Biosynthesis of bacterial glycogen: characterization of adenosine diphosphate glucose synthetases from Enterobacter hafniae and Aeromonas hydrophila

1984 ◽  
Vol 138 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Sau-Gee Yung ◽  
Marvin Paule ◽  
Russell Beggs ◽  
Elaine Greenberg ◽  
Jack Preiss
Keyword(s):  
Aeromonas Hydrophila ◽  
Adenosine Diphosphate ◽  
Diphosphate Glucose

scholarly journals Purification and Characterization of Adenosine Diphosphate Glucose Pyrophosphorylase from Maize/Potato Mosaics

2005 ◽  
Vol 138 (3) ◽  
pp. 1552-1562 ◽  
Author(s):  
Susan K. Boehlein ◽  
Aileen K. Sewell ◽  
Joanna Cross ◽  
Jon D. Stewart ◽  
L. Curtis Hannah
Keyword(s):  
Adenosine Diphosphate ◽  
Purification And Characterization ◽  
Diphosphate Glucose

scholarly journals Molecular and Functional Characterization of a ToxR-Regulated Lipoprotein from a Clinical Isolate of Aeromonas hydrophila

2006 ◽  
Vol 74 (7) ◽  
pp. 3742-3755 ◽  
Author(s):  
Lakshmi Pillai ◽  
Jian Sha ◽  
Tatiana E. Erova ◽  
Amin A. Fadl ◽  
Bijay K. Khajanchi ◽  
...  
Keyword(s):  
Virulence Factor ◽  
Aeromonas Hydrophila ◽  
Functional Characterization ◽  
Affinity Column ◽  
Serum Resistance ◽  
Classical Pathway ◽  
Dependent Manner ◽  
T7 Promoter

ABSTRACT Human diseases caused by species of Aeromonas have been classified into two major groups: septicemia and gastroenteritis. In this study, we reported the molecular and functional characterization of a new virulence factor, ToxR-regulated lipoprotein, or TagA, from a diarrheal isolate, SSU, of Aeromonas hydrophila. The tagA gene of A. hydrophila exhibited 60% identity with that of a recently identified stcE gene from Escherichia coli O157:H7, which encoded a protein (StcE) that provided serum resistance to the bacterium and prevented erythrocyte lysis by controlling classical pathway of complement activation by cleaving the complement C1-esterase inhibitor (C1-INH). We purified A. hydrophila TagA as a histidine-tagged fusion protein (rTagA) from E. coli DE3 strain using a T7 promoter-based pET30 expression vector and nickel affinity column chromatography. rTagA cleaved C1-INH in a time-dependent manner. The tagA isogenic mutant of A. hydrophila, unlike its corresponding wild-type (WT) or the complemented strain, was unable to cleave C1-INH, which is required to potentiate the C1-INH-mediated lysis of host and bacterial cells. We indeed demonstrated colocalization of C1-INH and TagA on the bacterial surface by confocal fluorescence microscopy, which ultimately resulted in increased serum resistance of the WT bacterium. Likewise, we delineated the role of TagA in contributing to the enhanced ability of C1-INH to inhibit the classical complement-mediated lysis of erythrocytes. Importantly, we provided evidence that the tagA mutant was significantly less virulent in a mouse model of infection (60%) than the WT bacterium at two 50% lethal doses, which resulted in 100% mortality within 48 h. Taken together, our data provided new information on the role of TagA as a virulence factor in bacterial pathogenesis. This is the first report of TagA characterization from any species of Aeromonas.

scholarly journals Kinetic and spectroscopic characterization of native and metal-substituted β-lactamase from Aeromonas hydrophila AE036

FEBS Letters ◽  
2000 ◽  
Vol 467 (2-3) ◽  
pp. 221-225 ◽  
Author(s):  
Maria Hernandez Valladares ◽  
Martin Kiefer ◽  
Uwe Heinz ◽  
Raquel Paul Soto ◽  
Wolfram Meyer-Klaucke ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Spectroscopic Characterization

Characterization of the ceruloplasmin gene and its potential role as an indirect marker for selection to Aeromonas hydrophila resistance in rohu, Labeo rohita

2013 ◽  
Vol 34 (5) ◽  
pp. 1325-1334 ◽  
Author(s):  
P.K. Sahoo ◽  
Sweta Das ◽  
Kanta Das Mahapatra ◽  
Jatindra Nath Saha ◽  
Matthew Baranski ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Potential Role ◽  
Labeo Rohita
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