Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts

Planta ◽  
1970 ◽  
Vol 92 (4) ◽  
pp. 301-308 ◽  
Author(s):  
Toshiyuki Nagata ◽  
Itaru Takebe
Keyword(s):  
Cell Wall ◽  
Cell Division ◽  
Cell Wall Regeneration ◽  
Mesophyll Protoplasts ◽  
Wall Regeneration

scholarly journals Quantitative confocal imaging method for analyzing cellulose dynamics during cell wall regeneration in Arabidopsis mesophyll protoplasts

Plant Direct ◽  
2017 ◽  
Vol 1 (6) ◽  
pp. e00021 ◽  
Author(s):  
Hiroaki Kuki ◽  
Takumi Higaki ◽  
Ryusuke Yokoyama ◽  
Takeshi Kuroha ◽  
Naoki Shinohara ◽  
...  
Keyword(s):  
Cell Wall ◽  
Confocal Imaging ◽  
Imaging Method ◽  
Cell Wall Regeneration ◽  
Mesophyll Protoplasts ◽  
Wall Regeneration

Protoplast isolation and fusion in three Ulmus species

1981 ◽  
Vol 59 (8) ◽  
pp. 1436-1443 ◽  
Author(s):  
Keith Redenbaugh ◽  
David F. Karnosky ◽  
Robert D. Westfall
Keyword(s):  
Cell Wall ◽  
Cell Division ◽  
Protoplast Isolation ◽  
Suspension Cultures ◽  
Cell Wall Regeneration ◽  
Intact Cells ◽  
Ulmus Americana ◽  
Wall Regeneration

Protoplasts were isolated from cotyledons, callus, and suspension cultures of Ulmus americana L. and U. pumula L. and from cotyledons and callus of U. parvifolia Jacq. using various enzyme solutions. Isolation frequencies (percent protoplasts in a solution containing both protoplasts and broken or damaged protoplasts, but very few intact cells) were usually about 10%; however, for U. pumila cotyledons, protoplast frequencies reached 100%. In a limited number of cases, cell wall regeneration occurred after 4–21 days and cell division after 9–21 days. Finally, U. pumila protoplasts were fused using a PEG–calcium solution.

Factors that Influence the Yield, Stability in Culture and Cell Wall Regeneration of Spinach Mesophyll Protoplasts

1980 ◽  
Vol 7 (6) ◽  
pp. 713 ◽  
Author(s):  
R.J Rose
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Yield Stability ◽  
Developmental Studies ◽  
Cell Wall Regeneration ◽  
Mesophyll Cells ◽  
Isolation Medium ◽  
Mesophyll Protoplasts ◽  
Wall Regeneration ◽  
Ability To Regenerate

A study has been made of factors that influence the yield, stability in culture, and ability to regenerate cell walls of isolated spinach (Spinacia olevacea L.) mesophyll protoplasts. The presence of 7 mM CaZ + or 7 mM Mg2+ in the isolation medium, which also included 1.5 % (w/v) Driselase, 0.25 % (w/v) pectinase and 0.8 M sorbitol, increased the yield and stability in culture of the protoplasts in a liquid nutrient medium. This latter medium has been used previously in the culture of spinach leaf discs, and does not support the division of spinach mesophyll cells. Protoplasts isolated in the presence of CaZ+ showed a greater capacity for cell wall regeneration compared with protoplasts isolated in the absence of ions or in the presence of Mg2+. Though darkness during culture improved the initial protoplast stability, it inhibited wall regeneration. The initial stability of protoplasts appeared to be dependent on plasma membrane stability, but after a few days in culture the most effective treatments were those which stimulated cell wall regeneration. In many cases, associated with cell wall regeneration, there was a budding off of small vesicles which sometimes contained chloroplasts. Protoplasts isolated in the presence of Ca2+ and incubated in low light had a 50% survival rate after 8 days culture and most had regenerated cell walls. Such culture of spinach protoplasts has not been shown previously and should be useful in developmental studies of spinach chloroplasts.

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