Effect of Different Sulfonamides on the Human Serum Complement System

1985 ◽  
Vol 76 (3) ◽  
pp. 205-213 ◽  
Author(s):  
I. von Zabern ◽  
R. Nolte ◽  
H. Przyklenk ◽  
W. Vogt
Keyword(s):  
Human Serum ◽  
Complement System ◽  
Serum Complement

scholarly journals Simple Method To Distinguish between Primary and Secondary C3 Deficiencies

2003 ◽  
Vol 10 (2) ◽  
pp. 216-220
Author(s):  
Marlene Pereira de Carvalho Florido ◽  
Patrícia Ferreira de Paula ◽  
Lourdes Isaac
Keyword(s):  
Human Serum ◽  
Complement System ◽  
Factor H ◽  
Normal Human Serum ◽  
Alternative Complement Pathway ◽  
Complement Pathway ◽  
Factor B ◽  
Alternative Complement ◽  
Normal Human ◽  
The Complement System

ABSTRACT Due to the increasing numbers of reported clinical cases of complement deficiency in medical centers, clinicians are now more aware of the role of the complement system in the protection against infections caused by microorganisms. Therefore, clinical laboratories are now prepared to perform a number of diagnostic tests of the complement system other than the standard 50% hemolytic component assay. Deficiencies of alternative complement pathway proteins are related to severe and recurrent infections; and the application of easy, reliable, and low-cost methods for their detection and distinction are always welcome, notably in developing countries. When activation of the alternative complement pathway is evaluated in hemolytic agarose plates, some but not all human sera cross-react to form a late linear lysis. Since the formation of this linear lysis is dependent on C3 and factor B, it is possible to use late linear lysis to routinely screen for the presence of deficiencies of alternative human complement pathway proteins such as factor B. Furthermore, since linear lysis is observed between normal human serum and primary C3-deficient serum but not between normal human serum and secondary C3-deficient serum caused by the lack of factor H or factor I, this assay may also be used to discriminate between primary and secondary C3 deficiencies.

Effect of Metrizamide, a Nonionic Radiographic Contrast Agent, on Human Serum Complement

1984 ◽  
Vol 73 (4) ◽  
pp. 321-329 ◽  
Author(s):  
I. von Zabern ◽  
H. Przyklenk ◽  
R. Nolte ◽  
W. Vogt
Keyword(s):  
Contrast Agent ◽  
Human Serum ◽  
Serum Complement ◽  
Radiographic Contrast

scholarly journals Phenotypic Heterogeneity Enables Uropathogenic Escherichia coli To Evade Killing by Antibiotics and Serum Complement

2015 ◽  
Vol 83 (3) ◽  
pp. 1056-1067 ◽  
Author(s):  
Marta Putrinš ◽  
Karin Kogermann ◽  
Eliisa Lukk ◽  
Markus Lippus ◽  
Vallo Varik ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stationary Phase ◽  
Complement System ◽  
Phenotypic Heterogeneity ◽  
Serum Complement ◽  
Complement Protein ◽  
Reporter Protein ◽  
Upec Strain ◽  
Content Type ◽  
Tract Infections

Uropathogenic strains ofEscherichia coli(UPEC) are the major cause of bacteremic urinary tract infections. Survival in the bloodstream is associated with different mechanisms that help to resist serum complement-mediated killing. While the phenotypic heterogeneity of bacteria has been shown to influence antibiotic tolerance, the possibility that it makes cells refractory to killing by the immune system has not been experimentally tested. In the present study we sought to determine whether the heterogeneity of bacterial cultures is relevant to bacterial targeting by the serum complement system. We monitored cell divisions in the UPEC strain CFT073 with fluorescent reporter protein. Stationary-phase cells were incubated in active or heat-inactivated human serum in the presence or absence of different antibiotics (ampicillin, norfloxacin, and amikacin), and cell division and complement protein C3 binding were measured by flow cytometry and immunofluorescence microscopy. Heterogeneity in the doubling times of CFT073 cells in serum enabled three phenotypically different subpopulations to be distinguished, all of them being recognized by the C3 component of the complement system. The population of rapidly growing cells resists serum complement-mediated lysis. The dominant subpopulation of cells with intermediate growth rate is susceptible to serum. The third population, which does not resume growth upon dilution from stationary phase, is simultaneously protected from serum complement and antibiotics.

The mechanisms of activation of normal human serum complement byEscherichia coli strains with K1 surface antigen

2006 ◽  
Vol 51 (6) ◽  
pp. 627-632 ◽  
Author(s):  
G. Bugla-Płoskońska ◽  
A. Cisowska ◽  
K. Karpińska ◽  
S. Jankowski ◽  
W. Doroszkiewicz
Keyword(s):  
Human Serum ◽  
Surface Antigen ◽  
Normal Human Serum ◽  
Serum Complement ◽  
Normal Human ◽  
Byescherichia Coli
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