Dispersity of repeat DNA sequences in Oncopeltus fasciatus, an organism with diffuse centromeres

Chromosoma ◽  
1973 ◽  
Vol 43 (4) ◽  
pp. 349-373 ◽  
Author(s):  
J. M. Lagowski ◽  
Mei -Ying Wong Yu ◽  
H. S. Forrest ◽  
Charles D. Laird
Keyword(s):  
Dna Sequences ◽  
Oncopeltus Fasciatus ◽  
Repeat Dna

Differential distribution and association of repeat DNA sequences in the lateral element of the synaptonemal complex in rat spermatocytes

Chromosoma ◽  
2007 ◽  
Vol 117 (1) ◽  
pp. 77-87 ◽  
Author(s):  
Abrahan Hernández-Hernández ◽  
Héctor Rincón-Arano ◽  
Félix Recillas-Targa ◽  
Rosario Ortiz ◽  
Christian Valdes-Quezada ◽  
...  
Keyword(s):  
Synaptonemal Complex ◽  
Dna Sequences ◽  
Differential Distribution ◽  
Lateral Element ◽  
Repeat Dna

scholarly journals Totally Mutant Telomeres: Single-Step Mutagenesis of Tandem Repeat DNA Sequences

BioTechniques ◽  
2001 ◽  
Vol 30 (5) ◽  
pp. 934-938 ◽  
Author(s):  
Dana Hager Underwood ◽  
Michael J. McEachern
Keyword(s):  
Tandem Repeat ◽  
Dna Sequences ◽  
Single Step ◽  
Repeat Dna

scholarly journals Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences

2013 ◽  
Vol 24 (18) ◽  
pp. 2807-2819 ◽  
Author(s):  
Laura S. Burrack ◽  
Shelly E. Applen Clancey ◽  
Jeremy M. Chacón ◽  
Melissa K. Gardner ◽  
Judith Berman
Keyword(s):  
Chromosome Segregation ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Meiotic Chromosome ◽  
Higher Order ◽  
Order Structure ◽  
Cross Link ◽  
Microtubule Attachment ◽  
Sister Chromatids ◽  
Repeat Dna

The establishment and maintenance of higher-order structure at centromeres is essential for accurate chromosome segregation. The monopolin complex is thought to cross-link multiple kinetochore complexes to prevent merotelic attachments that result in chromosome missegregation. This model is based on structural analysis and the requirement that monopolin execute mitotic and meiotic chromosome segregation in Schizosaccharomyces pombe, which has more than one kinetochore–microtubule attachment/centromere, and co-orient sister chromatids in meiosis I in Saccharomyces cerevisiae. Recent data from S. pombe suggest an alternative possibility: that the recruitment of condensin is the primary function of monopolin. Here we test these models using the yeast Candida albicans. C. albicans cells lacking monopolin exhibit defects in chromosome segregation, increased distance between centromeres, and decreased stability of several types of repeat DNA. Of note, changing kinetochore–microtubule copy number from one to more than one kinetochore–microtubule/centromere does not alter the requirement for monopolin. Furthermore, monopolin recruits condensin to C. albicans centromeres, and overexpression of condensin suppresses chromosome segregation defects in strains lacking monopolin. We propose that the key function of monopolin is to recruit condensin in order to promote the assembly of higher-order structure at centromere and repetitive DNA.

scholarly journals Solution Formation of Holliday Junctions in Inverted-Repeat DNA Sequences†

Biochemistry ◽  
2006 ◽  
Vol 45 (8) ◽  
pp. 2467-2471 ◽  
Author(s):  
Franklin A. Hays ◽  
Virgil Schirf ◽  
P. Shing Ho ◽  
Borries Demeler
Keyword(s):  
Dna Sequences ◽  
Inverted Repeat ◽  
Holliday Junctions ◽  
Solution Formation ◽  
Repeat Dna

scholarly journals Atomic Force Microscopy and Voltammetric Investigation of Quadruplex Formation between a Triazole-Acridine Conjugate and Guanine-Containing Repeat DNA Sequences

2015 ◽  
Vol 87 (12) ◽  
pp. 6141-6149 ◽  
Author(s):  
Ana-Maria Chiorcea-Paquim ◽  
Ana Dora Rodrigues Pontinha ◽  
Ramon Eritja ◽  
Genny Lucarelli ◽  
Silvia Sparapani ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dna Sequences ◽  
Force Microscopy ◽  
Atomic Force ◽  
Repeat Dna ◽  
Quadruplex Formation

Detection of CAG repeat DNA sequences by pyrene-functionalized pyrrole-imidazole polyamides

2007 ◽  
Vol 15 (22) ◽  
pp. 6937-6942 ◽  
Author(s):  
Toshikazu Bando ◽  
Jun Fujimoto ◽  
Masafumi Minoshima ◽  
Ken-ichi Shinohara ◽  
Shunta Sasaki ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Cag Repeat ◽  
Repeat Dna

scholarly journals Parity-dependent hairpin configurations of repetitive DNA sequence promote slippage associated with DNA expansion

2017 ◽  
Vol 114 (36) ◽  
pp. 9535-9540 ◽  
Author(s):  
Tze-Yun Huang ◽  
Chung-ke Chang ◽  
Ya-Fen Kao ◽  
Chih-Hao Chin ◽  
Cheng-Wei Ni ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Single Molecule ◽  
Dna Sequences ◽  
Hot Spots ◽  
Neurological Diseases ◽  
Repeat Expansion ◽  
Single Molecule Spectroscopy ◽  
Dna Repeats ◽  
Structural Polymorphism ◽  
Repeat Dna

Repetitive DNA sequences are ubiquitous in life, and changes in the number of repeats often have various physiological and pathological implications. DNA repeats are capable of interchanging between different noncanonical and canonical conformations in a dynamic fashion, causing configurational slippage that often leads to repeat expansion associated with neurological diseases. In this report, we used single-molecule spectroscopy together with biophysical analyses to demonstrate the parity-dependent hairpin structural polymorphism of TGGAA repeat DNA. We found that the DNA adopted two configurations depending on the repeat number parity (even or odd). Transitions between these two configurations were also observed for longer repeats. In addition, the ability to modulate this transition was found to be enhanced by divalent ions. Based on the atomic structure, we propose a local seeding model where the kinked GGA motifs in the stem region of TGGAA repeat DNA act as hot spots to facilitate the transition between the two configurations, which may give rise to disease-associated repeat expansion.

scholarly journals The 2.1-kb Inverted Repeat DNA Sequences Flank themat2,3Silent Region in Two Species of Schizosaccharomyces and Are Involved in Epigenetic Silencing inSchizosaccharomyces pombe

Genetics ◽  
2002 ◽  
Vol 162 (2) ◽  
pp. 591-602 ◽  
Author(s):  
Gurjeet Singh ◽  
Amar J S Klar
Keyword(s):  
Dna Sequences ◽  
Inverted Repeat ◽  
Transcriptional Silencing ◽  
Epigenetic Silencing ◽  
Inverted Orientation ◽  
Repeat Dna ◽  
Sequence Elements ◽  
Left And Right ◽  
The Right ◽  
Silent Region

AbstractThe mat2,3 region of the fission yeast Schizosaccharomyces pombe exhibits a phenomenon of transcriptional silencing. This region is flanked by two identical DNA sequence elements, 2.1 kb in length, present in inverted orientation: IRL on the left and IRR on the right of the silent region. The repeats do not encode any ORF. The inverted repeat DNA region is also present in a newly identified related species, which we named S. kambucha. Interestingly, the left and right repeats share perfect identity within a species, but show ∼2% bases interspecies variation. Deletion of IRL results in variegated expression of markers inserted in the silent region, while deletion of the IRR causes their derepression. When deletions of these repeats were genetically combined with mutations in different trans-acting genes previously shown to cause a partial defect in silencing, only mutations in clr1 and clr3 showed additive defects in silencing with the deletion of IRL. The rate of mat1 switching is also affected by deletion of repeats. The IRL or IRR deletion did not cause significant derepression of the mat2 or mat3 loci. These results implicate repeats for maintaining full repression of the mat2,3 region, for efficient mat1 switching, and further support the notion that multiple pathways cooperate to silence the mat2,3 domain.

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