Relief of Xylose Binding to Cellobiose Phosphorylase by a Single Distal Mutation

2016 ◽  
Vol 6 (2) ◽  
pp. 206-210 ◽  
Author(s):  
Kulika Chomvong ◽  
Eric Lin ◽  
Michael Blaisse ◽  
Abigail E. Gillespie ◽  
Jamie H. D. Cate
Keyword(s):  
Cellobiose Phosphorylase ◽  
Distal Mutation

Engineering of cellobiose phosphorylase for glycoside synthesis

2011 ◽  
Vol 156 (4) ◽  
pp. 253-260 ◽  
Author(s):  
Manu R.M. de Groeve ◽  
Tom Desmet ◽  
Wim Soetaert
Keyword(s):  
Glycoside Synthesis ◽  
Cellobiose Phosphorylase

scholarly journals Cooperative Binding of KaiB to the KaiC Hexamer Ensures Accurate Circadian Clock Oscillation in Cyanobacteria

2019 ◽  
Vol 20 (18) ◽  
pp. 4550 ◽  
Author(s):  
Reiko Murakami ◽  
Yasuhiro Yunoki ◽  
Kentaro Ishii ◽  
Kazuki Terauchi ◽  
Susumu Uchiyama ◽  
...  
Keyword(s):  
Mutational Analysis ◽  
Structural Data ◽  
Cooperative Interaction ◽  
Cooperative Binding ◽  
Rhythm Generation ◽  
Interaction Sites ◽  
Central Oscillator ◽  
Cooperative Assembly ◽  
Distal Mutation ◽  
Clock Protein

The central oscillator generating cyanobacterial circadian rhythms comprises KaiA, KaiB, and KaiC proteins. Their interactions cause KaiC phosphorylation and dephosphorylation cycles over approximately 24 h. KaiB interacts with phosphorylated KaiC in competition with SasA, an output protein harboring a KaiB-homologous domain. Structural data have identified KaiB–KaiC interaction sites; however, KaiB mutations distal from the binding surfaces can impair KaiB–KaiC interaction and the circadian rhythm. Reportedly, KaiB and KaiC exclusively form a complex in a 6:6 stoichiometry, indicating that KaiB–KaiC hexamer binding shows strong positive cooperativity. Here, mutational analysis was used to investigate the functional significance of this cooperative interaction. Results demonstrate that electrostatic complementarity between KaiB protomers promotes their cooperative assembly, which is indispensable for accurate rhythm generation. SasA does not exhibit such electrostatic complementarity and noncooperatively binds to KaiC. Thus, the findings explain KaiB distal mutation effects, providing mechanistic insights into clock protein interplay.

Energetic benefits and rapid cellobiose fermentation by Saccharomyces cerevisiae expressing cellobiose phosphorylase and mutant cellodextrin transporters

2013 ◽  
Vol 15 ◽  
pp. 134-143 ◽  
Author(s):  
Suk-Jin Ha ◽  
Jonathan M. Galazka ◽  
Eun Joong Oh ◽  
Vesna Kordić ◽  
Heejin Kim ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cellobiose Phosphorylase

Efficient chemoenzymatic oligosaccharide synthesis by reverse phosphorolysis using cellobiose phosphorylase and cellodextrin phosphorylase from Clostridium thermocellum

Biochimie ◽  
2010 ◽  
Vol 92 (12) ◽  
pp. 1818-1826 ◽  
Author(s):  
Hiroyuki Nakai ◽  
Maher Abou Hachem ◽  
Bent O. Petersen ◽  
Yvonne Westphal ◽  
Karin Mannerstedt ◽  
...  
Keyword(s):  
Clostridium Thermocellum ◽  
Oligosaccharide Synthesis ◽  
Cellobiose Phosphorylase

scholarly journals A Distal Mutation Perturbs Dynamic Amino Acid Networks in Dihydrofolate Reductase

Biochemistry ◽  
2013 ◽  
Vol 52 (27) ◽  
pp. 4605-4619 ◽  
Author(s):  
David D. Boehr ◽  
Jason R. Schnell ◽  
Dan McElheny ◽  
Sung-Hun Bae ◽  
Brendan M. Duggan ◽  
...  
Keyword(s):  
Amino Acid ◽  
Dihydrofolate Reductase ◽  
Distal Mutation

scholarly journals Cellobiose Phosphorylase in Fomes annosus

1973 ◽  
Vol 245 (141) ◽  
pp. 64-64 ◽  
Author(s):  
A. HÜTTERMANN ◽  
C. VOLGER
Keyword(s):  
Cellobiose Phosphorylase
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