Cellobiose phosphorylase (EC 2.4.1.20) of Cellulomonas: occurrence, induction, and its role in cellobiose metabolism

1983 ◽  
Vol 135 (4) ◽  
pp. 241-249 ◽  
Author(s):  
Karl. -L. Schimz ◽  
Birgitt Broll ◽  
Birgit John
Keyword(s):  
Cellobiose Phosphorylase ◽  
Cellobiose Metabolism

scholarly journals Dissecting cellobiose metabolic pathway and its application in biorefinery through consolidated bioprocessing in Myceliophthora thermophila

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Jingen Li ◽  
Shuying Gu ◽  
Zhen Zhao ◽  
Bingchen Chen ◽  
Qian Liu ◽  
...  
Keyword(s):  
Malic Acid ◽  
Plant Biomass ◽  
Consolidated Bioprocessing ◽  
Cellulase Activity ◽  
Industrial Applications ◽  
Efficient Production ◽  
Myceliophthora Thermophila ◽  
Final Strain ◽  
Cellobiose Metabolism ◽  
Cost Efficient

Abstract Background Lignocellulosic biomass has long been recognized as a potential sustainable source for industrial applications. The costs associated with conversion of plant biomass to fermentable sugar represent a significant barrier to the production of cost-competitive biochemicals. Consolidated bioprocessing (CBP) is considered a potential breakthrough for achieving cost-efficient production of biomass-based fuels and commodity chemicals. During the degradation of cellulose, cellobiose (major end-product of cellulase activity) is catabolized by hydrolytic and phosphorolytic pathways in cellulolytic organisms. However, the details of the two intracellular cellobiose metabolism pathways in cellulolytic fungi remain to be uncovered. Results Using the engineered malic acid production fungal strain JG207, we demonstrated that the hydrolytic pathway by β-glucosidase and the phosphorolytic pathway by phosphorylase are both used for intracellular cellobiose metabolism in Myceliophthora thermophila, and the yield of malic acid can benefit from the energy advantages of phosphorolytic cleavage. There were obvious differences in regulation of the two cellobiose catabolic pathways depending on whether M. thermophila JG207 was grown on cellobiose or Avicel. Disruption of Mtcpp in strain JG207 led to decreased production of malic acid under cellobiose conditions, while expression levels of all three intracellular β-glucosidase genes were significantly up-regulated to rescue the impairment of the phosphorolytic pathway under Avicel conditions. When the flux of the hydrolytic pathway was reduced, we found that β-glucosidase encoded by bgl1 was the dominant enzyme in the hydrolytic pathway and deletion of bgl1 resulted in significant enhancement of protein secretion but reduction of malate production. Combining comprehensive manipulation of both cellobiose utilization pathways and enhancement of cellobiose uptake by overexpression of a cellobiose transporter, the final strain JG412Δbgl2Δbgl3 produced up to 101.2 g/L and 77.4 g/L malic acid from cellobiose and Avicel, respectively, which corresponded to respective yields of 1.35 g/g and 1.03 g/g, representing significant improvement over the starting strain JG207. Conclusions This is the first report of detailed investigation of intracellular cellobiose catabolism in cellulolytic fungus M. thermophila. These results provide insights that can be applied to industrial fungi for production of biofuels and biochemicals from cellobiose and cellulose.

scholarly journals Cellobiose Metabolism in Aerobacter aerogenes

1972 ◽  
Vol 247 (11) ◽  
pp. 3420-3423
Author(s):  
Richard E. Palmer ◽  
Richard L. Anderson
Keyword(s):  
Aerobacter Aerogenes ◽  
Cellobiose Metabolism

Engineering of cellobiose phosphorylase for glycoside synthesis

2011 ◽  
Vol 156 (4) ◽  
pp. 253-260 ◽  
Author(s):  
Manu R.M. de Groeve ◽  
Tom Desmet ◽  
Wim Soetaert
Keyword(s):  
Glycoside Synthesis ◽  
Cellobiose Phosphorylase

Cellobiose metabolism and cellobiohydrolase I biosynthesis by Trichoderma reesei

1990 ◽  
Vol 14 (4) ◽  
pp. 405-415 ◽  
Author(s):  
C. Fritscher ◽  
R. Messner ◽  
C.P. Kubicek
Keyword(s):  
Trichoderma Reesei ◽  
Cellobiohydrolase I ◽  
Cellobiose Metabolism

Relief of Xylose Binding to Cellobiose Phosphorylase by a Single Distal Mutation

2016 ◽  
Vol 6 (2) ◽  
pp. 206-210 ◽  
Author(s):  
Kulika Chomvong ◽  
Eric Lin ◽  
Michael Blaisse ◽  
Abigail E. Gillespie ◽  
Jamie H. D. Cate
Keyword(s):  
Cellobiose Phosphorylase ◽  
Distal Mutation

Energetic benefits and rapid cellobiose fermentation by Saccharomyces cerevisiae expressing cellobiose phosphorylase and mutant cellodextrin transporters

2013 ◽  
Vol 15 ◽  
pp. 134-143 ◽  
Author(s):  
Suk-Jin Ha ◽  
Jonathan M. Galazka ◽  
Eun Joong Oh ◽  
Vesna Kordić ◽  
Heejin Kim ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cellobiose Phosphorylase

Efficient chemoenzymatic oligosaccharide synthesis by reverse phosphorolysis using cellobiose phosphorylase and cellodextrin phosphorylase from Clostridium thermocellum

Biochimie ◽  
2010 ◽  
Vol 92 (12) ◽  
pp. 1818-1826 ◽  
Author(s):  
Hiroyuki Nakai ◽  
Maher Abou Hachem ◽  
Bent O. Petersen ◽  
Yvonne Westphal ◽  
Karin Mannerstedt ◽  
...  
Keyword(s):  
Clostridium Thermocellum ◽  
Oligosaccharide Synthesis ◽  
Cellobiose Phosphorylase

scholarly journals Cellobiose Phosphorylase in Fomes annosus

1973 ◽  
Vol 245 (141) ◽  
pp. 64-64 ◽  
Author(s):  
A. HÜTTERMANN ◽  
C. VOLGER
Keyword(s):  
Cellobiose Phosphorylase
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