The conversion of mevalonic acid into gibberellin A12-aldehyde in a cell-free system from Cucurbita pepo

Planta ◽  
1972 ◽  
Vol 102 (3) ◽  
pp. 261-271 ◽  
Author(s):  
J. E. Graebe ◽  
D. H. Bowen ◽  
J. MacMillan
Keyword(s):  
Cucurbita Pepo ◽  
Mevalonic Acid ◽  
Free System ◽  
Cell Free System ◽  
A Cell

A cell-free system from Streptococcus faecium for studies on the biosynthesis of triterpenoid carotenoids

1982 ◽  
Vol 60 (6) ◽  
pp. 675-683 ◽  
Author(s):  
Richard F. Taylor ◽  
Brian H. Davies
Keyword(s):  
Mevalonic Acid ◽  
Potassium Fluoride ◽  
Magnesium Ion ◽  
Free System ◽  
Isolation And Purification ◽  
Cell Free System ◽  
Manganese Ion ◽  
Absolute Requirement ◽  
Streptococcus Faecium ◽  
A Cell

A cell-free enzyme extract from Streptococcus faecium UNH 564P has been prepared. The extract incorporates either [2-14C]mevalonic acid (MVA) or [1-14C]isopentenyl pyrophosphate (IPP) into squalene and the carotenoids of the bacterium. ATP and manganese ion were found to be absolute requirements for MVA incorporation by the extract. Only manganese ion was found to be an absolute requirement for IPP incorporation by the extract. Other cofactors including magnesium ion, glutathione, potassium fluoride, NADP, and FAD significantly increase the incorporation of both substrates into the S. faecium terpenoids. Isolation and purification of the radioactive terpenoids from the cell-free system confirmed that the carotenoids of S. faecium are triterpenoids.

The biosynthesis of a C19-gibberellin from mevalonic acid in a cell-free system from a higher plant

Planta ◽  
1974 ◽  
Vol 120 (3) ◽  
pp. 307-309 ◽  
Author(s):  
J. E. Graebe ◽  
P. Hedden ◽  
P. Gaskin ◽  
J. MacMillan
Keyword(s):  
Mevalonic Acid ◽  
Higher Plant ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Effect of detergents on sterol synthesis in a cell-free system of yeast

1982 ◽  
Vol 23 (6) ◽  
pp. 803-810
Author(s):  
S Hata ◽  
T Nishino ◽  
N Ariga ◽  
H Katsuki
Keyword(s):  
Sterol Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Reconstitution of Endosomal Proteolysis in a Cell-free System

1989 ◽  
Vol 264 (10) ◽  
pp. 5392-5399
Author(s):  
L S Mayorga ◽  
R Diaz ◽  
P D Stahl
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell
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