Differential inhibitory effects of noradrenaline upon insulin secretion by the isolated perfused pancreas of the rat

1982 ◽  
Vol 38 (9) ◽  
pp. 1116-1118 ◽  
Author(s):  
R. J. Howland ◽  
G. M. Baroody
Keyword(s):  
Insulin Secretion ◽  
Perfused Pancreas ◽  
Inhibitory Effects ◽  
Isolated Perfused Pancreas

INSULIN SECRETION OF THE ISOLATED PERFUSED PANCREAS OF THE CHINESE HAMSTER (CRICETULUS GRISEUS)

1974 ◽  
Vol 76 (2) ◽  
pp. 302-318 ◽  
Author(s):  
E. Gerhards ◽  
M. Rühl
Keyword(s):  
Insulin Secretion ◽  
Chinese Hamster ◽  
Cricetulus Griseus ◽  
Perfused Pancreas ◽  
Short Term ◽  
Isolated Perfused Pancreas ◽  
Perfusion Studies ◽  
Dynamics Of Insulin Secretion ◽  
Linear Manner ◽  
Second Peak

ABSTRACT The insulin secretion pattern of the isolated perfused pancreas of the non-diabetic Chinese hamster after stimulation with glucose and tolbutamide is described. Stimulation with 100–800 mg glucose/100 ml leads to an increasing acute insulin secretion. The initial insulin peak reaches its maximum 2–3 min after the beginning of the stimulation with glucose. The amount of insulin initially secreted from the pancreas of fasted hamsters is larger than that of fed animals. The initial insulin peak is followed by a second short-term insulin release. The maximum of the second peak occurs approximately 10–15 min after the initial stimulation with glucose. With 100 mg glucose/100 ml the insulin secretion proceeds in a practically linear manner for a period of 140 min. With 200 and 400 mg glucose/100 ml the insulin secretion remains linear for approximately 80 min. With 200 mg glucose/100 ml about 10 000 μU insulin are secreted within 120 min, with 400 mg glucose/100 ml approximately 20 000 μU are secreted in the same period of time. The insulin secretion is described following repeated short-term stimulation with 200 mg glucose/100 ml and increasing glucose concentrations up to 500 mg/100 ml for 5 min each. In perfusion studies with 400 mg glucose/100 ml and 8 mg puromycin/100 ml the amount of insulin secreted in 150 min is reduced by about 50%. The insulin secretion stimulated by 100 mg glucose/100 ml was greatly increased by 10 mg tolbutamide/100 ml. The insulin secretion pattern at a glucose concentration of 50 mg/100 ml is shown following repeated stimulation with 10 mg tolbutamide/100 ml alternating with tolbutamide-free resting phases of 10 min. The mechanism of insulin secretion following stimulation with glucose and β-cytotrophic antidiabetics is discussed. The isolated perfused pancreas of the Chinese hamster is suitable for the study of the dynamics of insulin secretion.

Alterations in pulsatile insulin secretion in the Zucker diabetic fatty rat

1994 ◽  
Vol 267 (2) ◽  
pp. E250-E259 ◽  
Author(s):  
J. Sturis ◽  
W. L. Pugh ◽  
J. Tang ◽  
D. M. Ostrega ◽  
J. S. Polonsky ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Spectral Power ◽  
Pulse Amplitude ◽  
Ringer Solution ◽  
Cellular Level ◽  
Diabetic Rats ◽  
Male Rats ◽  
Perfused Pancreas ◽  
Isolated Perfused Pancreas ◽  
Pulsatile Insulin Secretion

Insulin secretion from the isolated perfused pancreas is characterized by pulses occurring every 5-15 min. The present experiments were performed to explore the role of glucose in regulating these pulses. The pancreata from 12 Wistar (W), 12 Zucker diabetic fatty (ZDF), and 6 nondiabetic lean Zucker control (ZC) male rats were isolated and perfused at 37 degrees C with an oxygenated Krebs Ringer solution containing bovine serum albumin and glucose. In W and ZDF, insulin secretion was pulsatile during constant glucose, as assessed by pulse analysis (ULTRA). The pulse period in W was significantly shorter than in ZDF (7.1 +/- 0.6 vs. 14.7 +/- 1.0 min; P < 0.001), whereas the median relative pulse amplitude was not different. When glucose was administered as a series of 10-min sine waves, spectral analysis showed that the normalized spectral power at 10 min was greater in W and ZC compared with ZDF (34.2 +/- 5.9 and 32.9 +/- 2.9 vs. 3.2 +/- 0.9; P < 0.0001), demonstrating entrainment of the insulin pulses to the exogenous glucose oscillations in W and ZC but not in ZDF. Furthermore, in ZDF, the insulin secretory rates were not higher when 28 mM rather than 7 mM glucose were used. In additional studies, islets of Langerhans from one W, three ZDF, and three ZC rats were isolated and perifused using an oscillatory glucose concentration. Single and groups of islets were studied. Islets from diabetic rats demonstrated the same lack of entrainment by glucose seen in the perfused pancreas, suggesting that the defect is at the cellular level.(ABSTRACT TRUNCATED AT 250 WORDS)

Inhibition of Insulin Secretion by Diphenylhydantoin in the Isolated Perfused Pancreas

1970 ◽  
Vol 30 (3) ◽  
pp. 400-401 ◽  
Author(s):  
SEYMOUR R. LEVIN ◽  
JOSEPH BOOKER ◽  
DESMOND F. SMITH ◽  
GEROLD M. GRODSKY
Keyword(s):  
Insulin Secretion ◽  
Perfused Pancreas ◽  
Isolated Perfused Pancreas

Somatostatin, glucagon, and insulin secretion from isolated perfused pancreas of new genetically obese hyperglycemic rats

Metabolism ◽  
1984 ◽  
Vol 33 (5) ◽  
pp. 429-431 ◽  
Author(s):  
Yutaka Seino ◽  
Susumu Seino ◽  
Masaru Usami ◽  
Kinsuke Tsuda ◽  
Jiro Takemura ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Perfused Pancreas ◽  
Isolated Perfused Pancreas ◽  
Glucagon And Insulin

Preservation of insulin secretory responses to P2 purinoceptor agonists in Zucker diabetic fatty rats

1996 ◽  
Vol 270 (3) ◽  
pp. E504-E512 ◽  
Author(s):  
J. Tang ◽  
W. Pugh ◽  
K. S. Polonsky ◽  
H. Zhang
Keyword(s):  
Insulin Secretion ◽  
Insulin Dependent Diabetes Mellitus ◽  
Secretory Process ◽  
Dependent Diabetes Mellitus ◽  
Perfused Pancreas ◽  
Isolated Perfused Pancreas ◽  
Zucker Diabetic Fatty Rats ◽  
Voltage Dependent ◽  
Insulin Dependent ◽  
Zdf Rats

The role of P2 purinoceptor agonists in regulatory insulin secretion in Zucker diabetic fatty (ZDF) rats was studied using the isolated perfused pancreas and intracellular Ca2+ concentration ([Ca2+]i) microfluorimetry. The relative potency of different purinoceptor agonists to stimulate the insulin secretory process was consistent with the conclusion that responses in [Ca2+]i and insulin secretion are mediated by the P2y subtype of purinoceptors. Additional studies using specific antagonists of the Ca2+ signaling pathway indicated that activation of P2y purinoceptor releases Ca2+ from intracellular stores and promotes Ca2+ entry through voltage-independent rather than voltage-dependent Ca2+ channels on the beta-cell membrane. Perfused pancreas and isolated islets from ZDF rats demonstrated markedly reduced or absent insulin secretion and [Ca2+]i responses to glucose and KCl. In contrast, responses to P2y purinoceptor agonists were normal, indicating that the secretion coupling pathway activated by these agonists is preserved in glucose-unresponsive islets from diabetic animals. These observations raise the possibility that the purinoceptor pathway may play an important role in regulating insulin secretion in hyperinsulinemic non-insulin-dependent diabetes mellitus.

Biotin enhances glucose-stimulated insulin secretion in the isolated perfused pancreas of the rat

1999 ◽  
Vol 10 (4) ◽  
pp. 237-243 ◽  
Author(s):  
Hideyuki Sone ◽  
Michiko Ito ◽  
Kimihiko Sugiyama ◽  
Makoto Ohneda ◽  
Masaru Maebashi ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Perfused Pancreas ◽  
Isolated Perfused Pancreas ◽  
Glucose Stimulated Insulin Secretion
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