Intracellular ATP modulates desensitization of acetylcholine receptors controlling chloride current in Lymnaea neurons

1993 ◽  
Vol 424 (5-6) ◽  
pp. 385-390
Author(s):  
Nataliya A. Lozovaya ◽  
Catherine A. Vulfius ◽  
Victor I. Ilyin
Keyword(s):  
Acetylcholine Receptors ◽  
Chloride Current ◽  
Intracellular Atp ◽  
Lymnaea Neurons

scholarly journals Anesthetic Treatment Blocks Synaptogenesis But Not Neuronal Regeneration of Cultured Lymnaea Neurons

2003 ◽  
Vol 90 (4) ◽  
pp. 2232-2239 ◽  
Author(s):  
Alyson J. Woodall ◽  
Hiroaki Naruo ◽  
David J. Prince ◽  
Zhong Ping Feng ◽  
William Winlow ◽  
...  
Keyword(s):  
Nerve Regeneration ◽  
Acetylcholine Receptors ◽  
Direct Evidence ◽  
Molluscan Neurons ◽  
Synaptic Connections ◽  
Surgical Interventions ◽  
Cholinergic Synaptic Transmission ◽  
Lymnaea Neurons

Trauma and injury necessitate the use of various surgical interventions, yet such procedures themselves are invasive and often interrupt synaptic communications in the nervous system. Because anesthesia is required during surgery, it is important to determine whether long-term exposure of injured nervous tissue to anesthetics is detrimental to regeneration of neuronal processes and synaptic connections. In this study, using identified molluscan neurons, we provide direct evidence that the anesthetic propofol blocks cholinergic synaptic transmission between soma-soma paired Lymnaea neurons in a dose-dependent and reversible manner. These effects do not involve presynaptic secretory machinery, but rather postsynaptic acetylcholine receptors were affected by the anesthetic. Moreover, we discovered that long-term (18–24 h) anesthetic treatment of soma-soma paired neurons blocked synaptogenesis between these cells. However, after several hours of anesthetic washout, synapses developed between the neurons in a manner similar to that seen in vivo. Long-term anesthetic treatment of the identified neurons visceral dorsal 4 (VD4) and left pedal dorsal 1 (LPeD1) and the electrically coupled Pedal A cluster neurons (PeA) did not affect nerve regeneration in cell culture as the neurons continued to exhibit extensive neurite outgrowth. However, these sprouted neurons failed to develop chemical (VD4 and LPeD1) and electrical (PeA) synapses as observed in their control counterparts. After drug washout, appropriate synapses did reform between the cells, although this synaptogenesis required several days. Taken together, this study provides the first direct evidence that the clinically used anesthetic propofol does not affect nerve regeneration. However, the formation of both chemical and electrical synapses is severely compromised in the presence of this drug. This study emphasizes the importance of short-term anesthetic treatment, which may be critical for the restoration of synaptic connections between injured neurons.

Nicotinic acetylcholine receptors in the patients with Parkinson's disease: 5IA-SPECT

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S586-S586 ◽  
Author(s):  
Kazuo Hashikawa ◽  
Hidefumi Yoshida ◽  
Nobukatsu Sawamoto ◽  
Shigetoshi Takaya ◽  
Chihiro Namiki ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Nicotinic Acetylcholine Receptors ◽  
Acetylcholine Receptors ◽  
Nicotinic Acetylcholine

Decreased insulin binding and degradation associated with depressed intracellular ATP content

Diabetes ◽  
1980 ◽  
Vol 29 (3) ◽  
pp. 221-226 ◽  
Author(s):  
B. Draznin ◽  
C. C. Solomons ◽  
C. A. Emler ◽  
D. S. Schalch ◽  
K. E. Sussman
Keyword(s):  
Insulin Binding ◽  
Atp Content ◽  
Intracellular Atp
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