The absence of globin gene sequences from friend leukaemia virus RNA

1973 ◽  
Vol 1 (4) ◽  
pp. 221-224
Author(s):  
I. B. Pragnell ◽  
R. S. Gilmour
Keyword(s):  
Globin Gene ◽  
Gene Sequences ◽  
Leukaemia Virus ◽  
Virus Rna

Suggestive Evidence for an Oncorna-Virus-Specific DNA Polymerase from C-Type Particles of Bovine Leukosis

1974 ◽  
Vol 29 (1-2) ◽  
pp. 72-75 ◽  
Author(s):  
B. Dietzschold ◽  
O.R. Kaaden ◽  
S. Ueberschaer ◽  
F. Weiland ◽  
O. C. Straub
Keyword(s):  
Dna Polymerase ◽  
Polymerase Activity ◽  
Friend Virus ◽  
Leukaemia Virus ◽  
Virus Particles ◽  
Virus Rna ◽  
Feline Leukaemia Virus ◽  
Bovine Leukosis ◽  
Bovine Lymphocytes ◽  
Viral Preparation

Abstract Typical C-type oncorna virus particles as shown by electron microscopy have been purified from the supernatant of cultured lymphocytes from bovine leukosis. In the purified C-particle fraction a DNA-polymerase activity was detected. Using several synthetic RNA-or DNA-homopolymers and 70S Friend virus RNA the template response of this bovine leukosis cell particle DNA polymerase was compared with those of feline leukaemia virus DNA polymerase and DNA polymerase from normal bovine lymphocytes. The DNA polymerase detected in the viral preparation of bovine leukosis is suggested to be an oncorna-virus-specific enzyme.

scholarly journals The detection and use of hemoglobin mutants in the direct analysis of human globin genes

Blood ◽  
1980 ◽  
Vol 55 (6) ◽  
pp. 1060-1062 ◽  
Author(s):  
PF Little ◽  
E Whitelaw ◽  
G Annison ◽  
R Williamson ◽  
JM Kooter ◽  
...  
Keyword(s):  
Restriction Site ◽  
Globin Gene ◽  
Direct Analysis ◽  
Globin Genes ◽  
Beta Globin ◽  
Gene Sequences ◽  
Gamma Globin ◽  
Alpha Globin ◽  
Specific Restriction ◽  
Alpha Beta

Abstract Many human globin-chain mutants contain amino acid replacements that result from single base changes in the corresponding globin gene. Using recombinants, the coding sequences of each of the alpha-, beta-, Ggamma- , and Agamma-globin genes have now been determined. Those sequences of DNA that are cleaved by a number of specific restriction endonucleases have been identified and accurately positioned. Mutations at these sequences abolish the restriction site, and therefore, the pattern of DNA fragments containing hybridizing globin-gene sequences is altered compared to DNA from normal persons. This allows the identification of one of a pair of cross-hybridizing human globin-gene sequences, as is shown here for the two alpha-globin, the two gamma-globin, and the delta- and beta-globin genes.

Locus control region activity by 5′HS3 requires a functional interaction with β-globin gene regulatory elements: expression of novel β/γ-globin hybrid transgenes

Blood ◽  
2000 ◽  
Vol 95 (10) ◽  
pp. 3242-3249 ◽  
Author(s):  
Joel E. Rubin ◽  
Peter Pasceri ◽  
Xiumei Wu ◽  
Philippe Leboulch ◽  
James Ellis
Keyword(s):  
Gene Therapy ◽  
Transgenic Mice ◽  
Control Region ◽  
Globin Gene ◽  
Single Copy ◽  
Gene Sequences ◽  
Coding Sequences ◽  
Globin Promoter ◽  
Intron 2 ◽  
Chromatin Opening

Abstract The human β-globin locus control region (LCR) contains chromatin opening and transcriptional enhancement activities that are important to include in β-globin gene therapy vectors. We previously used single-copy transgenic mice to map chromatin opening activity to the 5′HS3 LCR element. Here, we test novel hybrid globin genes to identify β-globin gene sequences that functionally interact with 5′HS3. First, we show that an 850-base pair (bp) 5′HS3 element activates high-level β-globin gene expression in fetal livers of 17 of 17 transgenic mice, including 3 single-copy animals, but fails to reproducibly activate Aγ-globin transgenes. To identify the β-globin gene sequences required for LCR activity by 5′HS3, we linked the 815-bp β-globin promoter to Aγ-globin coding sequences (BGT34), together with either the β-globin intron 2 (BGT35), the β-globin 3′ enhancer (BGT54), or both intron 2 and the 3′ enhancer (BGT50). Of these transgenes, only BGT50 reproducibly expresses Aγ-globin RNA (including 7 of 7 single-copy animals, averaging 71% per copy). Modifications to BGT50 show that LCR activity is detected after replacing the β-globin promoter with the 700-bp Aγ-globin promoter, but is abrogated when an AT-rich region is deleted from β-globin intron 2. We conclude that LCR activity by 5′HS3 on globin promoters requires the simultaneous presence of β-globin intron 2 sequences and the 260-bp 3′ β-globin enhancer. The BGT50 construct extends the utility of the 5′HS3 element to include erythroid expression of nonadult β-globin coding sequences in transgenic animals and its ability to express antisickling γ-globin coding sequences at single copy are ideal characteristics for a gene therapy cassette.

Evidence on primate phylogeny from ?-globin gene sequences and flanking regions

1995 ◽  
Vol 40 (1) ◽  
pp. 30-55 ◽  
Author(s):  
Calvin A. Porter ◽  
Iracilda Sampaio ◽  
Horacio Schneider ◽  
Maria Paula C. Schneider ◽  
John Czelusniak ◽  
...  
Keyword(s):  
Globin Gene ◽  
Gene Sequences ◽  
Primate Phylogeny ◽  
Flanking Regions

Murine leukaemia virus RNA transcription from chromatin of normal and infected BALB/c spleen

Nature ◽  
1974 ◽  
Vol 251 (5473) ◽  
pp. 347-350 ◽  
Author(s):  
M. JANOWSKI ◽  
L. BAUGNET-MAHIEU ◽  
A. SASSEN
Keyword(s):  
Rna Transcription ◽  
Leukaemia Virus ◽  
Murine Leukaemia Virus ◽  
Virus Rna

scholarly journals The Subunits of Murine Sarcoma-Leukaemia Virus RNA

1973 ◽  
Vol 18 (1) ◽  
pp. 69-74 ◽  
Author(s):  
B. McCain ◽  
N. Biswal ◽  
M. Benyesh-Melnick
Keyword(s):  
Leukaemia Virus ◽  
Virus Rna ◽  
Murine Sarcoma

Molecular Phylogeny of Ateline New World Monkeys (Platyrrhini, Atelinae) Based on γ-Globin Gene Sequences: Evidence ThatBrachytelesIs the Sister Group ofLagothrix

1999 ◽  
Vol 12 (1) ◽  
pp. 10-30 ◽  
Author(s):  
Carla M. Meireles ◽  
John Czelusniak ◽  
Maria P.C. Schneider ◽  
Jose A.P.C. Muniz ◽  
Maria C. Brigido ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
New World ◽  
Globin Gene ◽  
Sister Group ◽  
New World Monkeys ◽  
Gene Sequences

Purification of large native DNA fragments enriched in globin gene sequences

Gene ◽  
1978 ◽  
Vol 3 (1) ◽  
pp. 81-85 ◽  
Author(s):  
A.P. Ryskov ◽  
N.E. Maleeva ◽  
S.A. Limborska
Keyword(s):  
Globin Gene ◽  
Dna Fragments ◽  
Gene Sequences
Sign in / Sign up

Export Citation Format

Share Document