scholarly journals Effect of phentolamine and preperfusion with glucose on insulin release from the isolated perfused pancreas from fasted and fed rats

Diabetologia ◽  
1975 ◽  
Vol 11 (5) ◽  
pp. 407-410 ◽  
Author(s):  
S. Efendić ◽  
E. Cerasi ◽  
R. Luft
Keyword(s):  
Insulin Release ◽  
Perfused Pancreas ◽  
Isolated Perfused Pancreas

Effect of galanin on glucose-, arginine-, or potassium-stimulated insulin release

1989 ◽  
Vol 256 (5) ◽  
pp. E619-E623
Author(s):  
T. Yoshimura ◽  
J. Ishizuka ◽  
G. H. Greeley ◽  
J. C. Thompson
Keyword(s):  
Insulin Release ◽  
High Glucose ◽  
Second Phase ◽  
Dependent Manner ◽  
Perfused Pancreas ◽  
Isolated Perfused Pancreas ◽  
Second Phases ◽  
High Concentrations ◽  
Dose Dependent ◽  
Stimulation Of

We have examined the effect of galanin infusion on glucose-stimulated release of insulin from the isolated perfused pancreas of the rat to better characterize the effect of galanin on the first and second phases of insulin release. The effects of galanin on insulin release stimulated by L-arginine or high concentrations of potassium were also examined. When perfusion of galanin was started 4 min before the start of perfusion of high glucose (16.7 mM), galanin (10(-8)-10(-11) M) inhibited both the first and second phases of insulin release in a dose-dependent manner. When perfusion of galanin (10(-8) or 10(-9) M) was started simultaneously with high glucose (16.7 mM), only the second phase of insulin release was suppressed (P less than 0.05). Galanin (10(-9) M) failed to inhibit insulin release stimulated by L-arginine (10 and 5 mM) or potassium (25 and 20 mM). These findings suggest that the inhibitory action of galanin on glucose-stimulated insulin release is exerted on early intracellular events that occur during the stimulation of insulin release and that are common to both phases. Because galanin does not inhibit insulin release stimulated by L-arginine or potassium, galanin may inhibit glucose-stimulated closure of potassium channels.

Interleukin-1 potentiates glucose stimulated insulin release in the isolated perfused pancreas

1988 ◽  
Vol 117 (3) ◽  
pp. 302-306 ◽  
Author(s):  
Lise D. Wogensen ◽  
Thomas Mandrup-Poulsen ◽  
Helle Markholst ◽  
Jens Mølvig ◽  
Åke Lernmark ◽  
...  
Keyword(s):  
Insulin Release ◽  
Cell Function ◽  
Interleukin 1 ◽  
Systemic Circulation ◽  
Second Phase ◽  
Interleukin 1 Beta ◽  
Perfused Pancreas ◽  
Porcine Pancreas ◽  
Isolated Perfused Pancreas

Abstract. The acute effects of recombinant human interleukin-1 beta (rIL-1) on basal and glucose-stimulated insulin release were investigated in the isolated perfused pancreas. At a concentration of 20 μg/l rIL-1 had no effect on basal insulin release, but increased the total amount of insulin released during first and second phase insulin release in response to 20 mmol/l D-glucose in the rat pancreas (P < 0.05). In addition, 26 μg/l of rIL-1 potentiated insulin release in response to square wave infusions of stimulatory concentrations of glucose (11 mmol/l) in the porcine pancreas. We hypothesize that IL-1 in the systemic circulation may affect B cell function in vivo.

Effects of Substance P and Substance P-(6–ll) on Hormone Release from Isolated Perfused Pancreas: Their Opposite Actions on Rat and Canine Islets*

Endocrinology ◽  
1985 ◽  
Vol 117 (5) ◽  
pp. 1996-2000 ◽  
Author(s):  
YUKINOBU CHIBA ◽  
KOICHI KAWAI ◽  
YUKICHI OKUDA ◽  
EISUKE MUNEKATA ◽  
KAMEJIRO YAMASHITA
Keyword(s):  
Substance P ◽  
Hormone Release ◽  
Perfused Pancreas ◽  
Isolated Perfused Pancreas

Interaction of gastric inhibitory polypeptide and arginine with glucose in the perfused pancreas of rats treated with triiodothyronine

1982 ◽  
Vol 60 (3) ◽  
pp. 297-301 ◽  
Author(s):  
Michael K. Mueller ◽  
Raymond A. Pederson ◽  
John C. Brown
Keyword(s):  
Insulin Secretion ◽  
Insulin Release ◽  
Chronic Treatment ◽  
Gastric Inhibitory Polypeptide ◽  
Second Phase ◽  
Perfused Pancreas ◽  
Isolated Pancreas ◽  
Insulinotropic Effect

Chronic treatment of rats with triiodothyronine (T3) resulted in suppression of insulin release from the isolated pancreas when perfused with 8.6 mM glucose. This inhibition could be partially overcome by 16 mM glucose but the insulin release was still significantly reduced. Arginine and gastric inhibitory polypeptide (GIP) induced an insulinotropic action in both control and T3-treated preparations. This was achieved in the latter, in the absence of a second phase of insulin secretion to glucose. The insulinotropic effect of both arginine and GIP was abolished by mannoheptulose in both control and T3-treated animals.

α- and β-Cell Function in Obese Zucker (fa/fa) Rats: A Study with the Isolated Perfused Pancreas

1994 ◽  
Vol 86 (3) ◽  
pp. 311-316 ◽  
Author(s):  
Dr Hiroshi Hirose ◽  
Hiroshi Maruyama ◽  
Koichi Kido ◽  
Katsuhiko Ito ◽  
Kazunori Koyama ◽  
...  
Keyword(s):  
Glucose Concentration ◽  
Cell Function ◽  
Glucagon Secretion ◽  
Zucker Rats ◽  
Perfused Pancreas ◽  
Isolated Perfused Pancreas ◽  
Insulin And Glucagon Secretion ◽  
Β Cell Function ◽  
Insulin Secretion Rate ◽  
Insulin Secretory Response

1. The effects of various stimuli, including changes in glucose concentration, arginine, tyramine and noradrenaline, on insulin and glucagon secretion were investigated using isolated perfused pancreata of obese and lean male Zucker rats at 12 months of age. 2. In Zucker fatty rats, the insulin secretion rate was significantly (P < 0.01) higher than that of lean rats at all glucose concentrations tested (8.3, 16.7 and 1.4 mmol/l). However, the integrated insulin secretory response to raising the glucose concentration from 8.3 to 16.7 mmol/l was almost absent in these rats. The glucagon secretion rates were significantly lower at 8.3 and 1.4 mmol/l glucose (P < 0.001 for both), and in responses to 10 μg/ml tyramine and 0.1 μmol/l noradrenaline (P < 0.05 for both), in Zucker fatty rats. Integrated insulin and glucagon responses to 10 mmol/l arginine were identical in the two groups. 3. Histopathological and immunochemical studies revealed hyperplasia of β-cells and scattered α-cells in the enlarged islets of Zucker fatty rats. 4. These results suggest that, in Zucker fatty rats, the decreased glucagon secretion in the isolated perfused pancreas is attributable to changes in the environment of α-cells and/or the inhibitory effects of hypersecreted insulin.

The physiology of the isolated perfused pancreas

Cryobiology ◽  
1984 ◽  
Vol 21 (6) ◽  
pp. 688
Author(s):  
L.-H. Toledo-Pereyra
Keyword(s):  
Perfused Pancreas ◽  
Isolated Perfused Pancreas
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