The promotive effect of gibberellic acid on the production of adventitious roots on stem cuttings of Ipomoea fistulosa

Planta ◽  
1972 ◽  
Vol 105 (4) ◽  
pp. 360-363 ◽  
Author(s):  
K. K. Nanda ◽  
V. K. Anand ◽  
R. N. Chibbar
Keyword(s):  
Gibberellic Acid ◽  
Adventitious Roots ◽  
Stem Cuttings ◽  
Promotive Effect

Period of harvest, sprouting ability of cuttings, and in vitro plant regeneration in Fraxinus excelsior

1994 ◽  
Vol 72 (2) ◽  
pp. 261-267 ◽  
Author(s):  
Conceição Eneida Silveira ◽  
Alain Cottignies
Keyword(s):  
Chromosome Number ◽  
In Vitro Culture ◽  
Adventitious Roots ◽  
Woody Plant ◽  
Fraxinus Excelsior ◽  
Stem Cuttings ◽  
Natural Conditions ◽  
Apical Bud ◽  
Woody Plant Medium

Propagation by stem cuttings and in vitro culture of apical bud explants were studied on Fraxinus excelsior L. Stem cuttings from 4- to 7-year-old trees growing under natural conditions sprouted only when cuttings were taken from dormant material. Only 6% of those that had sprouted developed roots by the 7th month of culture. Similarly, only apical bud explants harvested during the dormant period sprouted in vitro. Up to 87% of these sprouts developed two to four branching adventitious roots after 5 months of culture. During the initial phase of in vitro culture, the Quoirin and Lepoivre medium and the woody plant medium favoured sprout lengthening. During the phase of multiplication, up to three sprouts per explant developed with the woody plant medium in the presence of a combination of high 6-benzylaminopurine (3.0–4.0 mg∙L−1) and low indole-3-butyric acid (0.01–0.03 mg∙L−1) concentrations. Rooting was obtained in a medium without any growth regulators. Microscopic analysis showed a direct connection between the vascular elements of adventitious roots and stem of plantlet. Chromosome number in root apices of ash plantlets and ash trees grown under natural conditions was 2n = 46. Key words: chromosome number, Fraxinus excelsior L., in vitro plants, micropropagation, stem cuttings.

scholarly journals Highly Successful Adventitious Root Formation of Zamia L. Stem Cuttings Exhibits Minimal Response to Indole-3-Butyric Acid

HortScience ◽  
2020 ◽  
Vol 55 (9) ◽  
pp. 1463-1467 ◽  
Author(s):  
Benjamin E. Deloso ◽  
Anders J. Lindström ◽  
Frank A. Camacho ◽  
Thomas E. Marler
Keyword(s):  
Butyric Acid ◽  
Root Development ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
The Other ◽  
Control Group ◽  
Stem Cuttings ◽  
Adventitious Root Development

The influences of indole-3-butyric acid (IBA) concentrations of 0–30 mg·g−1 on the success and speed of adventitious root development of Zamia furfuracea L.f. and Zamia integrifolia L.f. stem cuttings were determined. Root formation success for both species was greater than 95%. The IBA concentrations did not influence the speed of root development for Z. furfuracea, but the Z. integrifolia cuttings that received IBA concentration of 3 mg·g−1 generated adventitious roots more slowly than the cuttings in the control group. The ending dry weights of the stems, leaves, and roots were not influenced by IBA concentration for either species. Our results indicated that adventitious root formation on stem cuttings of these two Zamia species is successful without horticultural application of IBA. Additional IBA studies are needed on the other 300+ cycad species, especially those that are in a threatened category.

scholarly journals Producing Black Pepper (Piper Nigrum L. cv. ‘Kuching’) Rootstock in a Deep-Water Culture Hydroponic System

2021 ◽  
Vol 11 (2) ◽  
pp. 88-97
Author(s):  
Jie Hung King ◽  
Babirye Khadijah ◽  
Kian Huat Ong
Keyword(s):  
Root Length ◽  
Adventitious Roots ◽  
Silicate Solution ◽  
Growth Conditions ◽  
Black Pepper ◽  
Nutrient Composition ◽  
Piper Nigrum ◽  
Pepper Plant ◽  
Stem Cuttings ◽  
Hoagland Solution

Stem cutting is the common planting material for black pepper (Piper nigrum L.) farmers mainly because the method is cheap, easy to obtain, and produces satisfactory number of new plantlets, which are relatively genetically uniform to their parent. However, soil propagation of stem cuttings renders both the stem and developing roots susceptible to soil borne pathogens, ultimately compromising the quality of the plant. Good quality rootstock of the new plant promotes faster, safer, and better black pepper plant establishment. Hydroponic farming thus offers a good platform for producing quality rootstock of the new plants and has gained importance to many farmers due to its flexibility in manipulating plant growth conditions and timely pathogen management, thus safer, healthier, and faster growth. This study investigated the growing media suitable for rootstock growth of P. nigrum L. cv. ‘Kuching’ and compared the rooting ability between stem cuttings with adventitious roots at the time of planting and stem cuttings without any root at the time of planting. In a laboratory setting, a total of 210 stem cuttings were hydroponically planted in seven nutrient compositions, with each nutrient composition containing an equal number of stem cuttings with adventitious roots at the time of planting and stem cuttings without any root at the time of planting. Hoagland solution supplemented with 0.005 mM potassium silicate solution (T4) and Hoagland solution supplemented with 2 mM salicylic acid solution (T6) showed faster root initiation whereas T1 (Hoagland solution only) produced the highest increment in root length followed by T6. The least suitable nutrient composition was T5 [T4 + 6 mL of 1 M Ca (NO3)2.4H2O solution]. The total number of roots was highest in plants from stem cuttings which had some adventitious roots at the time of planting, whereas roots in plants from stem cuttings which did not have any root at the time of planting, increased in root length faster than plants withstem cuttings which had adventitious roots at the time of planting.

scholarly journals Establishment of an efficient micropropagation system in enhancing rooting efficiency via stem cuttings of apple rootstock M9T337

2021 ◽  
Author(s):  
Jiangli Shi ◽  
Zhidan Dong ◽  
Chunhui Song ◽  
Beiyang Xie ◽  
Xianbo Zheng ◽  
...  
Keyword(s):  
Adventitious Roots ◽  
Culture Media ◽  
Adventitious Shoots ◽  
Shoot Multiplication ◽  
Vital Role ◽  
Commercial Production ◽  
Stem Cuttings ◽  
Apple Rootstock ◽  
Environmental Adaptability

Rootstocks play a vital role in regulating the environmental adaptability and controlling the growth and development of apple trees. M9T337, an excellent apple rootstock widely used in commercial orchards, could confer dwarf tree architectures, early fruiting and suitability for high-density planting. However, the rooting ability of M9T3337 is low when it is vegetatively propagated, and researchers have not yet established an efficient micropropagation system. The present study systematically evaluated the multiplication in adventitious shoots and the in vitro formation of adventitious roots to determine the effects of the culture media and plant growth regulators of M9T337 and a rapid micropropagation system was developed. For the shoot multiplication, the highest multiplication index of 3.93 was obtained on Murashige and Skoog (MS) media supplemented with 2.0 mg/L 6-BA, 0.1 mg/L NAA and 0.3 mg/L GA3 from 12 combinations of 6-BA and NAA. Stronger and taller adventitious shoots were grown on MS supplemented with 1.8 mg/L 6-BA and 0.5 mg/L NAA. The optimal media with 100% rooting was obtained using ½ MS supplemented with 0.3 mg/L IBA or MS supplemented with 0.6 mg/L IBA for the rooting induction, resulting in mean rooting numbers of 13.00 and 11.33, respectively. Additionally, the effect on rooting of adding 0.3 mg/L IBA or not on the 1/2 MS and MS media was compared; the results suggested that an appropriate IBA concentration was the key to successful rooting. The rooted plantlets were acclimatised in a shaded greenhouse with an 84% survival rate. The established micropropagation system could be used for the rapid propagation of M9T337 for commercial production.

scholarly journals Anatomy of Adventitious Root Formation in Stem Cuttings of Mussaenda erythrophylla L. `Rosea'

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 634b-634
Author(s):  
Rolston St. Hilaire ◽  
Carlos A. Fierro Berwart
Keyword(s):  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Root Primordia ◽  
Fast Green ◽  
Phloem Parenchyma ◽  
Ornamental Shrubs ◽  
Vascular Connections

Mussaendas (Mussaenda spp.) are ornamental shrubs, and some cultivars are difficult to root. This study was conducted to explore how adventitious roots initiate and develop in the cultivar Rosea, and to determine if anatomical events are associated with difficulty in rooting stem cuttings. Stem cuttings were treated with 5, 10, 15 mm 1H-indole-3-butyric acid (IBA), or distilled water, and sampled every 2 days over 26 days to observe adventitious root formation and development. For analysis by light microscopy, the basal 1 cm of cuttings was embedded in wax and stained with safranin-fast green. Adventitious roots initiated from phloem parenchyma cells and from basal callus in nontreated cuttings. Cuttings treated with 15 mm IBA had a mean of 18 root primordia per basal 1 cm of cutting after 10 days. Root primordia were not observed in non-treated cuttings at 10 days. Root primordia that developed in non-treated cuttings lacked clear vascular connections. These results suggest that non-treated cuttings are difficult to root because few primordia are produced.

scholarly journals Change in Tissue Mineral Elemental Concentration during Root Initiation and Development of Poinsettia Cuttings

HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 617-619 ◽  
Author(s):  
Sven E. Svenson ◽  
Fred T. Davies
Keyword(s):  
Adventitious Roots ◽  
Elemental Concentration ◽  
Euphorbia Pulcherrima ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Root Primordia ◽  
Foliar N ◽  
Element Concentrations ◽  
Propagation Medium

Variation in tissue elemental concentration in apical stem cuttings of `Lilo' and `V-10 Amy' poinsettia (Euphorbia pulcherrima Willd. ex. Klotzch) were studied during the initiation and development of adventitious roots. Changes in selected macro- and micro-element concentrations coincided with root initiation (i.e., Fe, Cu, and Mo accumulated in the basal portions of stem cuttings during early root initiation before root primordia elongation); P, K, Ca, and Mg concentrations declined. During root primordia elongation and root emergence, Fe, Cu, and Mo and Mg, Mn, B, and Zn concentrations continued to increase at the cutting bases, but P and K concentrations remained low compared to when cuttings were initially inserted in the propagation medium. When all cutting of both cultivars had rooted, foliar N, Fe, and Mo concentrations declined, but Cu increased compared to when cuttings were initially propagated.

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