 rhizobia from seven cross-inoculation groups and 13 agrobacteria from five different species. No relationship was evident between the sensitivity and the "fast-growing" or the "slow-growing" characteristics among the rhizobia. The agrobacteria were uniformly less sensitive. Maximum stabilized paraquat resistance induced by the adptations ranged from 200 μg paraquat∙mL−1 in Rhizobium lupini 112, to 20 000 μg paraquat∙mL−1 in Agrobacterium radiobacter R-590 and A. tumefaciens A6-K1. No relationship was found between the sensitivity and the adaptability. Identical lytic patterns with six bacteriophages confirmed the relationship between the adapted and parent cultures suggesting no genetic effect of paraquat on susceptibility to lysis. The bacteria-plant symbiont interaction, including nodulation, plant weight, vigor and color, was unaffected by the adaptations to paraquat resistance. Similarly, the adaptations exerted no effect on tumorigenicity of A. tumefaciens A6-K1. Key words: Rhizobia, agrobacteria, adaptation, herbicides, paraquat, N2-fixation

Nodulation studies on legumes exotic to Australia: Lupinus and Ornithopus spp

1986 ◽  
Vol 26 (1) ◽  
pp. 37 ◽  
Author(s):  
RR Gault ◽  
EJ Corbin ◽  
KA Boundy ◽  
J Brockwell
Keyword(s):  
Nitrogen Fixation ◽  
Field Experiments ◽  
Gum Arabic ◽  
Significant Loss ◽  
Growth And Yield ◽  
Seed Coating ◽  
Single Strain ◽  
Taxonomic Relationship ◽  
The Third ◽  
Rhizobium Lupini

In a series of glasshouse and field experiments, the symbiotic characteristics of 24 lines of Lupinus and Ornithopus species and 20 strains of Rhizobium lupini were defined. Rhizobium lupini inoculant established readily in several soils and lupins grown in the field responded to inoculation by improved nodulation, growth and yield. It is concluded that lupin crops sown on new land need to be inoculated to achieve optimum yield. At three sites, field-grown lupins responded to increasing rates of inoculation up to the rate recommended by the inoculant manufacturer. At two of the sites there was no further response to higher rates, but at the third there was a continuing response up to 125x (inoculation rate). Lupin seed was preinoculated, using gum arabic adhesive, up to 33 days before sowing without significant loss of viability or nodulating capacity of the inoculant. Seed coating with several materials did not improve inoculant viability on preinoculated seed. In glasshouse experiments, hostxstrain interactions in nitrogen fixation were frequent and substantial. They occurred at three levels of taxonomic relationship, viz, between the genera Lupinus and Ornithopus, between different species within the same genus, and between different lines of the same lupin species. Hostx strain interactions were also observed in field experiments but were less frequent and smaller than in the glasshouse. These observations have implications for the 'single-strain inoculant policy that applies to the manufacture of commercial lupin and serradella inoculant in Australia.

The narrow-leafed lupin (Lupinus angustifolius L.) as a nitrogen-fixing rotation crop for cereal production. I. Indices of nitrogen fixation

1988 ◽  
Vol 39 (6) ◽  
pp. 1003 ◽  
Author(s):  
DF Herridge
Keyword(s):  
N2 Fixation ◽  
Plant Size ◽  
Lupinus Angustifolius ◽  
Xylem Exudate ◽  
Plant Parts ◽  
Rooting Medium ◽  
Rhizobium Lupini ◽  
Nitrate Supply ◽  
Root Tissues ◽  
Rotation Crop

Experiments to develop indices of N2 fixation activity for the narrow-leafed lupin (Lupinus angustifolius L.) are reported. In Experiment 1 Unicrop narrow-leafed lupins were inoculated at sowing with effective Rhizobium lupini WU425. The conversion factor relating C2H2 reduction to N2 fixation was not constant throughout growth but increased from 0.9 (50-60 day period) to 6.6 (110-120 days). A nodulation index [(nodule wt/shoot wt) x 100], developed to account for plant size, declined with increasing nitrate supply and with increasing plant age. However, minor shifts in the nodulation index represented large shifts in plant dependence on N2 fixation (p), when plants were 60-100% dependent on N2 fixation. Concentrations of nitrate in extracts of the shoot axes and nodulated roots increased with increasing nitrate supply. Although the presence of nitrate in the rooting medium was evidenced by its presence in xylem exudate, the relationship between nitrate supply and the nitrate contents of xylem exudate was generally poor. Functions were developed to describe the relationships between the nodulation index and p, and between both shoot and root nitrate and p. Other experiments highlighted the synchrony of nitrate supply and its appearance in root tissues and the lack of diurnal fluctuations in nitrate concentrations of plant parts.

Rhizobial inoculant for iprodione-treated lupin seed: evaluation of an iprodine-resistant Rhizobium lupini

1989 ◽  
Vol 29 (5) ◽  
pp. 641 ◽  
Author(s):  
J Evans ◽  
GE O'Connor ◽  
G Griffith ◽  
J Howieson
Keyword(s):  
Seed Treatment ◽  
Dry Matter ◽  
Field Trials ◽  
Sand Culture ◽  
Late Winter ◽  
Soil N ◽  
Early Winter ◽  
Tolerance Level ◽  
Rhizobium Lupini ◽  
Commercial Inoculant

Lupin nodulation is reduced when seed of this legume is treated with commercial inoculant (Rhizobium lupini WU425) and the fungicide Rovral (a.i. iprodione). The number of WU425 colonising soil beneath lupin seedlings established without iprodione was greater than where iprodione was used. R. lupini CC606B has greater tolerance of iprodione than WU425; 85% of CC606B survived 15 min in iprodione (Rovral 16 g/L), compared with <1% survival of WU425. CC606B fixed as much nitrogen as WU425 on serradella grown in sand culture, but it nodulated lupin poorly in the field. It was shown that CC606B comprised bacteria that were either positive or negative for nodulation of serradella and lupin. Thus, an isolate of CC606B (CC606B/1), able to form nodules on lupin and serradella and also tolerant of iprodione (Rovral 16 g/L), was selected and compared with WU425 as inoculants of lupin, in field trials with and without iprodione seed treatment. In the treatments without the fungicide, CC606B/1, like its parent, failed to nodulate lupin as extensively as WU425. CC606B/1 was unable to colonise soil beneath lupin crops as well as WU425. With iprodione, nodulation by CC606B/1 was significantly worse than without the fungicide; so the tolerance level of CC606B/1 to iprodione was insufficient. With these treatments it was shown that nodule abundance in late winter was related to the abundance of rhizobia in the soil beneath lupin seedlings in early winter. When there were fewer nodules, less dry matter was produced; but grain yield was not affected by the reductions in nodulation and dry matter. The implications of these changes on N2 fixation and lupin effects on soil N are discussed. Other species and strains of Rhizobium varied in their ability to survive iprodione.

Strain of Rhizobium lupini determines natural abundance of 15N in root nodules of Lupinus spp

1986 ◽  
Vol 18 (1) ◽  
pp. 97-101 ◽  
Author(s):  
F.J. Bergersen ◽  
G.L. Turner ◽  
Noelle Amarger ◽  
Francoise Mariotti ◽  
A. Mariotti
Keyword(s):  
Root Nodules ◽  
Natural Abundance ◽  
Rhizobium Lupini

scholarly journals Properties of Antisera to Glutamate Dehydrogenase from Nitrogen-fixing Lupin Nodules

1985 ◽  
Vol 38 (1) ◽  
pp. 51 ◽  
Author(s):  
JR Kennedy ◽  
Z Wyszomirska-Dreher ◽  
Y T T chan ◽  
JW Chen
Keyword(s):  
Enzyme Activity ◽  
Glutamate Dehydrogenase ◽  
Bovine Liver ◽  
Lupinus Luteus ◽  
Plant Origin ◽  
Nitrogen Fixing ◽  
Leguminous Plants ◽  
Rhizobium Lupini

Antibody prepared in rabbit to lupin (Lupinus luteus) nodule glutamate dehydrogenase (GDH) crossreacted with all six isozymes of GDH isolated from lupin nodules. Rocket immunoelectrophoresis showed that the antisera were also strongly cross-reactive with GDH from other parts of the lupin plant and from the roots and stems of other leguminous plants and wheat, but not with GDH of Rhizobium lupini, lupin bacteroids or bovine liver. This confirms the exclusively plant origin of lupin nodule cytosolic GDH. Enzyme activity, determined spectrophotometrically, was strongly inhibited by the antibody. Substrates and modifiers of GDH did not influence the degree of this inhibition, indicating that the antiserum should be an effective reagent for study of the localization of GDH in plants.

Sign in / Sign up

Export Citation Format

Share Document