The serum complement system ? a mediator of acute pancreatitis

1975 ◽  
Vol 365 (3) ◽  
pp. 193-199 ◽  
Author(s):  
B. Seelig ◽  
V. Ehemann ◽  
C. Tschahargane ◽  
H. P. Seelig
Keyword(s):  
Acute Pancreatitis ◽  
Complement System ◽  
Serum Complement ◽  
System A

The serum complement system: a simplified laboratory exercise to measure the activity of an important component of the immune system

2008 ◽  
Vol 32 (4) ◽  
pp. 317-321 ◽  
Author(s):  
Jordan E. Inglis ◽  
Kimberly A. Radziwon ◽  
Gregory D. Maniero
Keyword(s):  
Immune System ◽  
Complement System ◽  
Blood Cells ◽  
Serum Complement ◽  
Hemolysis Assay ◽  
Laboratory Exercise ◽  
System A ◽  
Adaptive Mechanisms ◽  
Dissolved Components

The immune system is a vital physiological component that affords animals protection from disease and is composed of innate and adaptive mechanisms that rely on cellular and dissolved components. The serum complement system is a series of dissolved proteins that protect against a variety of pathogens. The activity of complement in serum can be determined by its ability to lyse red blood cells in vitro. Here, we describe a modification of a standard complement hemolysis assay that makes an interesting and informative laboratory exercise suitable for a variety of courses including physiology.

scholarly journals DEFICIENCY OF THE SIXTH COMPONENT OF COMPLEMENT IN RABBITS WITH AN INHERITED COMPLEMENT DEFECT

1966 ◽  
Vol 124 (4) ◽  
pp. 773-785 ◽  
Author(s):  
Klaus Rother ◽  
Ursula Rother ◽  
Hans J. Müller-Eberhard ◽  
Ulf R. Nilsson
Keyword(s):  
Complement System ◽  
Hemolytic Activity ◽  
Complement Deficiency ◽  
Rabbit Serum ◽  
Partial Purification ◽  
Normal Rabbit Serum ◽  
Chemically Modified ◽  
System A ◽  
Inactive Form

A strain of rabbits with an inherited complement deficiency was shown to lack the sixth component of the hemolytic complement system. A method was elaborated for the partial purification of this component from normal rabbit serum. Upon injection of partially purified rabbit C'6 into C'6-deficient animals, an antibody was obtained which specifically inhibited the hemolytic activity of C'6. The data suggest that C'6-deficient serum either lacks the C'6 molecule or contains it in a chemically modified and inactive form.

scholarly journals Longitudinal Stroke Recovery Associated With Dysregulation of Complement System—A Proteomics Pathway Analysis

2020 ◽  
Vol 11 ◽  
Author(s):  
Vinh A. Nguyen ◽  
Nina Riddell ◽  
Sheila G. Crewther ◽  
Pierre Faou ◽  
Harinda Rajapaksha ◽  
...  
Keyword(s):  
Pathway Analysis ◽  
Complement System ◽  
Stroke Recovery ◽  
System A

scholarly journals Vector borne pathogens and host complement system: a tangled tale

2014 ◽  
Vol 7 (Suppl 1) ◽  
pp. O9
Author(s):  
M Bhide ◽  
S Dolinska ◽  
E Bencurova
Keyword(s):  
Complement System ◽  
System A ◽  
Vector Borne

Effect of Different Sulfonamides on the Human Serum Complement System

1985 ◽  
Vol 76 (3) ◽  
pp. 205-213 ◽  
Author(s):  
I. von Zabern ◽  
R. Nolte ◽  
H. Przyklenk ◽  
W. Vogt
Keyword(s):  
Human Serum ◽  
Complement System ◽  
Serum Complement

scholarly journals Phenotypic Heterogeneity Enables Uropathogenic Escherichia coli To Evade Killing by Antibiotics and Serum Complement

2015 ◽  
Vol 83 (3) ◽  
pp. 1056-1067 ◽  
Author(s):  
Marta Putrinš ◽  
Karin Kogermann ◽  
Eliisa Lukk ◽  
Markus Lippus ◽  
Vallo Varik ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stationary Phase ◽  
Complement System ◽  
Phenotypic Heterogeneity ◽  
Serum Complement ◽  
Complement Protein ◽  
Reporter Protein ◽  
Upec Strain ◽  
Content Type ◽  
Tract Infections

Uropathogenic strains ofEscherichia coli(UPEC) are the major cause of bacteremic urinary tract infections. Survival in the bloodstream is associated with different mechanisms that help to resist serum complement-mediated killing. While the phenotypic heterogeneity of bacteria has been shown to influence antibiotic tolerance, the possibility that it makes cells refractory to killing by the immune system has not been experimentally tested. In the present study we sought to determine whether the heterogeneity of bacterial cultures is relevant to bacterial targeting by the serum complement system. We monitored cell divisions in the UPEC strain CFT073 with fluorescent reporter protein. Stationary-phase cells were incubated in active or heat-inactivated human serum in the presence or absence of different antibiotics (ampicillin, norfloxacin, and amikacin), and cell division and complement protein C3 binding were measured by flow cytometry and immunofluorescence microscopy. Heterogeneity in the doubling times of CFT073 cells in serum enabled three phenotypically different subpopulations to be distinguished, all of them being recognized by the C3 component of the complement system. The population of rapidly growing cells resists serum complement-mediated lysis. The dominant subpopulation of cells with intermediate growth rate is susceptible to serum. The third population, which does not resume growth upon dilution from stationary phase, is simultaneously protected from serum complement and antibiotics.

The complement system: a pathway linking host defence and adipocyte biology

1999 ◽  
Vol 29 (8) ◽  
pp. 653-656 ◽  
Author(s):  
Esterbauer ◽  
Krempler ◽  
Oberkofler ◽  
Patsch
Keyword(s):  
Complement System ◽  
Host Defence ◽  
System A ◽  
The Complement System
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