Kinetics of bacteriophage λ repressor synthesis directed by the P RE promoter: Influence of temperature, multiplicity of infection, and mutation of P RM or the cro gene

1980 ◽  
Vol 179 (2) ◽  
pp. 409-419 ◽  
Author(s):  
Kwang-Mu Yen ◽  
Gary N. Gussin
2013 ◽  
Vol 634-638 ◽  
pp. 541-545 ◽  
Author(s):  
Jun Seong Park ◽  
Dae Hee Yun ◽  
Tae Won Ko ◽  
Yong Sung Park ◽  
Je Wan Woo

The kinetics of the Diels-Alder reaction of cyclopentadiene with bis(2-ethylhexyl) maleate has been studied at temperatures between 25 and 100 °C and at atmospheric pressure. The influence of temperature on the kinetic constants was determined by fitting the results to the Arrhenius equation. As a result, fitting line similar with the linear curve of the Arrhenius equation at 25, 30 and 40 °C. However, the fitting curve, at 60, 80 and 100 °C, tended towards the outside of the curve in the form of Arrhenius equation. The ratio of endo/exo was a slight change from increase of the reaction temperature.


2014 ◽  
Vol 955-959 ◽  
pp. 2306-2309
Author(s):  
Shu Bin Zhao ◽  
Pan Zhang ◽  
Xiao Tong Cai ◽  
Dong Fu

The absorption rates of CO2 in diethanolamine (DEA) promoted N-methyldiethanolamine (MDEA) aqueous solution were measured at normal pressure with temperatures ranging from 303.15-323.15K. The influence of temperature and the mass fraction of DEA on the absorption rate of CO2 was illustrated.


1998 ◽  
Vol 54 (3-4) ◽  
pp. 131-145 ◽  
Author(s):  
Fenwei Su ◽  
K Hanumantha Rao ◽  
K.S.E Forssberg ◽  
P.O Samskog

2015 ◽  
Vol 228 ◽  
pp. 237-241
Author(s):  
Magdalena Popczyk ◽  
B. Łosiewicz

The Ni+MoS2composite coatings were prepared by electrodeposition under galvanostatic conditions from the Ni-plating bath containing suspended MoS2powder (100 mesh). Investigations of hydrogen evolution reaction (HER) were carried out using steady-state polarization measurements and electrochemical impedance spectroscopy (EIS) in 5 M KOH solution on the coatings obtained at 30, 40, and 50°C. It was found that the kinetics of the HER on the Ni+MoS2coatings decreases with the increase in the electrodeposition temperature of the coatings. This effect is attributed to decreasing content of MoS2(from 26.4 to 18.0 wt.%) embedded into the Ni matrix as composite crystalline component having the electrocatalytic properties towards the HER and/or surface development of the coatings. The higher amount of MoS2was embedded, the more porous electrodes containing pear-shape pores on the surface were produced what was detected by EIS.


2018 ◽  
Vol 509 ◽  
pp. 488-495 ◽  
Author(s):  
Yulia Arinicheva ◽  
Clemence Gausse ◽  
Stefan Neumeier ◽  
Felix Brandt ◽  
Konstantin Rozov ◽  
...  

2012 ◽  
Vol 31 (1) ◽  
Author(s):  
Michal Besterci ◽  
Marián Varchola ◽  
Ladislav Kováč ◽  
Oksana Velgosová

AbstractThe influence of temperature at constant strain rate has been evaluated with respect to superplastic behavior of dispersion strengthened Al-Al


2009 ◽  
Vol 83 (15) ◽  
pp. 7487-7494 ◽  
Author(s):  
Pablo González-Jara ◽  
Aurora Fraile ◽  
Tomás Canto ◽  
Fernando García-Arenal

ABSTRACT The multiplicity of infection (MOI), i.e., the number of virus genomes that infect a cell, is a key parameter in virus evolution, as it determines processes such as genetic exchange among genomes, selection intensity on viral genes, epistatic interactions, and the evolution of multipartite viruses. In fact, the MOI level is equivalent to the virus ploidy during genome expression. Nevertheless, there are few experimental estimates of MOI, particularly for viruses with eukaryotic hosts. Here we estimate the MOI of Tobacco mosaic virus (TMV) in its systemic host, Nicotiana benthamiana. The progress of infection of two TMV genotypes, differently tagged with the green or red fluorescent proteins GFP and RFP, was monitored by determining the number of leaf cell protoplasts that showed GFP, RFP, or GFP and RFP fluorescence at different times postinoculation. This approach allowed the quantitative analysis of the kinetics of infection and estimation of the generation time and the number of infection cycles required for leaf colonization. MOI levels were estimated from the frequency of cells infected by only TMV-GFP or TMV-RFP. The MOI was high, but it changed during the infection process, decreasing from an initial level of about 6 to a final one of 1 to 2, with most infection cycles occurring at the higher MOI levels. The decreasing MOI can be explained by mechanisms limiting superinfection and/or by genotype competition within double-infected cells, which was shown to occur in coinfected tobacco protoplasts. To our knowledge, this is the first estimate of MOI during virus colonization of a eukaryotic host.


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