Control of synthesis de novo of ascorbate oxidase in the mustard seedling (Sinapis alba L.) by phytochrome

Planta ◽  
1974 ◽  
Vol 121 (1) ◽  
pp. 39-50 ◽  
Author(s):  
G. J. Acton ◽  
H. Drumm ◽  
H. Mohr
Keyword(s):  
De Novo ◽  
Sinapis Alba ◽  
Ascorbate Oxidase ◽  
Sinapis Alba L ◽  
Mustard Seedling

Experiments Regarding the Problem of Differentiation in Multicellular Systems

1970 ◽  
Vol 25 (11) ◽  
pp. 1282-1286 ◽  
Author(s):  
H. Oelze-Karow ◽  
H. Mohr
Keyword(s):  
Temporal Pattern ◽  
Control Mechanism ◽  
Sinapis Alba ◽  
Action Control ◽  
Multicellular Organism ◽  
Sinapis Alba L ◽  
Mustard Seedling

The temporal pattern of control by phytochrome of the enzyme lipoxygenase (EC 1.13.1.13) in the mustard seedling (Sinapis alba L.) is described. The biosynthetic system which produces lipoxygenase does not respond to phytochrome (Pfr) up to 33.25 h after sowing (25°C). By this time the control by Pfr comes suddenly and fully into play. At 48 h after sowing the system escapes from the control by Pfr. Neither the beginning nor the end of the period of control is influenced by phytochrome. Interpretation of the data requires a double action control mechanism. It is argued that such a mechanism is necessarily a characteristic of development in multicellular organism.

Control of Phenylalanine Ammonia-lyase and Ascorbate Oxidase in the Mustard Seedling by Light and Hoagland's Nutrient Solution

1974 ◽  
Vol 29 (5-6) ◽  
pp. 222-228 ◽  
Author(s):  
K. Peter ◽  
H. Mohr
Keyword(s):  
Nutrient Solution ◽  
Phenylalanine Ammonia Lyase ◽  
Red Light ◽  
Sinapis Alba ◽  
Ascorbate Oxidase ◽  
Activity Increase ◽  
Specific Effects ◽  
Mustard Seedling ◽  
Strong Stimulation ◽  
Stimulation Of

Phytochrome, Phenylalanine Ammonia-lyase, Ascorbate Oxidase, Hoagland's Solution, Mustard Seedling Hoagland's nutrient solution (HS) * was used as a tool to investigate whether or not the phyto­ chrome-mediated appearance of phenylalanine ammonia-lyase (PAL) * and ascorbate oxidase (AO) * in the mustard seedling cotyledons is simply a modulation of the appearance of these enzymes in the dark (basal level). HS was applied to the mustard (Sinapis alba L.) seedling at sowing instead of distilled water. It was observed that HS causes a stimulation of enzyme dis­ appearance (interpreted as degradation) in light and dark. This effect becomes obvious at approximately 48 h after sowing. On the other hand, however, HS exerts specific effects on the appearance of the enzymes in continuous far-red light (which is considered to operate exclusively via phytochrome). In the case of PAL there is a strong stimulation of the rate of enzyme appearance; however, the onset of activity increase remains precisely at 29 h after sowing irrespective of treatment. In the case of AO the onset of activity increase is shifted by approximately 6 h ; however, the rate of enzyme appearance remains the same (up to 54 h after sowing). The data suggest that HS specifically affects phytochrome-mediated enzyme syntheses whereas syntheses of the same enzymes in the dark are not affected. It is concluded that the appearance of PAL and AO in the dark and phytochrome-mediated appearance of these enzymes are independent phenomena. Some consequences of these conclusions for the interpretation of density labelling data obtained with "inducible" enzymes are discussed.

scholarly journals Phytotoxicity of Tires Evaluated in Simulated Conditions

Environments ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 49
Author(s):  
Markéta Šourková ◽  
Dana Adamcová ◽  
Jan Winkler ◽  
Magdalena Daria Vaverková
Keyword(s):  
Higher Plants ◽  
Sinapis Alba ◽  
Lepidium Sativum ◽  
Root Growth Inhibition ◽  
Chemical Parameters ◽  
Sinapis Alba L ◽  
Test Kit ◽  
Physical And Chemical ◽  
Aqueous Leachate ◽  
Lepidium Sativum L

Illegal dumps and landfills with disposed of tires are a fact of today, which should not be neglected as they represent a great ecological burden for the environment, affect the surrounding nature and disturb the landscape. This research was focused on testing the phytotoxicity of aqueous leachates from the fractions of tires in two sets of experiments—to simulate laboratory conditions (tire leaching in distilled water) and natural conditions (tire leaching in water from a recipient) using the Phytotoxkit testing kit (kit for the establishment of inhibition/stimulation effect on the root development) and the watercress test of phytotoxicity (biological method for the assessment of leachate phytotoxicity). Plants whose seeds were selected for the test were watercress (Lepidium sativum L.) and white mustard (Sinapis alba L.). The aqueous leachate was tested for 38 weeks. During the experiment, physical and chemical parameters were measured at intervals of 14 days by the testing instrument HACH TEST KIT: electric conductivity (EC), amount of dissolved oxygen (LDO) and pH. Results of root growth inhibition (IR) on the seeds of Lepidium sativum L. and Sinapis alba L. exhibited values ranging from 11.73% to 47.74% in the tested samples. Results of germination index (GI) on the seeds of Lepidium sativum L. exhibited values below 66% in the tested samples, which indicated the leachate phytotoxicity. In spite of the fact that similar studies are tackling the acute toxicity of leachates from tires (particularly to algae, embryos and animals), this research brings complementary information in testing the acute phytotoxicity of tire leachates to higher plants.

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