Studies of cotyledon protoplast cultures from Brassica napus, B. campestris and B. oleracea. I: Cell wall regeneration and cell division

1995 ◽  
Vol 40 (1) ◽  
pp. 59-72 ◽  
Author(s):  
Kong-Nan Zhao ◽  
Dennis J. Bittisnich ◽  
Gerald M. Halloran ◽  
Malcolm I. Whitecross
Keyword(s):  
Cell Wall ◽  
Cell Division ◽  
Brassica Napus ◽  
Cell Wall Regeneration ◽  
Wall Regeneration

Protoplast isolation and fusion in three Ulmus species

1981 ◽  
Vol 59 (8) ◽  
pp. 1436-1443 ◽  
Author(s):  
Keith Redenbaugh ◽  
David F. Karnosky ◽  
Robert D. Westfall
Keyword(s):  
Cell Wall ◽  
Cell Division ◽  
Protoplast Isolation ◽  
Suspension Cultures ◽  
Cell Wall Regeneration ◽  
Intact Cells ◽  
Ulmus Americana ◽  
Wall Regeneration

Protoplasts were isolated from cotyledons, callus, and suspension cultures of Ulmus americana L. and U. pumula L. and from cotyledons and callus of U. parvifolia Jacq. using various enzyme solutions. Isolation frequencies (percent protoplasts in a solution containing both protoplasts and broken or damaged protoplasts, but very few intact cells) were usually about 10%; however, for U. pumila cotyledons, protoplast frequencies reached 100%. In a limited number of cases, cell wall regeneration occurred after 4–21 days and cell division after 9–21 days. Finally, U. pumila protoplasts were fused using a PEG–calcium solution.

scholarly journals Quantitative confocal imaging method for analyzing cellulose dynamics during cell wall regeneration in Arabidopsis mesophyll protoplasts

Plant Direct ◽  
2017 ◽  
Vol 1 (6) ◽  
pp. e00021 ◽  
Author(s):  
Hiroaki Kuki ◽  
Takumi Higaki ◽  
Ryusuke Yokoyama ◽  
Takeshi Kuroha ◽  
Naoki Shinohara ◽  
...  
Keyword(s):  
Cell Wall ◽  
Confocal Imaging ◽  
Imaging Method ◽  
Cell Wall Regeneration ◽  
Mesophyll Protoplasts ◽  
Wall Regeneration

Cell Wall Regeneration and Protoplast Reversion

2020 ◽  
pp. 115-133
Author(s):  
Oldřich Nečas ◽  
Augustin Svoboda
Keyword(s):  
Cell Wall ◽  
Cell Wall Regeneration ◽  
Wall Regeneration
Sign in / Sign up

Export Citation Format

Share Document