Diagnostic sensitivity of immunodominant epitopes of glutamic acid decarboxylase (GAD65) autoantibodies in childhood IDDM

Diabetologia ◽  
1996 ◽  
Vol 39 (9) ◽  
pp. 1091-1098 ◽  
Author(s):  
A. Falorni ◽  
M. Ackefors ◽  
C. Carlberg ◽  
T. Daniels ◽  
B. Persson ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Diagnostic Sensitivity ◽  
Acid Decarboxylase ◽  
Gad65 Autoantibodies ◽  
Childhood Iddm ◽  
Immunodominant Epitopes

Diagnostic sensitivity of immunodominant epitopes of glutamic acid decarboxylase (GAD65) autoantibodies in childhood IDDM

Diabetologia ◽  
1996 ◽  
Vol 39 (9) ◽  
pp. 1091-1098 ◽  
Author(s):  
A. Falorni ◽  
M. Ackefors ◽  
C. Carlberg ◽  
T. Daniels ◽  
B. Persson ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Diagnostic Sensitivity ◽  
Acid Decarboxylase ◽  
Gad65 Autoantibodies ◽  
Childhood Iddm ◽  
Immunodominant Epitopes

scholarly journals Characterization of the humoral immune response to glutamic acid decarboxylase in patients with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) and/or type 1 diabetes

2005 ◽  
Vol 153 (6) ◽  
pp. 901-906 ◽  
Author(s):  
Matti S Ronkainen ◽  
Taina Härkönen ◽  
Jaakko Perheentupa ◽  
Mikael Knip
Keyword(s):  
Immune Response ◽  
Type 1 Diabetes ◽  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Humoral Immune Response ◽  
Acid Decarboxylase ◽  
Humoral Immune ◽  
Gad65 Autoantibodies ◽  
Autoimmune Polyendocrinopathy

Objective: A humoral autoimmune response to glutamic acid decarboxylase (GAD65) is common both in patients with type 1 diabetes and in those with the autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) syndrome, while overt type 1 diabetes is relatively rarely diagnosed in APECED patients. The aim of this study was to assess whether this difference in the incidence of type 1 diabetes is associated with variability in the humoral immune response to GAD65, one of the major autoantigens in type 1 diabetes. Methods: Epitope- and isotype-specific GAD65 autoantibodies were analysed in 20 patients with APECED and 20 patients with newly diagnosed type 1 diabetes alone by radiobinding assays. Results: GAD65 autoantibodies targeted the middle and carboxy-terminal regions of GAD65 and occasionally the amino-terminal region in the APECED patients and comprised mainly the IgG1 subclass and less frequently the IgG2 and IgG4 subclasses. The profile of epitope- and isotype-specific GAD65 autoantibodies was similar in type 1 diabetes and APECED, except that IgG2 subclass antibodies were observed more often and at higher levels in the patients with type 1 diabetes alone (P < 0.05). None of the measured parameters separated APECED patients with type 1 diabetes from those without type 1 diabetes. Conclusion: APECED-associated humoral autoimmunity to GAD65 does not differ markedly from that observed in type 1 diabetes; only IgG2-GAD65 antibodies may be more closely associated with the latter entity.

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