Localization of mouse imprinted gene U2af1-rs1 to A3.2-4 band of Chromosome 11 by FISH

1994 ◽  
Vol 5 (10) ◽  
pp. 655-655 ◽  
Author(s):  
M. Tada ◽  
T. Tada ◽  
N. Takagi ◽  
Y. Hayashizaki ◽  
H. Shibata ◽  
...  
Keyword(s):  
Chromosome 11 ◽  
Imprinted Gene

scholarly journals Genomic Imprinting of Dopa decarboxylase in Heart and Reciprocal Allelic Expression with Neighboring Grb10

2007 ◽  
Vol 28 (1) ◽  
pp. 386-396 ◽  
Author(s):  
Trevelyan R. Menheniott ◽  
Kathryn Woodfine ◽  
Reiner Schulz ◽  
Andrew J. Wood ◽  
David Monk ◽  
...  
Keyword(s):  
Genomic Imprinting ◽  
Promoter Region ◽  
Germ Line ◽  
Differential Methylation ◽  
Dopa Decarboxylase ◽  
Imprinted Genes ◽  
Chromosome 11 ◽  
Imprinted Gene ◽  
Line Methylation ◽  
Intron 1

ABSTRACT By combining a tissue-specific microarray screen with mouse uniparental duplications, we have identified a novel imprinted gene, Dopa decarboxylase (Ddc), on chromosome 11. Ddc_exon1a is a 2-kb transcript variant that initiates from an alternative first exon in intron 1 of the canonical Ddc transcript and is paternally expressed in trabecular cardiomyocytes of the embryonic and neonatal heart. Ddc displays tight conserved linkage with the maternally expressed and methylated Grb10 gene, suggesting that these reciprocally imprinted genes may be coordinately regulated. In Dnmt3L mutant embryos that lack maternal germ line methylation imprints, we show that Ddc is overexpressed and Grb10 is silenced. Their imprinting is therefore dependent on maternal germ line methylation, but the mechanism at Ddc does not appear to involve differential methylation of the Ddc_exon1a promoter region and may instead be provided by the oocyte mark at Grb10. Our analysis of Ddc redefines the imprinted Grb10 domain on mouse proximal chromosome 11 and identifies Ddc_exon1a as the first example of a heart-specific imprinted gene.

scholarly journals Mouse U2af1-rs1 is a neomorphic imprinted gene.

1997 ◽  
Vol 17 (2) ◽  
pp. 789-798 ◽  
Author(s):  
A Nabetani ◽  
I Hatada ◽  
H Morisaki ◽  
M Oshimura ◽  
T Mukai
Keyword(s):  
Genomic Imprinting ◽  
Genome Structure ◽  
Proximal Region ◽  
Paternal Allele ◽  
Chromosome 2 ◽  
Chromosome 11 ◽  
Imprinted Gene ◽  
Genome Region ◽  
Allele Specific ◽  
Allele Specific Methylation

The mouse U2af1-rs1 gene is an endogenous imprinted gene on the proximal region of chromosome 11. This gene is transcribed exclusively from the unmethylated paternal allele, while the methylated maternal allele is silent. An analysis of genome structure of this gene revealed that the whole gene is located in an intron of the Murr1 gene. Although none of the three human U2af1-related genes have been mapped to chromosome 2, the human homolog of Murr1 is assigned to chromosome 2. The mouse Murr1 gene is transcribed biallelically, and therefore it is not imprinted in neonatal mice. Allele-specific methylation is limited to a region around U2af1-rs1 in an intron of Murr1. These results suggest that in chromosomal homology and genomic imprinting, the U2af1-rs1 gene is distinct from the genome region surrounding it. We have proposed the neomorphic origin of the U2af1-rs1 gene by retrotransposition and the particular mechanism of genomic imprinting of ectopic genes.

scholarly journals Identification of the Meg1/Grb10 imprinted gene on mouse proximal chromosome 11, a candidate for the Silver-Russell syndrome gene

1998 ◽  
Vol 95 (3) ◽  
pp. 1102-1107 ◽  
Author(s):  
N. Miyoshi ◽  
Y. Kuroiwa ◽  
T. Kohda ◽  
H. Shitara ◽  
H. Yonekawa ◽  
...  
Keyword(s):  
Chromosome 11 ◽  
Imprinted Gene ◽  
Silver Russell Syndrome

scholarly journals Imprinted Gene Expression and Function of the Dopa Decarboxylase Gene in the Developing Heart

2021 ◽  
Vol 9 ◽  
Author(s):  
Adam R. Prickett ◽  
Bertille Montibus ◽  
Nikolaos Barkas ◽  
Samuele M. Amante ◽  
Maurício M. Franco ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Knockout ◽  
Heart Defects ◽  
Abnormal Development ◽  
Dopa Decarboxylase ◽  
Mouse Heart ◽  
Chromosome 11 ◽  
Tissue Specific ◽  
Imprinted Gene ◽  
Developing Heart

Dopa decarboxylase (DDC) synthesizes serotonin in the developing mouse heart where it is encoded by Ddc_exon1a, a tissue-specific paternally expressed imprinted gene. Ddc_exon1a shares an imprinting control region (ICR) with the imprinted, maternally expressed (outside of the central nervous system) Grb10 gene on mouse chromosome 11, but little else is known about the tissue-specific imprinted expression of Ddc_exon1a. Fluorescent immunostaining localizes DDC to the developing myocardium in the pre-natal mouse heart, in a region susceptible to abnormal development and implicated in congenital heart defects in human. Ddc_exon1a and Grb10 are not co-expressed in heart nor in brain where Grb10 is also paternally expressed, despite sharing an ICR, indicating they are mechanistically linked by their shared ICR but not by Grb10 gene expression. Evidence from a Ddc_exon1a gene knockout mouse model suggests that it mediates the growth of the developing myocardium and a thinning of the myocardium is observed in a small number of mutant mice examined, with changes in gene expression detected by microarray analysis. Comparative studies in the human developing heart reveal a paternal expression bias with polymorphic imprinting patterns between individual human hearts at DDC_EXON1a, a finding consistent with other imprinted genes in human.

Linkage studies of NIDDM with 23 chromosome 11 markers in a sample of whites of northern European descent

Diabetes ◽  
1996 ◽  
Vol 45 (3) ◽  
pp. 370-375 ◽  
Author(s):  
S. C. Elbein ◽  
K. L. Bragg ◽  
M. D. Hoffman ◽  
R. A. Mayorga ◽  
M. F. Leppert
Keyword(s):  
Linkage Studies ◽  
Chromosome 11 ◽  
European Descent ◽  
Northern European
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