The movement of 2,4-dichlorophenoxy acetic acid in root segments of Pisum sativum L.

Planta ◽  
1975 ◽  
Vol 124 (2) ◽  
pp. 177-189 ◽  
Author(s):  
Henry Wilkins ◽  
Malcolm B. Wilkins
Keyword(s):  
Acetic Acid ◽  
Pisum Sativum ◽  
Root Segments ◽  
Pisum Sativum L ◽  
Dichlorophenoxy Acetic Acid

Callus formation from mesophyll protoplasts of Pisum sativum

1973 ◽  
Vol 51 (11) ◽  
pp. 2105-2106 ◽  
Author(s):  
F. Constabel ◽  
J. W. Kirkpatrick ◽  
O. L. Gamborg
Keyword(s):  
Acetic Acid ◽  
Pisum Sativum ◽  
Cell Walls ◽  
Callus Formation ◽  
Naphthaleneacetic Acid ◽  
Mesophyll Protoplasts ◽  
Leaf Material ◽  
Pisum Sativum L ◽  
Dichlorophenoxy Acetic Acid

Protoplasts isolated from mesophyll of Pisum sativum L. cv. Century, and cultured in 0.2-ml droplets of B5 medium with 1.0 mg/liter (2,4-dichlorophenoxy)acetic acid (2,4-D) or 1-naphthaleneacetic acid (NAA) and 2.0 mg/liter kinetin regenerated cell walls within 2–3 days. The resulting cells began to divide and form calli after 19 days of culture. Protoplast survival depended on keeping the leaf material in the dark for at least 30 h before use.

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