Killer toxin producing strains of the yeasts Hanseniaspora uvarum and Pichia kluyveri

1988 ◽  
Vol 149 (3) ◽  
pp. 261-267 ◽  
Author(s):  
J. Zorg ◽  
S. Kilian ◽  
F. Radler
Keyword(s):  
Killer Toxin ◽  
Hanseniaspora Uvarum ◽  
Pichia Kluyveri

scholarly journals Dynamic behavior of Saccharomyces cerevisiae, Pichia kluyveri and Hanseniaspora uvarum during spontaneous and inoculated cocoa fermentations and their effect on sensory characteristics of chocolate

LWT ◽  
2015 ◽  
Vol 63 (1) ◽  
pp. 221-227 ◽  
Author(s):  
Nadia Nara Batista ◽  
Cíntia Lacerda Ramos ◽  
Disney Dias Ribeiro ◽  
Ana Carla Marques Pinheiro ◽  
Rosane Freitas Schwan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dynamic Behavior ◽  
Sensory Characteristics ◽  
Hanseniaspora Uvarum ◽  
Pichia Kluyveri

Effects of Pichia kluyveri killer toxin on sensitive cells

1980 ◽  
Vol 46 (2) ◽  
pp. 205-220 ◽  
Author(s):  
E. J. Middelbeek ◽  
C. Stumm ◽  
G. D. Vogels
Keyword(s):  
Killer Toxin ◽  
Pichia Kluyveri

The incidence of killer activity and extracellular proteases in tropical yeast communities

1997 ◽  
Vol 43 (4) ◽  
pp. 328-336 ◽  
Author(s):  
Jacqueline Abranches ◽  
Leda C. Mendonça-Hagler ◽  
Allen N. Hagler ◽  
Paula B. Morais ◽  
Carlos A. Rosa
Keyword(s):  
Killer Toxin ◽  
Spatial Separation ◽  
Extracellular Proteases ◽  
Temporal Separation ◽  
Killer Activity ◽  
Killer Yeasts ◽  
Sporobolomyces Roseus ◽  
Nitrogen Nutrients ◽  
Pichia Kluyveri ◽  
Ascomycetous Yeasts

The presence of killer and proteolytic yeasts was studied among 944 isolates representing 105 species from tropical yeast communities. We found 13 killer toxin producing species, with Pichia kluyveri being the most frequent. Other killer yeast isolates were Candida apis, Candida bombicola, Candida fructus, Candida krusei, Candida sorbosa, Hanseniaspora uvarum, Issatchenkia occidentalis, Kloeckera apis, Kluyveromyces marxianus, Pichia membranaefaciens, Pichia ohmeri-like, and Sporobolomyces roseus. The communities from which killer yeasts were isolated had strains sensitive to them, and there were interspecific and intraspecific differences in the spectra of their killer activities. Pichia kluyveri had the broadest spectra of activity against sensitive isolates, and it apparently produced different toxins. The coexistence of sensitive and killer yeasts using the same substrate suggests that there is spatial separation in microhabitats or temporal separation in different stages of successions. Basidiomycetous yeasts were more frequently proteolytic than ascomycetous yeasts. Extracellular proteases could be important for the yeasts to have access to more nitrogen nutrients and obtain a better balance with available carbon sources.Key words: killer yeasts, extracellular proteases, tropical yeast communities.

Yeasts and yeast-like organisms associated with fruits and blossoms of different fruit trees

2012 ◽  
Vol 58 (12) ◽  
pp. 1344-1352 ◽  
Author(s):  
Renáta Vadkertiová ◽  
Jana Molnárová ◽  
Dana Vránová ◽  
Elena Sláviková
Keyword(s):  
Aureobasidium Pullulans ◽  
Fruit Trees ◽  
Individual Species ◽  
Pichia Kudriavzevii ◽  
Hanseniaspora Uvarum ◽  
Yeast Community ◽  
Fruit Samples ◽  
Pear Trees ◽  
Pichia Kluyveri ◽  
Isolated Species

Yeasts are common inhabitants of the phyllosphere, but our knowledge of their diversity in various plant organs is still limited. This study focused on the diversity of yeasts and yeast-like organisms associated with matured fruits and fully open blossoms of apple, plum, and pear trees, during 2 consecutive years at 3 localities in southwest Slovakia. The occurrence of yeasts and yeast-like organisms in fruit samples was 2½ times higher and the yeast community more diverse than that in blossom samples. Only 2 species (Aureobasidium pullulans and Metschnikowia pulcherrima) occurred regularly in the blossom samples, whereas Galactomyces candidus, Hanseniaspora guilliermondii, Hanseniaspora uvarum, M. pulcherrima, Pichia kluyveri, Pichia kudriavzevii, and Saccharomyces cerevisiae were the most frequently isolated species from the fruit samples. The ratio of the number of samples where only individual species were present to the number of samples where 2 or more species were found (consortium) was counted. The occurrence of individual species in comparison with consortia was much higher in blossom samples than in fruit samples. In the latter, consortia predominated. Aureobasidium pullulans, M. pulcherrima, and S. cerevisiae, isolated from both the fruits and blossoms, can be considered as resident yeast species of various fruit tree species cultivated in southwest Slovakia localities.

scholarly journals Brewing and Probiotic Potential Activity of Wild Yeasts Hanseniaspora Uvarum PIT001, Pichia Kluyveri LAR001 and Candida Intermedia ORQ001

2021 ◽  
Author(s):  
Renan Eugênio Araujo Piraine ◽  
Gustavo M Retzlaf ◽  
Vitória S. Gonçalves ◽  
Rodrigo C Cunha ◽  
Fabio Pereira Leivas Leite
Keyword(s):  
Pathogenic Bacteria ◽  
Inhibitory Effect ◽  
Starter Cultures ◽  
Mixed Fermentation ◽  
Candida Intermedia ◽  
Probiotic Potential ◽  
Hanseniaspora Uvarum ◽  
Wide Range ◽  
Pichia Kluyveri

Abstract Non-conventional yeasts can be isolated from a wide range of environmental sources, often found in beverage industry in mixed fermentations, in which the microorganisms’ inoculum usually is not fully known. It is important to know starter cultures, since in addition to favoring reproducibility, other properties can be discovered. Thus, the objective of this work was to identify and characterize yeasts isolated from environment, evaluating their probiotic potential and possible use in brewery. Isolates were obtained from flowers, fruits, leaves and mixed-fermentation beers, being identified by PCR. Yeasts with promising activity were evaluated regarding their growth under different pHs, temperature and presence of organic acids. To explore probiotic potential, in vitro tests were performed of antimicrobial activity and co-aggregation with food pathogens, auto-aggregation, and survival in simulated gastrointestinal tract conditions. In our study, Pichia kluyveri (LAR001), Hanseniaspora uvarum (PIT001) and Candida intermedia (ORQ001) were selected among 20 isolates. P. kluyveri was the only one that tolerated pH 2.5. Lactic acid was not inhibitory, while acetic acid and incubation at 37 °C had a partially inhibitory effect on yeasts growth. All yeasts tolerated α-acids from hops and NaCl up to 1%. It is suggested that isolates are able to adhere to intestinal cells and influence positively the organism in combating pathogens, as they showed auto-aggregation rates above 99% and antagonistic activity to pathogenic bacteria. The yeasts tolerated gastric environment conditions, however were more sensitive to pancreatic conditions. We conclude that isolated non-conventional yeasts showed probiotic potential and promising application in beer fermentation.

scholarly journals Killer Activity of Hanseniaspora uvarum Isolated from Dates Vinegar: Partially Purification and Characterization of Killer Toxin

2019 ◽  
Vol 16 (1(Suppl.)) ◽  
pp. 0140
Author(s):  
Hameed Et al.
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Plant Pathogens ◽  
Killer Toxin ◽  
Toxin Production ◽  
Antagonistic Activity ◽  
Killer Activity ◽  
E Coli ◽  
Killer Yeast ◽  
Hanseniaspora Uvarum

This study was conducted to isolate and identify killer yeast Hanseniaspora uvarum from dates vinegar and measurement the ability of this yeast to produce killer toxin. The antimicrobial activity of the concentrated supernatant containing partially purified concentrated killer toxin was also detected against several pathogenic bacteria and yeast species, which includes two types of yeast Rhodotorula mucilaginosa and Candida tropicalis and four human pathogenic bacteria Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeurginosa. In addition, the antagonistic activity of examined yeast have been studied toward four types of fungi, where two are pathogenic for human Trichophyton mentagrophytes and Trichophyton rubrum and two are plant pathogens Fusarium solani and Sclerotinia sclerotiorum. The results of killer toxin production experiments revealed the ability of yeast to produce killer toxin with molecular weight at 18 kDa by 12 % SDS electrophoresis. The optimal conditions for killer toxin production were studied, and their antimicrobial activity was determined. The results revealed that killer toxin production was increased at 4 % NaCl, the highest inhibtion zone was 20 mm for S. aureus, while the lowest inhibition zone was 7 mm for E. coli. Killer activity was increased at pH 4 and the best inhibtion zone obtained was about 16 mm for K. pneumoniae, while 8 mm for E. coli and C. tropicalis. The temperature was also affect the production of killer toxin, where 25 °C is the best temperature for toxin production of examined yeast, The best killer activity was 21 mm for C. tropicalis. The antagonistic activity of killer yeast H. uvarum toward pathogenic fungal growth was determined and showed killer activity about 61.11, 44.44, 33.33 and 24.44 % against T. mentagrophytes, T.  rubrum, F. solani and S. sclerotiorum in comparison to the control.

scholarly journals Effects of Simultaneous Co-Fermentation of Five Indigenous Non-Saccharomyces Strains with S. cerevisiae on Vidal Icewine Aroma Quality

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1452
Author(s):  
Qian Ge ◽  
Chunfeng Guo ◽  
Jing Zhang ◽  
Yue Yan ◽  
Danqing Zhao ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pure Culture ◽  
Ethyl Esters ◽  
Hanseniaspora Uvarum ◽  
Yeast Strains ◽  
Future Production ◽  
Isobutyl Acetate ◽  
Saccharomyces Yeast ◽  
Novel Strategy ◽  
Pichia Kluyveri

In this study, Vidal grape must was fermented using commercial Saccharomyces cerevisiae F33 in pure culture as a control and in mixed culture with five indigenous non-Saccharomyces yeast strains (Hanseniaspora uvarum QTX22, Saccharomycopsis crataegensis YC30, Pichia kluyveri HSP14, Metschnikowia pulcherrima YC12, and Rhodosporidiobolus lusitaniae QTX15) through simultaneous fermentation in a 1:1 ratio. Simultaneous fermentation inhibited the growth of S. cerevisiae F33 and delayed the time to reach the maximum biomass. Compared with pure fermentation, the contents of polyphenols, acetic esters, ethyl esters, other esters, and terpenes were increased by R. lusitaniae QTX15, S. crataegensis YC30, and P. kluyveri HSP14 through simultaneous fermentation. S. crataegensis YC30 produced the highest total aroma activity and the most abundant aroma substances of all the wine samples. The odor activity values of 1 C13-norisoprenoid, 3 terpenes, 6 acetic esters, and 10 ethyl esters improved significantly, and three lactones (δ-decalactone, γ-nonalactone, and γ-decalactone) related to coconut and creamy flavor were only found in this wine. Moreover, this sample showed obvious “floral” and “fruity” note odor due to having the highest amount of ethyl ester aromatic substances and cinnamene, linalool, citronellol, β-damascenone, isoamyl ethanoate, benzylcarbinyl acetate, isobutyl acetate, etc. We suggest that simultaneous fermentation of S. crataegensis YC30 with S. cerevisiae might represent a novel strategy for the future production of Vidal icewine.

scholarly journals Immunostimulatory activity of Pichia kluyveri, Hanseniaspora uvarum, Candida intermedia, Saccharomyces boulardii and their derivatives on RAW 264.7 macrophages

2021 ◽  
Author(s):  
Renan Eugênio Araujo Piraine ◽  
Vitória Sequeira Gonçalves ◽  
Neida Lucia Conrad ◽  
Fábio Pereira Leivas Leite
Keyword(s):  
Transcription Factors ◽  
Fold Increase ◽  
Saccharomyces Boulardii ◽  
Specific Response ◽  
Raw 264.7 ◽  
Mrna Transcription ◽  
Candida Intermedia ◽  
Hanseniaspora Uvarum ◽  
Tnf Α ◽  
Pichia Kluyveri

AbstractYeasts are a group of microorganisms with structural and metabolic characteristics that influence their recognition by immune cells resulting in a species-specific response. Although Saccharomyces boulardii is a widely studied probiotic yeast, immunostimulation by non-Saccharomyces yeasts still underexplored. Therefore, the aim of this study was to characterize the response induced in macrophages stimulated by yeasts Pichia kluyveri, Hanseniaspora uvarum, Candida intermedia and their derivatives: heat-killed cells, supernatant and DNA. RAW 264.7 murine macrophages were stimulated in vitro for 24 h and the response generated was evaluated by analyzing mRNA transcription of cytokines (IL2, IL4, IL10, IL13, IL23, TNF-α), transcription factors (Bcl6, NFκβ, STAT3), Toll-like receptor 2 (TLR2) and YM1 protein. Viable and heat-killed cells of P. kluyveri and H. uvarum were responsible for high levels of relative mRNA transcription of transcription factors and TLR2 (between 2 – 8-fold increase), however were able to induce only low transcription levels for analyzed cytokines (≤ 2-fold increase). Viable cells of C. intermedia were able to stimulate a significant transcription of IL4 (7.6-fold increase) and Bcl6 (4-fold increase), while heat-killed cells stimulated the highest level of TNF-α (2.4-fold increase) among yeasts and their derivatives. Furthermore, supernatant from C. intermedia culture induced significant (p < 0.05) levels of TLR2 (4.4-fold increase), being the only one among supernatants to present high levels of relative mRNA transcription of TLR2. Data found in this work arouse interest in further studies on interaction between non-Saccharomyces yeasts and immune system cells, mainly referring to immunomodulatory capacity.

The ecological role of killer yeasts in natural communities of yeasts

1987 ◽  
Vol 33 (9) ◽  
pp. 783-796 ◽  
Author(s):  
William T. Starmer ◽  
Philip F. Ganter ◽  
Virginia Aberdeen ◽  
Marc-Andre Lachance ◽  
Herman J. Phaff
Keyword(s):  
Yeast Species ◽  
Kluyveromyces Lactis ◽  
Killer Toxin ◽  
Toxin Production ◽  
Killer Activity ◽  
Killer Yeast ◽  
Killer Toxins ◽  
Naturally Occurring ◽  
Pichia Kluyveri

The killer phenomenon of yeasts was investigated in naturally occurring yeast communities. Yeast species from communities associated with the decaying stems and fruits of cactus and the slime fluxes of trees were studied for production of killer toxins and sensitivity to killer toxins produced by other yeasts. Yeasts found in decaying fruits showed the highest incidence of killing activity (30/112), while yeasts isolated from cactus necroses and tree fluxes showed lower activity (70/699 and 11/140, respectively). Cross-reaction studies indicated that few killer-sensitive interactions occur within the same habitat at a particular time and locality, but that killer-sensitive reactions occur more frequently among yeasts from different localities and habitats. The conditions that should be optimal for killer activity were found in fruits and young rots of Opuntia cladodes where the pH is low. The fruit habitat appears to favor the establishment of killer species. Killer toxin may affect the natural distribution of the killer yeast Pichia kluyveri and the sensitive yeast Cryptococcus cereanus. Their distributions indicate that the toxin produced by P. kluyveri limits the occurrence of Cr. cereanus in fruit and Opuntia pads. In general most communities have only one killer species. Sensitive strains are more widespread than killer strains and few species appear to be immune to all toxins. Genetic study of the killer yeast P. kluyveri indicates that the mode of inheritance of killer toxin production is nuclear and not cytoplasmic as is found in Saccharomyces cerevisiae and Kluyveromyces lactis.

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