Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence

Genome ◽  
1996 ◽  
Vol 39 (1) ◽  
pp. 93-104 ◽  
Author(s):  
C. Pedersen ◽  
S. K. Rasmussen ◽  
I. Linde-Laursen
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Chromosome Identification ◽  
Satellite Sequence ◽  
Banding Patterns ◽  
Phylogenetic Studies ◽  
Triticeae Species ◽  
Gaa Repeats

The satellite sequence studied was primarily composed of GAA repeats organized in long tracts of heterochromatic DNA. Fluorescent in situ hybridization (FISH) with the GAA satellite (GAA banding) to the chromosomes of barley, wheat, rye, and other Triticeae species produced banding patterns similar to those obtained by N-banding. The GAA-banding patterns of barley are described in detail and those of 12 other Triticeae species are described briefly. In situ hybridization with the GAA-satellite sequence permits identification of all the chromosomes of barley. It is a valuable alternative to other banding techniques, especially in connection with physical gene mapping by FISH. The application of the GAA-satellite sequence for the characterization of genomes in phylogenetic studies of genera containing the sequence is discussed. Key words : Hordeum vulgare, Triticeae, GAA-satellite sequence, chromosome identification, genome differentiation.

Use of Fluorescent In Situ Hybridization for Marker Chromosome Identification in Congenital and Neoplastic Disorders

1991 ◽  
Vol 96 (2) ◽  
pp. 203-210 ◽  
Author(s):  
Chris R. Schad ◽  
William J. Kraker ◽  
Syed M. Jalal ◽  
Martin S. Tallman ◽  
Harold N. Londer ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Marker Chromosome ◽  
Chromosome Identification ◽  
Neoplastic Disorders

Identification of the entire chromosome complement of bread wheat by two-colour FISH

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 589-593 ◽  
Author(s):  
C. Pedersen ◽  
P. Langridge
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dna Sequences ◽  
Banding Pattern ◽  
Aegilops Tauschii ◽  
Chromosome Complement ◽  
Chromosome Identification ◽  
Satellite Sequence ◽  
Entire Chromosome

Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obtained with the pHvG38 probe containing the GAA-satellite sequence was identical to the N-banding pattern of wheat. A detailed idiogram was constructed, including 73 GAA bands and 48 pAs1 bands. Identification of the wheat chromosomes by FISH will be particularly useful in connection with the physical mapping of other DNA sequences to chromosomes, or for chromosome identification in general, as an alternative to C-banding.Key words: Triticum aestivum, chromosome identification, fluorescence in situ hybridization, repetitive DNA sequences.

Karyotypic characterization of the great sturgeon, Huso huso , by multiple staining techniques and fluorescent in situ hybridization

1998 ◽  
Vol 132 (3) ◽  
pp. 495-501 ◽  
Author(s):  
F. Fontana ◽  
J. Tagliavini ◽  
L. Congiu ◽  
M. Lanfredi ◽  
M. Chicca ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Huso Huso ◽  
Great Sturgeon ◽  
Staining Techniques

Fluorescent in situ hybridization with arbitrarily amplified DNA fragments differentiates carrot (Daucus carota L.) chromosomes

Genome ◽  
2012 ◽  
Vol 55 (3) ◽  
pp. 205-213 ◽  
Author(s):  
Anna Nowicka ◽  
Ewa Grzebelus ◽  
Dariusz Grzebelus
Keyword(s):  
In Situ Hybridization ◽  
Daucus Carota ◽  
Fluorescent In Situ Hybridization ◽  
Ltr Retrotransposons ◽  
Chromosome Identification ◽  
Daucus Carota L ◽  
Poorly Differentiated ◽  
End Sequences ◽  
Size And Morphology

Carrot ( Daucus carota L.) chromosomes are small and poorly differentiated in size and morphology. Here we demonstrate that fluorescent in situ hybridization (FISH) signals derived from arbitrary PCR probes can be used for chromosome identification in carrot. To prepare probes, we searched for nonpolymorphic products abundantly amplified with arbitrary decamer primers in a group of accessions representing carrot genetic diversity. As a result, 13 fragments ranging in size from 517 to 1758 bp were selected, sequenced, and used as probes for fluorescent in situ hybridization. Four of these probes produced clear and reproducible hybridization signals. The sequences showed similarity to a number of carrot BAC-end sequences, indicating their repetitive character. Three of them were similar to internal portions of gypsy and copia LTR retrotransposons previously identified in plants. Hybridization signals for the four probes were observed as dotted tracks on chromosomes, differing in distribution and intensity. Generally, they were present in pericentromeric and (or) interstitial localizations on chromosome arms. The use of the four probes allowed discrimination of chromosome pairs and construction of more detailed karyotypes and idiograms of carrot.

Isolation and characterization of circulating melanoma cells by size filtration and fluorescent in-situ hybridization

2018 ◽  
Vol 28 (2) ◽  
pp. 89-95 ◽  
Author(s):  
Masahiko Yanagita ◽  
Jason J. Luke ◽  
Frank S. Hodi ◽  
Pasi A. Jänne ◽  
Cloud P. Paweletz
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Melanoma Cells ◽  
Isolation And Characterization

Fluorescent in situ hybridization with rDNA probes on chromosomes of Acipenser ruthenus and Acipenser naccarii (Osteichthyes Acipenseriformes)

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 1008-1012 ◽  
Author(s):  
F Fontana ◽  
M Lanfredi ◽  
M Chicca ◽  
L Congiu ◽  
J Tagliavini ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
5S Rdna ◽  
28S Rdna ◽  
Detailed Characterization ◽  
Acipenser Ruthenus ◽  
Sturgeon Species ◽  
Sterlet Acipenser Ruthenus

The genes for 28S and 5S rDNA were physically mapped on the chromosomes of two sturgeon species, the sterlet (Acipenser ruthenus, 2n = 118 ± 4) and the Adriatic sturgeon (Acipenser naccarii, 2n = 248 ± 4) by fluorescent in situ hybridization. In the sterlet, the 28S rDNA was located on six chromosomes, four of which actively transcribed, while in the Adriatic sturgeon the 28S rDNA was located on a chromosome number ranging from 10 to 12, eight of which actively transcribed. The 5S rDNA was physically mapped on two chromosomes in the sterlet and on four in the Adriatic sturgeon. A more detailed characterization of the latter karyotype was obtained during this study. All these data are discussed in connection with the ploidy relationships among sturgeon species.Key words: karyotype, ploidy, FISH, 28S and 5S rDNA.

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