scholarly journals Solution Formation of Holliday Junctions in Inverted-Repeat DNA Sequences†

Biochemistry ◽  
2006 ◽  
Vol 45 (8) ◽  
pp. 2467-2471 ◽  
Author(s):  
Franklin A. Hays ◽  
Virgil Schirf ◽  
P. Shing Ho ◽  
Borries Demeler
Keyword(s):  
Dna Sequences ◽  
Inverted Repeat ◽  
Holliday Junctions ◽  
Solution Formation ◽  
Repeat Dna

scholarly journals The 2.1-kb Inverted Repeat DNA Sequences Flank themat2,3Silent Region in Two Species of Schizosaccharomyces and Are Involved in Epigenetic Silencing inSchizosaccharomyces pombe

Genetics ◽  
2002 ◽  
Vol 162 (2) ◽  
pp. 591-602 ◽  
Author(s):  
Gurjeet Singh ◽  
Amar J S Klar
Keyword(s):  
Dna Sequences ◽  
Inverted Repeat ◽  
Transcriptional Silencing ◽  
Epigenetic Silencing ◽  
Inverted Orientation ◽  
Repeat Dna ◽  
Sequence Elements ◽  
Left And Right ◽  
The Right ◽  
Silent Region

AbstractThe mat2,3 region of the fission yeast Schizosaccharomyces pombe exhibits a phenomenon of transcriptional silencing. This region is flanked by two identical DNA sequence elements, 2.1 kb in length, present in inverted orientation: IRL on the left and IRR on the right of the silent region. The repeats do not encode any ORF. The inverted repeat DNA region is also present in a newly identified related species, which we named S. kambucha. Interestingly, the left and right repeats share perfect identity within a species, but show ∼2% bases interspecies variation. Deletion of IRL results in variegated expression of markers inserted in the silent region, while deletion of the IRR causes their derepression. When deletions of these repeats were genetically combined with mutations in different trans-acting genes previously shown to cause a partial defect in silencing, only mutations in clr1 and clr3 showed additive defects in silencing with the deletion of IRL. The rate of mat1 switching is also affected by deletion of repeats. The IRL or IRR deletion did not cause significant derepression of the mat2 or mat3 loci. These results implicate repeats for maintaining full repression of the mat2,3 region, for efficient mat1 switching, and further support the notion that multiple pathways cooperate to silence the mat2,3 domain.

scholarly journals Cell-cycle-associated rearrangement of inverted repeat DNA sequences.

1979 ◽  
Vol 76 (12) ◽  
pp. 6240-6244 ◽  
Author(s):  
P. Nisen ◽  
R. Medford ◽  
J. Mansour ◽  
M. Purucker ◽  
A. Skalka ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Sequences ◽  
Inverted Repeat ◽  
Repeat Dna

scholarly journals An SHV-Derived Extended-Spectrum β-Lactamase in Pseudomonas aeruginosa

1999 ◽  
Vol 43 (5) ◽  
pp. 1281-1284 ◽  
Author(s):  
Thierry Naas ◽  
Laurence Philippon ◽  
Laurent Poirel ◽  
Esthel Ronco ◽  
Patrice Nordmann
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Dna Sequences ◽  
Inverted Repeat ◽  
Esbl Gene ◽  
Extended Spectrum ◽  
Its Gene

ABSTRACTA clinical isolate ofPseudomonas aeruginosaRP-1 produced the extended-spectrum β-lactamase (ESBL) SHV-2a. Its gene was expressed from a composite promoter made of the −35 region derived from the left inverted repeat of IS26and the −10 region from theblaSHV-2apromoter itself. The DNA sequences immediately surroundingblaSHV-2awere homologous to plasmid pMPA2a fromKlebsiella pneumoniaeKpZU-3, while further away and 3′ to theblaSHV-2agene, a sequence corresponding to the left end of Tn1721was detected, thus indicating a likely enterobacterial origin of this ESBL gene.

Differential distribution and association of repeat DNA sequences in the lateral element of the synaptonemal complex in rat spermatocytes

Chromosoma ◽  
2007 ◽  
Vol 117 (1) ◽  
pp. 77-87 ◽  
Author(s):  
Abrahan Hernández-Hernández ◽  
Héctor Rincón-Arano ◽  
Félix Recillas-Targa ◽  
Rosario Ortiz ◽  
Christian Valdes-Quezada ◽  
...  
Keyword(s):  
Synaptonemal Complex ◽  
Dna Sequences ◽  
Differential Distribution ◽  
Lateral Element ◽  
Repeat Dna

Characterization of two telomeric DNA processing reactions in Saccharomyces cerevisiae

1988 ◽  
Vol 8 (11) ◽  
pp. 4642-4650
Author(s):  
A W Murray ◽  
T E Claus ◽  
J W Szostak
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Division ◽  
Dna Polymerase ◽  
Dna Sequences ◽  
Inverted Repeat ◽  
Telomeric Sequence ◽  
Telomeric Dna ◽  
Telomeric Sequences ◽  
Telomere Elongation

We have investigated two reactions that occur on telomeric sequences introduced into Saccharomyces cerevisiae cells by transformation. The elongation reaction added repeats of the yeast telomeric sequence C1-3A to telomeric sequences at the end of linear DNA molecules. The reaction worked on the Tetrahymena telomeric sequence C4A2 and also on the simple repeat CA. The reaction was orientation specific: it occurred only when the GT-rich strand ran 5' to 3' towards the end of the molecule. Telomere elongation occurred by non-template-directed DNA synthesis rather than any type of recombination with chromosomal telomeres, because C1-3A repeats could be added to unrelated DNA sequences between the CA-rich repeats and the terminus of the transforming DNA. The elongation reaction was very efficient, and we believe that it was responsible for maintaining an average telomere length despite incomplete replication by template-directed DNA polymerase. The resolution reaction processed a head-to-head inverted repeat of telomeric sequences into two new telomeres at a frequency of 10(-2) per cell division.

scholarly journals Totally Mutant Telomeres: Single-Step Mutagenesis of Tandem Repeat DNA Sequences

BioTechniques ◽  
2001 ◽  
Vol 30 (5) ◽  
pp. 934-938 ◽  
Author(s):  
Dana Hager Underwood ◽  
Michael J. McEachern
Keyword(s):  
Tandem Repeat ◽  
Dna Sequences ◽  
Single Step ◽  
Repeat Dna

scholarly journals Characterization of two telomeric DNA processing reactions in Saccharomyces cerevisiae.

1988 ◽  
Vol 8 (11) ◽  
pp. 4642-4650 ◽  
Author(s):  
A W Murray ◽  
T E Claus ◽  
J W Szostak
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Division ◽  
Dna Polymerase ◽  
Dna Sequences ◽  
Inverted Repeat ◽  
Telomeric Sequence ◽  
Telomeric Dna ◽  
Telomeric Sequences ◽  
Telomere Elongation

We have investigated two reactions that occur on telomeric sequences introduced into Saccharomyces cerevisiae cells by transformation. The elongation reaction added repeats of the yeast telomeric sequence C1-3A to telomeric sequences at the end of linear DNA molecules. The reaction worked on the Tetrahymena telomeric sequence C4A2 and also on the simple repeat CA. The reaction was orientation specific: it occurred only when the GT-rich strand ran 5' to 3' towards the end of the molecule. Telomere elongation occurred by non-template-directed DNA synthesis rather than any type of recombination with chromosomal telomeres, because C1-3A repeats could be added to unrelated DNA sequences between the CA-rich repeats and the terminus of the transforming DNA. The elongation reaction was very efficient, and we believe that it was responsible for maintaining an average telomere length despite incomplete replication by template-directed DNA polymerase. The resolution reaction processed a head-to-head inverted repeat of telomeric sequences into two new telomeres at a frequency of 10(-2) per cell division.

Sign in / Sign up

Export Citation Format

Share Document