Fumigation-extraction and substrate-induced respiration derived microbial biomass C, and respiration rate in limed soil of Scots pine sapling stands

1994 ◽  
Vol 17 (4) ◽  
pp. 301-308 ◽  
Author(s):  
Outi Priha ◽  
Aino Smolander
Keyword(s):  
Microbial Biomass ◽  
Respiration Rate ◽  
Scots Pine ◽  
Microbial Biomass C ◽  
Substrate Induced Respiration ◽  
Limed Soil ◽  
Fumigation Extraction

Changes in carbon content, respiration rate, ATP content, and microbial biomass in nitrogen-fertilized pine forest soils in Sweden

1989 ◽  
Vol 19 (3) ◽  
pp. 323-328 ◽  
Author(s):  
H.-Ö. Nohrstedt ◽  
K. Arnebrant ◽  
E. Bååth ◽  
B. Söderström
Keyword(s):  
Microbial Biomass ◽  
Carbon Content ◽  
Respiration Rate ◽  
Microbial Biomass C ◽  
Absolute Amount ◽  
Fertilizer Treatment ◽  
Atp Content ◽  
Area Basis ◽  
Substrate Induced Respiration ◽  
And Control

Carbon content and indices of microbial biomass and activity were determined in 1985 in different soil horizons of two nitrogen-fertilized pine forests in Sweden. The Kroksbo site was fertilized in 1974 with 150 and 600 kg N•ha−1 using NH4NO3 or urea, while the Nissafors site was fertilized in 1977 and 1984 with 150 kg NH4NO3-N•ha−1 The absolute amount of C per square metre of forest floor increased in fertilizer treatments compared with the control (by 10–26%, depending on the site or fertilizer treatment). Respiration rate, ATP content, and microbial biomass C, measured with the substrate-induced respiration technique, decreased in all horizons when expressed per gram of C. The decrease was most evident with NH4NO3, and at the highest level of fertilization. However, on an area basis there were no differences between fertilizer and control treatments. A calculated increase in litter fall in the fertilized plots could not explain all the increase in the amount of C per square metre compared with the control. Decreased microbial activity per gram of C therefore appeared to be an important factor in the increase in C content in fertilized plots.

Soil C extracted with water or K2SO4: pH effect on determination of microbial biomass

1999 ◽  
Vol 79 (4) ◽  
pp. 529-533 ◽  
Author(s):  
R. L. Haney ◽  
A. J. Franzluebbers ◽  
F. M. Hons ◽  
D. A. Zuberer
Keyword(s):  
Microbial Biomass ◽  
Ph Effect ◽  
Calcareous Soils ◽  
Microbial Biomass C ◽  
Soil C ◽  
Soil Microbial ◽  
The Past ◽  
Chloroform Fumigation ◽  
Fumigation Extraction

Routine determination of soil microbial biomass C has shifted during the past decade from chloroform fumigation-incubation to chloroform fumigation-extraction using 0.5 M K2SO4 as extractant. We compared extractable C with water and 0.5 M K2SO4 in eight soils ranging in pH from 5.4 to 8.3. In unfumigated soils with low pH, extractable C was 0.8- to 1.2-fold greater with water than with 0.5 M K2SO4. However, in unfumigated soils with pH > 7.7, extractable C, although not statistically significant, was 11 to 19% less with water than with 0.5 M K2SO4. In fumigated soils, no difference in extractable C between water and 0.5 M K2SO4 was detected among soils with pH < 7.7, but extractable C was 13 to 17% less with water than with 0.5 M K2SO4 with pH > 7.7. Our results suggest that 0.5 M K2SO4 (1) may flocculate soil and cause adsorption of solubilized C onto colloids at pH < 7.7, but (2) may disperse calcareous soils at pH > 7.7, thereby differentially affecting the fate of solubilized C depending upon soil pH. Our results put into question the widespread adaptability of using chloroform fumigation-extraction to estimate microbial biomass C. Key words: Extractable carbon, chloroform fumigation-extraction, microbial biomass

Measurement of soil microbial biomass C by fumigation-extraction—an automated procedure

1990 ◽  
Vol 22 (8) ◽  
pp. 1167-1169 ◽  
Author(s):  
J. Wu ◽  
R.G. Joergensen ◽  
Birgit Pommerening ◽  
R. Chaussod ◽  
P.C. Brookes
Keyword(s):  
Microbial Biomass ◽  
Soil Microbial Biomass ◽  
Microbial Biomass C ◽  
Soil Microbial Biomass C ◽  
Soil Microbial ◽  
Fumigation Extraction

Quantifying the contribution from the soil microbail biomass to the extractable P levels of fresh and air-dried soils

Soil Research ◽  
1985 ◽  
Vol 23 (4) ◽  
pp. 613 ◽  
Author(s):  
GP Sparling ◽  
KN Whale ◽  
AJ Ramsay
Keyword(s):  
Microbial Biomass ◽  
Inorganic Phosphorus ◽  
Microbial Biomass C ◽  
Air Drying ◽  
P Values ◽  
Extractable P ◽  
P Content ◽  
Microbial P ◽  
Drying Treatment ◽  
Substrate Induced Respiration

Levels of P extractable in 0.5 M NaHCO3 and the microbial biomass C were measured on a range of 15 New Zealand pasture soils, with and without an air-drying treatment. The microbial biomass C, estimated using a modified substrate-induced-respiration method, was decreased 11-68% by air-drying. Two soils showed no increase in extractable inorganic phosphorus (P) levels after air-drying, but increases for the other 13 soils ranged over 14-184%. In general, the biomass C was not related to the overall P, levels of moist or air-dried soils. However, the increase in P, after air-drying was related to the microbial P content of the moist soil, estimated by CHCl3 fumigation, and the proportion of the biomass C killed by air-drying. A comparison of the actual measured Pi levels of dried soils and those estimated allowing for the microbial contribution, showed excellent agreement, strongly suggesting that the whole of the Pi increase on air-drying of soils was derived from killed microbial cells. The microbial contribution to Pi levels in NaHCO3 extracts of dried soils ranged over 4-76% and was therefore a significant large proportion in some soils. A large microbial contribution to Pi in extracts is most likely on air-dried soils from under pasture with >2% organic matter and NaHCO3-extractable P values of <20 �g g-1.

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