Compartmentation of labeled fixation products in intact mesophyll protoplasts from Avena sativa L. after in-situ inhibition of the chloroplast phosphate translocator

Planta ◽  
1983 ◽  
Vol 159 (4) ◽  
pp. 314-321 ◽  
Author(s):  
R. Hampp ◽  
M. Goller
Keyword(s):  
Avena Sativa ◽  
Mesophyll Protoplasts ◽  
Phosphate Translocator

The genomic identification of some monosomics of Avena sativa L. cv. Sun II using genomic in situ hybridization

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 747-751 ◽  
Author(s):  
J. M. Leggett ◽  
G. S. Markhand
Keyword(s):  
In Situ Hybridization ◽  
Avena Sativa ◽  
Genomic Dna ◽  
Diploid Species ◽  
Genomic In Situ Hybridization ◽  
Chromosome Translocations ◽  
C Genome

Genomic in situ hybridization using total genomic DNA extracted from the C genome diploid species Avena eriantha (2n = 2x = 14, genome CpCp) was used to identify monosomics (2n = 6x − 1 = 41) of the constituent genomes of the hexaploid cultivated oat A. sativa L. cv. Sun II (2n = 6x = 42, genomes AACCDD). The results demonstrate 3 AD/C and 6 C/AD chromosome translocations, indicate that five of the missing monosomics are derived from the C genome, and show that there are duplicates within the partial monosomic series. Chromosome polymorphisms between some monosomic lines are also demonstrated.Key words: Avena, monosomics, genomic in situ hybridization, genomic identification.

scholarly journals Degradabilidad y estimación del consumo de forrajes y concentrados en alpacas (Vicugna pacos)

2018 ◽  
Vol 29 (2) ◽  
pp. 429
Author(s):  
Alfonso Cordero F. ◽  
José Contreras P. ◽  
James Curasma C. ◽  
Miguel Tunque Q. ◽  
Daniel Enríquez Q.
Keyword(s):  
Triticum Aestivum ◽  
Zea Mays ◽  
Solanum Tuberosum ◽  
Hordeum Vulgare ◽  
Avena Sativa ◽  
Zea Mays L ◽  
Triticum Aestivum L ◽  
Hordeum Vulgare L ◽  
Vicugna Pacos

El estudio tuvo como objetivo evaluar los parámetros cinéticos de la degradación in situ de la materia seca (MS), proteína cruda (PC) y la estimación del consumo mediante ecuaciones de predicción de MS de forrajes y alimentos concentrados en alpacas Huacaya (Vicugna pacos). Se trabajó con ensilado de maíz chala (Zea mays L) sin y con 1% de urea, cebada (Hordeum vulgare L), avena (Avena sativa L), salvado de trigo (Triticum aestivum L) y raspa de papa (Solanum tuberosum). Los alimentos (5 g en base seca) fueron colocados en sacos de nylon e incubados en el primer compartimento estomacal de dos alpacas fistuladas durante 0, 6, 12, 24, 48 y 76 horas. Se analizó la MS y la PC de los residuos de los sacos. La MS y la PC del salvado de trigo y de la raspa de papa presentaron potenciales de degradación elevados, así como la MS y la PC de la avena. Se destaca la mayor fracción no degradable de la PC del maíz chala sin y con urea y, por tanto, una menor degradabilidad de la PC. Las estimaciones del consumo por las alpacas generadas por las ecuaciones de tres estudios no son adecuadas a los alimentos en estudio.

Genomic in situ hybridization differentiates between A/D- and C-genome chromatin and detects intergenomic translocations in polyploid oat species (genus Avena)

Genome ◽  
1994 ◽  
Vol 37 (4) ◽  
pp. 613-618 ◽  
Author(s):  
E. N. Jellen ◽  
B. S. Gill ◽  
T. S. Cox
Keyword(s):  
In Situ Hybridization ◽  
Avena Sativa ◽  
Genomic In Situ Hybridization ◽  
Hybridization Pattern ◽  
C Banding ◽  
Intergenomic Translocations ◽  
C Genome ◽  
Total Dna

The genomic in situ hybridization (GISH) technique was used to discriminate between chromosomes of the C genome and those of the A and A/D genomes in allopolyploid oat species (genus Avena). Total biotinylated DNA from A. strigosa (2n = 2x = 14, AsAs genome) was mixed with sheared, unlabelled total DNA from A. eriantha (2n = 2x = 14, CpCp) at a ratio of 1:200 (labelled to unlabelled). The resulting hybridization pattern consisted of 28 mostly labelled and 14 mostly unlabelled chromosomes in the hexaploids. Attempts to discriminate between chromosomes of the A and D genomes in A. sativa (2n = 6x = 42, AACCDD) were unsuccessful using GISH. At least eight intergenomic translocation segments were detected in A. sativa 'Ogle', several of which were not observed in A. byzantina 'Kanota' (2n = 6x = 42, AACCDD) or in A. sterilis CW 439-2 (2n = 6x = 42, AACCDD). At least five intergenomic translocation segments were observed in A. maroccana CI 8330 'Magna' (2n = 4x = 28, AACC). In both 'Ogle' and 'Magna', positions of most of these translocations matched with C-banding patterns.Key words: Avena sativa, oat, in situ hybridization, C-banding, Avena macrostachya.

Exploring the origin of the D genome of oat by fluorescence in situ hybridization

Genome ◽  
2014 ◽  
Vol 57 (9) ◽  
pp. 469-472 ◽  
Author(s):  
Xiaomei Luo ◽  
Haiqin Zhang ◽  
Houyang Kang ◽  
Xing Fan ◽  
Yi Wang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Avena Sativa ◽  
Genetic Improvement ◽  
Similar Distribution ◽  
Diploid Progenitor ◽  
D Genome ◽  
Genome Donor ◽  
Sequential Experiments

Further understanding of the origin of cultivated oat would accelerate its genetic improvement. In particular, it would be useful to clarify which diploid progenitor contributed the D genome of this allohexaploid species. In this study, we demonstrate that the landmarks produced by fluorescence in situ hybridization (FISH) of species of Avena using probes derived from Avena sativa can be used to explore the origin of the D genome. Selected sets of probes were hybridized in several sequential experiments performed on exactly the same chromosome spreads, with multiple probes of cytological preparations. Probes pITS and A3-19 showed there might be a similar distribution of pITS between the Ac and D genomes. These results indicated that the Ac genome is closely related to the D genome, and that Avena canariensis (AcAc) could be the D-genome donor of cultivated oat.

Fluorescence in situ hybridization mapping of Avena sativa L. cv. SunII and its monosomic lines using cloned repetitive DNA sequences

Genome ◽  
2002 ◽  
Vol 45 (6) ◽  
pp. 1230-1237 ◽  
Author(s):  
M L Irigoyen ◽  
C Linares ◽  
E Ferrer ◽  
A Fominaya
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequences ◽  
Avena Sativa ◽  
Meiotic Chromosome ◽  
D Genome ◽  
Fish Mapping ◽  
Intergenomic Translocations ◽  
A Genome ◽  
C Genome

Fluorescent in situ hybridization (FISH) employing multiple probes was used with mitotic or meiotic chromosome spreads of Avena sativa L. cv. SunII and its monosomic lines to produce physical chromosome maps. The probes used were Avena strigosa pAs120a (which hybridizes exclusively to A-genome chromosomes), Avena murphyi pAm1 (which hybridizes exclusively to C-genome chromosomes), A. strigosa pAs121 (which hybridizes exclusively to A- and D-genome chromosomes), and the wheat rDNA probes pTa71 and pTa794. Simultaneous and sequential FISH employing two-by-two combinations of these probes allowed the unequivocal identification and genome assignation of all chromosomes. Ten pairs were found carrying intergenomic translocations: (i) between the A and C genomes (chromosome pair 5A); (ii) between the C and D genomes (pairs 1C, 2C, 4C, 10C, and 16C); and (iii) between the D and C genomes (pairs 9D, 11D, 13D, and 14D). The existence of a reciprocal intergenomic translocation (10C–14D) is also proposed. Comparing these results with those of other hexaploids, three intergenomic translocations (10C, 9D, and 14D) were found to be unique to A. sativa cv. SunII, supporting the view that 'SunII' is genetically distinct from other hexaploid Avena species and from cultivars of the A. sativa species. FISH mapping using meiotic and mitotic metaphases facilitated the genomic and chromosomal identification of the aneuploid chromosome in each monosomic line. Of the 18 analyzed, only 11 distinct monosomic lines were actually found, corresponding to 5 lines of the A genome, 2 lines of the C genome, and 4 lines of the D genome. The presence or absence of the 10C–14D interchange was also monitored in these lines.Key words: Avena sativa, monosomics, FISH mapping, genomic identification, intergenomic translocations.

Genomic in situ hybridization in Avena sativa

Genome ◽  
1994 ◽  
Vol 37 (4) ◽  
pp. 607-612 ◽  
Author(s):  
Qianfa Chen ◽  
Ken Armstrong
Keyword(s):  
In Situ Hybridization ◽  
Avena Sativa ◽  
Genome Organization ◽  
Fluorescent In Situ Hybridization ◽  
Fluorescein Isothiocyanate ◽  
Genomic Constitution ◽  
Avena Strigosa ◽  
Close Relationship ◽  
Genomic Dnas

Genomic fluorescent in situ hybridization was employed in the study of the genome organization and evolution of hexaploid oat (Avena sativa L. cv. Sun II, AACCDD, 2n = 6x = 42). Genomic DNAs from two diploid oat species, Avena strigosa (genomic constitution AsAs, 2n = 14) and Avena pilosa (genomic constitution CpCp, 2n = 14), were used as probes in the study. The DNA from A. strigosa labelled 28 of the 42 (2/3) chromosomes of the hexaploid oat, while 14 of the 42 (1/3) chromosomes were labelled with A. pilosa DNA, indicating a close relationship between the A and D genomes. Results also suggested that at least 18 chromosomes (9 pairs) were involved in intergenomic interchanges between the A and C genomes.Key words: oats, Avena sativa L., in situ hybridization, fluorescein isothiocyanate, genome organization.

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