Polytene chromosome sof monogenic and amphogenic Chrysomya species (Calliphoridae, diptera): analysis of banding patterns and in situ hybridization with Drosophila sex determining gene sequences

Chromosoma ◽  
1994 ◽  
Vol 103 (1) ◽  
pp. 16-30 ◽  
Author(s):  
Susanne Puchalla
Keyword(s):  
In Situ Hybridization ◽  
Polytene Chromosome ◽  
Gene Sequences ◽  
Banding Patterns

In Situ Hybridization Techniques for Electron Microscopy Detection of Human Papillomavirus Gene Sequences

1997 ◽  
Vol 20 (4) ◽  
pp. 337-341 ◽  
Author(s):  
A. Picazo ◽  
F. Gómez-Aguado ◽  
M.T . Corcuera ◽  
R. Roldán ◽  
E. Muñoz ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Gene Sequences

Chromosomal sublocalization of human gene sequences by in situ hybridization

1988 ◽  
Vol 11 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Hsiao-chen Chang ◽  
Douglas J. Jolly ◽  
Eva Biro ◽  
Oliver W. Jones ◽  
Theodore Friedmann
Keyword(s):  
In Situ Hybridization ◽  
Human Gene ◽  
Gene Sequences

Fluorescence in situ hybridization of bovine Alu-like sequences to bovine and ovine chromosomes

Genome ◽  
1993 ◽  
Vol 36 (5) ◽  
pp. 984-986 ◽  
Author(s):  
E. Rajcan-Separovic ◽  
M. P. Sabour
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Sequences ◽  
Chromosome Identification ◽  
Banding Patterns ◽  
Alu Sequences ◽  
Diffuse Distribution

Fluorescence in situ hybridization procedures have been applied to study the distribution of Alu-like sequences on bovine and ovine chromosomes. Unlike in man and mouse, where the Alu sequences produced discrete R-like bands, a more diffuse distribution of Alu-like sequences was observed on both bovine and ovine chromosomes. Under the conditions used, banding patterns useful for chromosome identification were not detected.Key words: bovine Alu-like repetitive sequences, bovine and ovine chromosomes, fluorescence in situ hybridization.

Characterization of Hordeum chilense chromosomes by C-banding and in situ hybridization using highly repeated DNA probes

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 435-442 ◽  
Author(s):  
A. Cabrera ◽  
B. Friebe ◽  
J. Jiang ◽  
B. S. Gill
Keyword(s):  
In Situ Hybridization ◽  
Relative Length ◽  
Hordeum Chilense ◽  
Addition Lines ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Rdna Probe ◽  
26S Rdna ◽  
C Banding

C-banding patterns of Hordeum chilense and of Triticum aestivum 'Chinese Spring' – H. chilense disomic addition lines were analyzed and compared with in situ hybridization patterns using a biotin-labeled highly repetitive Triticum tauschii DNA sequence, pAs1, and a wheat 18S–26S rDNA probe. All seven H. chilense chromosomes pairs and the added H. chilense chromosomes present in the addition lines were identified by their characteristic C-banding pattern. Chromosome morphology and banding patterns were similar to those of the corresponding chromosomes present in the parent H. chilense accession. A C-banded karyotype of the added H. chilense chromosomes was constructed and chromosome lengths, arm ratios, and relative length, as compared with chromosome 3B, were determined. The probe pAs1 was found to hybridize to specific areas on telomeres and interstitial sites along the chromosomes, allowing the identification of all seven pairs of the H. chilense chromosomes. Comparison of the patterns of distribution of the hybridization sites of clone pAs1 in the T. tauschii and H. chilense chromosomes was carried out by in situ hybridization on somatic metaphase chromosomes of the HchHchDD amphiploid. In situ hybridization using the 18S–26S rDNA probe confirmed that the H. chilense chromosomes 5Hch and 6Hch were carrying nucleolus organizer regions. The results are discussed on the basis of phylogenetic relationships between D and Hch genomes.Key words: Hordeum, Triticum, C-banding, in situ hybridization, phylogeny.

Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence

Genome ◽  
1996 ◽  
Vol 39 (1) ◽  
pp. 93-104 ◽  
Author(s):  
C. Pedersen ◽  
S. K. Rasmussen ◽  
I. Linde-Laursen
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Chromosome Identification ◽  
Satellite Sequence ◽  
Banding Patterns ◽  
Phylogenetic Studies ◽  
Triticeae Species ◽  
Gaa Repeats

The satellite sequence studied was primarily composed of GAA repeats organized in long tracts of heterochromatic DNA. Fluorescent in situ hybridization (FISH) with the GAA satellite (GAA banding) to the chromosomes of barley, wheat, rye, and other Triticeae species produced banding patterns similar to those obtained by N-banding. The GAA-banding patterns of barley are described in detail and those of 12 other Triticeae species are described briefly. In situ hybridization with the GAA-satellite sequence permits identification of all the chromosomes of barley. It is a valuable alternative to other banding techniques, especially in connection with physical gene mapping by FISH. The application of the GAA-satellite sequence for the characterization of genomes in phylogenetic studies of genera containing the sequence is discussed. Key words : Hordeum vulgare, Triticeae, GAA-satellite sequence, chromosome identification, genome differentiation.

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