Titrimetric determination of iron(II), molybdenum(V) and thallium(I) with potassium chlorate to a potentiometric end-point

1971 ◽  
Vol 254 (5) ◽  
pp. 366-367 ◽  
Author(s):  
C. Radhakrishnamurty ◽  
G. Gopala Rao
Keyword(s):  
Potassium Chlorate ◽  
End Point ◽  
Titrimetric Determination

Titrimetric determination of uranium with fluorimetric end-point detection

1971 ◽  
Vol 57 (1) ◽  
pp. 159-163 ◽  
Author(s):  
F.H.P. Koning ◽  
G. Den Boef ◽  
H. Poppe
Keyword(s):  
End Point ◽  
Titrimetric Determination ◽  
End Point Detection ◽  
Point Detection

scholarly journals KD determination from time-resolved experiments on live cells with LigandTracer and reconciliation with end-point flow cytometry measurements

2021 ◽  
Author(s):  
Diana Spiegelberg ◽  
Jonas Stenberg ◽  
Pascale Richalet ◽  
Marc Vanhove
Keyword(s):  
Flow Cytometry ◽  
Live Cells ◽  
Time Resolved ◽  
Surface Receptors ◽  
Full Characterization ◽  
End Point ◽  
Titration Curves ◽  
Kinetics Of

AbstractDesign of next-generation therapeutics comes with new challenges and emulates technology and methods to meet them. Characterizing the binding of either natural ligands or therapeutic proteins to cell-surface receptors, for which relevant recombinant versions may not exist, represents one of these challenges. Here we report the characterization of the interaction of five different antibody therapeutics (Trastuzumab, Rituximab, Panitumumab, Pertuzumab, and Cetuximab) with their cognate target receptors using LigandTracer. The method offers the advantage of being performed on live cells, alleviating the need for a recombinant source of the receptor. Furthermore, time-resolved measurements, in addition to allowing the determination of the affinity of the studied drug to its target, give access to the binding kinetics thereby providing a full characterization of the system. In this study, we also compared time-resolved LigandTracer data with end-point KD determination from flow cytometry experiments and hypothesize that discrepancies between these two approaches, when they exist, generally come from flow cytometry titration curves being acquired prior to full equilibration of the system. Our data, however, show that knowledge of the kinetics of the interaction allows to reconcile the data obtained by flow cytometry and LigandTracer and demonstrate the complementarity of these two methods.

scholarly journals TITRIMETRIC DETERMINATION OF TOTAL ALDEHYDES IN CINNAMON BARK AND LEAF OILS USING CHLORAMINE-T REAGENT

1981 ◽  
Vol 4 (1) ◽  
pp. 35-41 ◽  
Author(s):  
M. L. SHANKARANARAYANA ◽  
B. RAGHAVAN ◽  
K. O. ABRAHAM ◽  
C. P. NATARAJAN
Keyword(s):  
Cinnamon Bark ◽  
Titrimetric Determination ◽  
Chloramine T
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