Light- and electron-microscopic immunochemical analysis of nerve fibre types innervating the taenia of the guinea-pig caecum

1992 ◽  
Vol 270 (1) ◽  
pp. 125-137 ◽  
Author(s):  
J. B. Furness ◽  
S. Pompolo ◽  
C. W. R. Shuttleworth ◽  
D. E. Burleigh
Keyword(s):  
Guinea Pig ◽  
Nerve Fibre ◽  
Fibre Types ◽  
Immunochemical Analysis ◽  
Electron Microscopic

scholarly journals STUDIES ON MICROPEROXISOMES II. A CYTOCHEMICAL METHOD FOR LIGHT AND ELECTRON MICROSCOPY

1972 ◽  
Vol 20 (12) ◽  
pp. 1006-1023 ◽  
Author(s):  
ALEX B. NOVIKOFF ◽  
PHYLLIS M. NOVIKOFF ◽  
CLEVELAND DAVIS ◽  
NELSON QUINTANA
Keyword(s):  
Endoplasmic Reticulum ◽  
Guinea Pig ◽  
Lipid Droplets ◽  
Smooth Endoplasmic Reticulum ◽  
Light And Electron Microscopy ◽  
Distal Tubule ◽  
Striking Difference ◽  
High Concentration ◽  
Electron Microscopic ◽  
Pig Kidney

A modification of the Novikoff-Goldfischer alkaline 3,3'-diaminobenzidine medium for visualizing peroxisomes is described. It makes possible light microscopic as well as electron microscopic studies of a recently described class of peroxisomes, the microperoxisomes. Potassium cyanide (5 x 10–3 M) is included in the medium to inhibit mitochondrial staining, the pH is 9.7 and there is a high concentration of H2O2 (0.05%). Two cell types have been chosen to illustrate the advantages of the new procedure for demonstrating the microperoxisomes: the absorptive cells in the human jejunum and the distal tubule cells in the guinea pig kidney. Suggestive relations of microperoxisomes and lipid are described in the human jejunum. The microperoxisomes are strategically located between smooth endoplasmic reticulum that radiates toward the organelles and contains lipid droplets and "central domains" of highly specialized endoplasmic reticulum which do not show the lipid droplets. The microperoxisomes are also present at the periphery of large lipid-like drops. In the guinea pig kidney tubule there is a striking difference between the thick limb of Henle and distal tubule. The distal tubule has a population of cells with large numbers of microperoxisomes readily visible by light microscopy; these cells are not present in the thick limb of Henle. Other differences between the two are also described.

scholarly journals PROBLEMS ASSOCIATED WITH THE DEMONSTRATION BY LEAD METHODS OF ADENOSINE TRIPHOSPHATASE ACTIVITY IN RESIDENT PERITONEAL MACROPHAGES AND EXUDATE MONOCYTES OF THE GUINEA PIG

1973 ◽  
Vol 21 (5) ◽  
pp. 488-498 ◽  
Author(s):  
R. E. POELMANN ◽  
W. T. DAEMS ◽  
E. J. VAN LOHUIZEN
Keyword(s):  
Plasma Membrane ◽  
Guinea Pig ◽  
Microscopic Study ◽  
Heterogeneous Nucleation ◽  
Adenosine Triphosphatase ◽  
Peritoneal Macrophages ◽  
Electron Microscopic ◽  
Adenosine Triphosphatase Activity ◽  
Eosinophilic Granulocytes ◽  
Membrane Bound

This cytochemical and electron microscopic study on peritoneal macrophages of the guinea pig has raised doubts concerning the validity of lead methods for the demonstration of plasma membrane-bound adenosine triphosphatase activity. The problems encountered are inherent in the use of lead ions as a capture reagent. The nonenzymatically formed precipitates reflect sites of heterogeneous nucleation specific for certain kinds of cells, e.g., resident peritoneal macrophages, eosinophilic granulocytes and, to a lesser degree, exudate monocytes. This type of precipitation is also catalyzed on the surface of nonbiologic matrices such as latex particles. Enzymatic processes may well occur, but they cannot be distinguished from nonenzymatic processes.

Studies on the fibre types of the guinea pig masticatory muscles

1974 ◽  
Vol 47 (4) ◽  
pp. 1171-1184 ◽  
Author(s):  
Masuda Koji ◽  
Takahashi Shoichiro ◽  
Kuriyama Hirosi
Keyword(s):  
Guinea Pig ◽  
Masticatory Muscles ◽  
Fibre Types

Expanded teased nerve fibre pathological conditions in disease association

2018 ◽  
Vol 90 (2) ◽  
pp. 138-140 ◽  
Author(s):  
Min Xu ◽  
Marcus Pinto ◽  
Chenjing Sun ◽  
Janean K Engelstad ◽  
P James Dyck ◽  
...  
Keyword(s):  
Nerve Fibre ◽  
B Cell Lymphoma ◽  
Disease Association ◽  
Pathological Diagnosis ◽  
Fibre Types ◽  
Polyglucosan Body ◽  
Cellular Debris ◽  
Amyloid Polyneuropathy ◽  
Student’S T ◽  
Student’S T Test

ObjectiveTo describe an expanded teased nerve fibre classification in disease association.MethodsWe reviewed four newly proposed teased nerve fibre types (Types J–M): Type J, rope-like fibres; K, fibril-like clumps of osmium positivity; L, cellular debris along and within nerve fibres; M, circular axonal inclusions surrounded by thin myelin. Different clinical pathological entities were studied for these fibre types including chronic inflammatory demyelinating polyradiculoneuropathy (CIDP: N=20); amyloid polyneuropathy (N=20); intraneural B-cell lymphoma (N=20) or adult-onset polyglucosan body disease (APBD: N=6) in comparison with 112 disease controls. Student’s t-test was used to test significance of association between the identified fibre types and the specific clinical diagnosis.ResultsEach fibre type significantly associated (p<0.001) with particular disease categories: Type J, 60% of CIDP cases; Type K, 75% of amyloid cases; Type L, 75% of intraneural lymphoma cases; Type M, 100% of APBD cases. Rarely were these fibres found in the other disease control cases ≤3% of cases. In three cases, the teased fibre findings were so striking additional paraffin nerve preparations were made to make the pathological diagnosis when initial paraffin sections were non-diagnostic.ConclusionsTeased nerve fibre Types J–M associate with commonly seen pathological diagnosis and are helpful in the consideration of specific neuropathy diagnoses.

Electron microscopic studies on guinea pig rib cartilage

1973 ◽  
Vol 146 (1) ◽  
pp. 83-102 ◽  
Author(s):  
Johan Thyberg ◽  
Stefan Nilsson ◽  
Ulf Friberg
Keyword(s):  
Guinea Pig ◽  
Electron Microscopic ◽  
Rib Cartilage ◽  
Microscopic Studies
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