Rhizobial inoculant for iprodione-treated lupin seed: evaluation of an iprodine-resistant Rhizobium lupini

1989 ◽  
Vol 29 (5) ◽  
pp. 641 ◽  
Author(s):  
J Evans ◽  
GE O'Connor ◽  
G Griffith ◽  
J Howieson

Lupin nodulation is reduced when seed of this legume is treated with commercial inoculant (Rhizobium lupini WU425) and the fungicide Rovral (a.i. iprodione). The number of WU425 colonising soil beneath lupin seedlings established without iprodione was greater than where iprodione was used. R. lupini CC606B has greater tolerance of iprodione than WU425; 85% of CC606B survived 15 min in iprodione (Rovral 16 g/L), compared with <1% survival of WU425. CC606B fixed as much nitrogen as WU425 on serradella grown in sand culture, but it nodulated lupin poorly in the field. It was shown that CC606B comprised bacteria that were either positive or negative for nodulation of serradella and lupin. Thus, an isolate of CC606B (CC606B/1), able to form nodules on lupin and serradella and also tolerant of iprodione (Rovral 16 g/L), was selected and compared with WU425 as inoculants of lupin, in field trials with and without iprodione seed treatment. In the treatments without the fungicide, CC606B/1, like its parent, failed to nodulate lupin as extensively as WU425. CC606B/1 was unable to colonise soil beneath lupin crops as well as WU425. With iprodione, nodulation by CC606B/1 was significantly worse than without the fungicide; so the tolerance level of CC606B/1 to iprodione was insufficient. With these treatments it was shown that nodule abundance in late winter was related to the abundance of rhizobia in the soil beneath lupin seedlings in early winter. When there were fewer nodules, less dry matter was produced; but grain yield was not affected by the reductions in nodulation and dry matter. The implications of these changes on N2 fixation and lupin effects on soil N are discussed. Other species and strains of Rhizobium varied in their ability to survive iprodione.

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 879
Author(s):  
Seong-Shin Lee ◽  
Jeong-Seok Choi ◽  
Dimas Hand Vidya Paradhipta ◽  
Young-Ho Joo ◽  
Hyuk-Jun Lee ◽  
...  

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.


Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1079
Author(s):  
Martina Cardoni ◽  
Jesús Mercado-Blanco ◽  
Rafael Villar

Verticillium wilt of olive (VWO), caused by the soil-borne pathogen Verticillium dahliae, is considered one of the most important diseases affecting this tree crop. One of the best VWO management measures is the use of tolerant cultivars. Remarkably, no information is available about olive functional traits and their potential relationship with tolerance to V. dahliae. Twenty-five selected functional traits (for leaf, stem, root and whole plant) were evaluated in six olive varieties differing in their VWO tolerance level to identify possible links between this phenotype and functional traits’ variation. High intervarietal diversity was found among cultivars and several functional traits were related with VWO tolerance. Tolerant varieties showed higher leaf area, dry matter content (leaf, stem and plant) and mass fraction for stems, but lower for leaves. Significant differences were also detected for root functional traits, tolerant cultivars displaying larger fine root diameter and lignin content but smaller specific length and area of thick and fine roots. Correlations were found among functional traits both within varieties and between levels of tolerance/susceptibility to VWO. Associations were observed between biomass allocation, dry matter content and VWO tolerance. The most relevant difference between tolerant and susceptible cultivars was related to root system architecture.


2021 ◽  
Author(s):  
Hyung-Ju Park ◽  
Kwang-Yul Kim

AbstractEffect of global warming on the sub-seasonal variability of the Northern Hemispheric winter (NDJFM) Pacific storm-track (PST) activity has been investigated. Previous studies showed that the winter-averaged PST has shifted northward and intensified, which was explained in terms of energy exchange with the mean field. Effect of global warming exhibits spatio-temporal heterogeneity with predominance over the Arctic region and in the winter season. Therefore, seasonal averaging may hide important features on sub-seasonal scales. In this study, distinct sub-seasonal response in storm track activities to winter Northern Hemispheric warming is analyzed applying cyclostationary empirical orthogonal function analysis to ERA5 data. The key findings are as follows. Change in the PST is not uniform throughout the winter; the PST shifts northward in early winter (NDJ) and intensifies in late winter (FM). In early winter, the combined effect of weakened baroclinic process to the south of the climatological PST and weakened barotropic damping to the north is responsible for the northward shift. In late winter, both processes contribute to the amplification of the PST. Further, change in baroclinic energy conversion is quantitatively dominated by eddy heat flux, whereas axial tilting of eddies is primarily responsible for change in barotropic energy conversion. A close relationship between anomalous eddy heat flux and anomalous boundary heating, which is largely determined by surface turbulent heat flux, is also demonstrated.


1987 ◽  
Vol 108 (3) ◽  
pp. 609-615 ◽  
Author(s):  
I. Papastylianou ◽  
Th. Samios

SummaryUsing data from rotation studies in which barley or woollypod vetch were included, both cut for hay and preceding barley for grain, it is shown that forage barley gave higher dry-matter yield than woollypod vetch (3·74 v. 2·92 t/ha per year). However, the latter gave feedingstuff of higher nitrogen concentration and yield (86 kg N/ha per year for vetch v. 55 kg N/ha per year for barley). Rainfall was an important factor in controlling the yield of the two forages and the comparison between them in different years and sites. Barley following woollypod vetch gave higher grain yield than when following forage barley (2·36 v. 1·91 t/ha). Rotation sequences which included woollypod vetch had higher output of nitrogen (N) than input of fertilizer N with a positive value of 44–60 kg N/ha per year. In rotations where forage barley was followed by barley for grain the N balance between output and input was 5–6 kg N/ha. Total soil N was similar in the different rotations at the end of a 7-year period.


1999 ◽  
Vol 79 (2) ◽  
pp. 277-286 ◽  
Author(s):  
P. A. Bowen ◽  
B. J. Zebarth ◽  
P. M. A. Toivonen

The effects of six rates of N fertilization (0, 125, 250, 375, 500 and 625 kg N ha−1) on the dynamics of N utilization relative to extractable inorganic N in the soil profile were determined for broccoli in three growing seasons. The amount of pre-existing extractable inorganic N in the soil was lowest for the spring planting, followed by the early-summer then late-summer plantings. During the first 2 wk after transplanting, plant dry-matter (DM) and N accumulation rates were low, and because of the mineralization of soil organic N the extractable soil inorganic N increased over that added as fertilizer, especially in the top 30 cm. From 4 wk after transplanting until harvest, DM and N accumulation in the plants was rapid and corresponded to a rapid depletion of extractable inorganic N from the soil. At high N-fertilization rates, leaf and stem DM and N accumulations at harvest were similar among the three plantings. However, the rates of accumulation in the two summer plantings were higher before and lower after inflorescence initiation than those in the spring planting. Under N treatments of 0 and 125 kg ha−1, total N in leaf tissue and the rate of leaf DM accumulation decreased while inflorescences developed. There was little extractable inorganic soil-N during inflorescence development in plots receiving no N fertilizer, yet inflorescence dry weights and N contents were ≥50 and ≥30%, respectively, of the maxima achieved with N fertilization. These results indicate that substantial N is translocated from leaves to support broccoli inflorescence growth under conditions of low soil-N availability. Key words: N translocation, N fertilizer


2004 ◽  
Vol 84 (2) ◽  
pp. 169-176 ◽  
Author(s):  
B. L. Ma ◽  
M. Li ◽  
L. M. Dwyer ◽  
G. Stewart

Little information is available comparing agronomic performance and nitrogen use efficiency (NUE) for N application methods such as foliar spray, soil application, and ear injection in maize (Zea mays L.). The objective of this study was to investigate the effects of various N application methods on total stover dry matter, grain yield, and NUE of maize hybrids using a 15N-labeling approach. A field experiment was conducted on a Dalhousie clay loam in Ottawa and a Guelph loam in Guelph for 2 yr (1999 and 2000). Three N application methods were tested on two maize hybrids, Pioneer 3893 and Pioneer 38P06 Bt. At planting, 60 kg N ha-1 as ammonium nitrate was applied to all treatments. In addition, 6.5 kg N ha-1 and 13.5 kg N ha-1 as 15N-labeled urea were applied to either foliage (Treatment I) or soil (Treatment II) at V6 and V12 stages, respectively. In Treatment III, 20 kg N ha-1 as 15N-labeled urea was injected into space between ear and husks at silking. The results showed that compared with soil N application neither foliar spray nor injection through ear affected grain yield or stover dry matter. The NUE values ranged from 12 to 76% for N fertilizer applied at V6 a nd V12 stages, or at silking for all treatments. There was no interaction of hybrid × N application methods on any variables measured with the only exception that for soil N application, grain NUE in Pioneer 38P06 Bt was significant higher than in Pioneer 3893. The difference in total N and NUE of grain and stover between soil N application and foliar N spray was inconsistent. However, NUE was substantially higher for N injection through the ear than for foliar or soil application without differential responses between the two hybrids. Nitrogen injection through the ear at silking might have altered N redistribution within the plant and improved NUE. Hence, it can potentially enhance grain protein content. Foliar N spray is not advocated for maize production in Ontario. Key words: Maize, Zea mays, nitrogen application methods, nitrogen-15, yield, nitrogen use efficiency


1991 ◽  
Vol 69 (12) ◽  
pp. 3071-3077 ◽  
Author(s):  
Francis E. Schwab ◽  
Michael D. Pitt

Simple linear and muliple regressions were used to determine the contribution of operative temperature (Te), forage, and snow depth to moose (Alces alces) selection of canopy cover types. The number of degree-hours for which Te exceeded the thermal limit at which panting is required to dissipate metabolic heat contributed significantly to selection during summer (1 June – 15 September) and late winter (16 January – 15 April). Forage explained canopy cover selection in early winter (16 November – 15 January) and contributed significantly to the best equation describing habitat selection during late winter. Snow depth contributed to habitat selection in early winter but was not related to habitat selection during late winter. During summer, moose generally selected against sites where Te exceeded 30 °C, the thermal limit requiring panting to dissipate metabolic heat. During late winter, moose also generally avoided canopy covers where Te commonly exceeded 8 °C, the temperature at which panting is required to dissipate heat. During early winter, moose apparently suffered minimal heat stress, as Te remained below 0 °C; consequently, moose selected canopy cover types that provided comparatively high forage availability.


1996 ◽  
Vol 36 (6) ◽  
pp. 695 ◽  
Author(s):  
CP Horn ◽  
CJ Birch ◽  
RC Dalal ◽  
JA Doughton

Mean protein concentrations in wheat (Triticum aestivum) on the Darling Downs of southern Queensland have fallen below 10% in recent years, preventing farmers from obtaining 'Prime Hard' status (13.0%) for their wheat crop. Two management options, for improving this situation are applications of nitrogenous fertiliser in a wheat monoculture or inclusion of a legume in rotation with wheat. Long-term trials at Warra, on the western Darling Downs, resulted in the selection of chickpea (Cicer arietinum) as a useful grain legume cash crop with potential for improvement of its nitrogen (N) fixing ability through management. This 2-year study examined the effect of sowing time and tillage practice on dry matter yield, grain yield, N accumulation and N2 fixation in chickpea and the subsequent soil N balance. There were 3 sowing times during autumn and winter of each year using conventional tillage (CT). Zero tillage (ZT) was introduced after the first crop for all sowing times. Greater total dry matter yield and grain yield (4.18-5.95 and 1.63-2.25 t/ha, respectively) resulted from sowing in autumn or early winter than from sowing in late winter (3.39-3.86 and 0.97-1.22 kg/ha, respectively). The effects of tillage practice were variable, depending on growth stage. At harvest, ZT plots produced greater total dry matter yield (4.20 t/ha) and grain yield (1.94 t/ha) than CT plots (3.01 and 1.29 t/ha, respectively), whereas at the time of maximum dry matter, yield was higher under CT for autumn sowings, and under ZT for winter sowings.


1958 ◽  
Vol 50 (1) ◽  
pp. 49-59 ◽  
Author(s):  
D. G. Morgan ◽  
G. C. Mees

1. Field trials with gibberellic acid on grass, wheat, potatoes, turnips, carrots, peas, runner beans, lettuce, celery, blackcurrants, kale and maize are described. The effects on plant growth and crop yield were determined.2. The experiments on grassland were carried out between July 1953 and June 1956, and included trials at four different centres. They all gave essentially similar results.3. 2 oz. per acre of gibberellic acid sprayed at 100 gal. per acre stimulated the growth of all components of the swards. The stimulation was most clearly seen in spring and autumn when the natural growth rate was slow. At these times gibberellic acid was able to produce a grazeable growth of grass more rapidly than nitrogenous fertilizers. The stimulation of growth was accompanied by yellowing of the grass, but recovery to a normal green colour was speeded up by applying a nitrogenous fertilizer at the same time as the gibberellic acid spray.4. The dry-matter yields at the first cut following a treatment with gibberellic acid were increased by 0·6–10·8 cwt. per acre. The crude protein yields were also increased by the treatment, but to a relatively smaller degree, and the protein content of the grass was reduced by between ½ and 2%. The contents of phosphorus, potassium, sodium, calcium, barium, magnesium, manganese, copper, aluminium and tin were not altered.


1974 ◽  
Vol 31 (7) ◽  
pp. 1195-1200 ◽  
Author(s):  
J. C. Medcof ◽  
M. L. H. Thomas

In late winter and early spring, benthic materials have been observed in patches (maximum size 400 m × 10 m) on the surface of thick fast ice. They parallel the shores of estuaries and are commonest in the southern Gulf of St. Lawrence. Materials in the patches are often in strikingly natural postures and include layers of bottom sediment and plants and animals (eelgrass, shellfish [infauna and epifauna] starfish, flounders, and sticklebacks). Apparently these are frozen to the lower surface of the ice in early winter at low tides, when ice comes in direct contact with them. When the tide rises, the ice floats and raises frozen-on materials with it. Thereafter, the floating ice is thickened by freezing of water to its lower surface, and the bottom materials are thus frozen in between two layers of ice. At the same time the ice above the frozen-in materials is thinned by ablation (sublimation and melting) at its upper surface. Eventually the benthic materials are exposed on the surface of the ice. Allied phenomena have been observed in the Bay of Fundy region. Antarctica is the only other place where this has been reported to occur regularly. These phenomena and conditions favoring their occurrence in the two areas are compared.


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