scholarly journals Patterns of protein synthesis in the imaginal discs of Drosophila melanogaster: a comparison between different discs and stages

1993 ◽  
Vol 203 (3) ◽  
pp. 131-139 ◽  
Author(s):  
Juan F. Santar�n ◽  
Rosa Assiego ◽  
Antonio Garc�a-Bellido
Keyword(s):  
Protein Synthesis ◽  
Drosophila Melanogaster ◽  
Imaginal Discs

scholarly journals MAINTENANCE OF IMAGINAL DISCS OF DROSOPHILA MELANOGASTER IN CHEMICALLY DEFINED MEDIA

1969 ◽  
Vol 41 (3) ◽  
pp. 876-885 ◽  
Author(s):  
James A. Robb
Keyword(s):  
Protein Synthesis ◽  
Drosophila Melanogaster ◽  
Synthetic Medium ◽  
Sodium Concentration ◽  
Imaginal Discs ◽  
Magnesium Concentration ◽  
Insoluble Protein ◽  
Total Rna ◽  
Phosphate Buffered Saline

A phosphate-buffered saline and a chemically defined synthetic medium for in vitro maintenance of imaginal discs of Drosophila melanogaster were developed. The composition of the chemically defined medium was varied in order to optimize the incorporation of tritiated uridine into RNA and tritiated amino acids into acid-insoluble protein. The optimal ranges obtained were: pH, 6.75–7.35; osmolarity, 285–345 milliosmoles/liter; sodium concentration, 40–60 mM/liter; potassium concentration, 40–60 mM/liter; magnesium concentration, 0.5–3.5 mM/liter; calcium concentration, 0.3–1.5 mM/liter; and inorganic phosphate concentration, 1.5–4.0 mM/liter. The phosphate-buffered saline is superior to a commonly used insect Ringer solution in maintaining total RNA and acid-insoluble protein synthesis in culture. The chemically defined synthetic medium permits linear total RNA and acid-insoluble protein synthesis for more than 48 hr, DNA synthesis for several hours, normal differentiation to occur after 74 hr in vitro, and trypsinization of imaginal discs into single cell suspensions without developmental damage.

Patterns of protein synthesis in imaginal discs of drosophila melanogaster

Cell ◽  
1977 ◽  
Vol 12 (4) ◽  
pp. 915-921 ◽  
Author(s):  
Michael E. Rodgers ◽  
Allen Shearn
Keyword(s):  
Protein Synthesis ◽  
Drosophila Melanogaster ◽  
Imaginal Discs

Aspects of RNA and protein synthesis in imaginal discs of Drosophila melanogaster

1968 ◽  
Vol 102 (1) ◽  
pp. 1-14 ◽  
Author(s):  
J. W. Fristrom ◽  
L. Brothers ◽  
V. Mancebo ◽  
D. Stewart
Keyword(s):  
Protein Synthesis ◽  
Drosophila Melanogaster ◽  
Imaginal Discs ◽  
Rna And Protein Synthesis

scholarly journals Identical 3′-terminal octanucleotide sequence in 18S ribosomal ribonucleic acid from different eukaryotes. A proposed role for this sequence in the recognition of terminator codons

1974 ◽  
Vol 141 (3) ◽  
pp. 609-615 ◽  
Author(s):  
John Shine ◽  
Lynn Dalgarno
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Synthesis ◽  
Drosophila Melanogaster ◽  
Ribosomal Rna ◽  
Ribonucleic Acid ◽  
18S Rrna ◽  
Base Sequence ◽  
Terminal Sequence ◽  
Ribosomal Ribonucleic Acid ◽  
Rrna Species

The 3′-terminal sequence of 18S ribosomal RNA from Drosophila melanogaster and Saccharomyces cerevisiae was determined by stepwise degradation from the 3′-terminus and labelling with [3H]isoniazid. The sequence G-A-U-C-A-U-U-AOH was found at the 3′-terminus of both 18S rRNA species. Less extensive data for 18S RNA from a number of other eukaryotes are consistent with the same 3′-terminal sequence, and an identical sequence has previously been reported for the 3′-end of rabbit reticulocyte 18S rRNA (Hunt, 1970). These results suggest that the base sequence in this region is strongly conserved and may be identical in all eukaryotes. As the 3′-terminal hexanucleotide is complementary to eukaryotic terminator codons we discuss the possibility that the 3′-end of 18S rRNA may have a direct base-pairing role in the termination of protein synthesis.

scholarly journals Imaginal discs regulate developmental timing in Drosophila melanogaster

2008 ◽  
Vol 321 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Bradley C. Stieper ◽  
Mania Kupershtok ◽  
Michael V. Driscoll ◽  
Alexander W. Shingleton
Keyword(s):  
Drosophila Melanogaster ◽  
Imaginal Discs ◽  
Developmental Timing

Myogenesis in primary cell cultures from drosophila melanogaster: Protein synthesis and actin heterogeneity during development

Cell ◽  
1978 ◽  
Vol 13 (4) ◽  
pp. 589-598 ◽  
Author(s):  
Robert V. Storti ◽  
Sharon J. Horovitch ◽  
Matthew P. Scott ◽  
Alexander Rich ◽  
Mary Lou Pardue
Keyword(s):  
Protein Synthesis ◽  
Drosophila Melanogaster ◽  
Cell Cultures ◽  
Primary Cell ◽  
Primary Cell Cultures
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