Evidence on primate phylogeny from ?-globin gene sequences and flanking regions

1995 ◽  
Vol 40 (1) ◽  
pp. 30-55 ◽  
Author(s):  
Calvin A. Porter ◽  
Iracilda Sampaio ◽  
Horacio Schneider ◽  
Maria Paula C. Schneider ◽  
John Czelusniak ◽  
...  
Keyword(s):  
Globin Gene ◽  
Gene Sequences ◽  
Primate Phylogeny ◽  
Flanking Regions

The absence of globin gene sequences from friend leukaemia virus RNA

1973 ◽  
Vol 1 (4) ◽  
pp. 221-224
Author(s):  
I. B. Pragnell ◽  
R. S. Gilmour
Keyword(s):  
Globin Gene ◽  
Gene Sequences ◽  
Leukaemia Virus ◽  
Virus Rna

scholarly journals The detection and use of hemoglobin mutants in the direct analysis of human globin genes

Blood ◽  
1980 ◽  
Vol 55 (6) ◽  
pp. 1060-1062 ◽  
Author(s):  
PF Little ◽  
E Whitelaw ◽  
G Annison ◽  
R Williamson ◽  
JM Kooter ◽  
...  
Keyword(s):  
Restriction Site ◽  
Globin Gene ◽  
Direct Analysis ◽  
Globin Genes ◽  
Beta Globin ◽  
Gene Sequences ◽  
Gamma Globin ◽  
Alpha Globin ◽  
Specific Restriction ◽  
Alpha Beta

Abstract Many human globin-chain mutants contain amino acid replacements that result from single base changes in the corresponding globin gene. Using recombinants, the coding sequences of each of the alpha-, beta-, Ggamma- , and Agamma-globin genes have now been determined. Those sequences of DNA that are cleaved by a number of specific restriction endonucleases have been identified and accurately positioned. Mutations at these sequences abolish the restriction site, and therefore, the pattern of DNA fragments containing hybridizing globin-gene sequences is altered compared to DNA from normal persons. This allows the identification of one of a pair of cross-hybridizing human globin-gene sequences, as is shown here for the two alpha-globin, the two gamma-globin, and the delta- and beta-globin genes.

Locus control region activity by 5′HS3 requires a functional interaction with β-globin gene regulatory elements: expression of novel β/γ-globin hybrid transgenes

Blood ◽  
2000 ◽  
Vol 95 (10) ◽  
pp. 3242-3249 ◽  
Author(s):  
Joel E. Rubin ◽  
Peter Pasceri ◽  
Xiumei Wu ◽  
Philippe Leboulch ◽  
James Ellis
Keyword(s):  
Gene Therapy ◽  
Transgenic Mice ◽  
Control Region ◽  
Globin Gene ◽  
Single Copy ◽  
Gene Sequences ◽  
Coding Sequences ◽  
Globin Promoter ◽  
Intron 2 ◽  
Chromatin Opening

Abstract The human β-globin locus control region (LCR) contains chromatin opening and transcriptional enhancement activities that are important to include in β-globin gene therapy vectors. We previously used single-copy transgenic mice to map chromatin opening activity to the 5′HS3 LCR element. Here, we test novel hybrid globin genes to identify β-globin gene sequences that functionally interact with 5′HS3. First, we show that an 850-base pair (bp) 5′HS3 element activates high-level β-globin gene expression in fetal livers of 17 of 17 transgenic mice, including 3 single-copy animals, but fails to reproducibly activate Aγ-globin transgenes. To identify the β-globin gene sequences required for LCR activity by 5′HS3, we linked the 815-bp β-globin promoter to Aγ-globin coding sequences (BGT34), together with either the β-globin intron 2 (BGT35), the β-globin 3′ enhancer (BGT54), or both intron 2 and the 3′ enhancer (BGT50). Of these transgenes, only BGT50 reproducibly expresses Aγ-globin RNA (including 7 of 7 single-copy animals, averaging 71% per copy). Modifications to BGT50 show that LCR activity is detected after replacing the β-globin promoter with the 700-bp Aγ-globin promoter, but is abrogated when an AT-rich region is deleted from β-globin intron 2. We conclude that LCR activity by 5′HS3 on globin promoters requires the simultaneous presence of β-globin intron 2 sequences and the 260-bp 3′ β-globin enhancer. The BGT50 construct extends the utility of the 5′HS3 element to include erythroid expression of nonadult β-globin coding sequences in transgenic animals and its ability to express antisickling γ-globin coding sequences at single copy are ideal characteristics for a gene therapy cassette.

Molecular Phylogeny of Ateline New World Monkeys (Platyrrhini, Atelinae) Based on γ-Globin Gene Sequences: Evidence ThatBrachytelesIs the Sister Group ofLagothrix

1999 ◽  
Vol 12 (1) ◽  
pp. 10-30 ◽  
Author(s):  
Carla M. Meireles ◽  
John Czelusniak ◽  
Maria P.C. Schneider ◽  
Jose A.P.C. Muniz ◽  
Maria C. Brigido ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
New World ◽  
Globin Gene ◽  
Sister Group ◽  
New World Monkeys ◽  
Gene Sequences

Purification of large native DNA fragments enriched in globin gene sequences

Gene ◽  
1978 ◽  
Vol 3 (1) ◽  
pp. 81-85 ◽  
Author(s):  
A.P. Ryskov ◽  
N.E. Maleeva ◽  
S.A. Limborska
Keyword(s):  
Globin Gene ◽  
Dna Fragments ◽  
Gene Sequences

Heterogeneity of the γ-Globin Gene Sequences in Japanese Individuals: Implication of Gene Conversion in Generation of Polymorphisms1

1989 ◽  
Vol 105 (2) ◽  
pp. 184-189 ◽  
Author(s):  
Satoshi Shiokawa ◽  
Supan Fucharoen ◽  
Goonnapa Fucharoen ◽  
Shunji Tomatsu ◽  
Yasuyuki Fukumaki
Keyword(s):  
Gene Conversion ◽  
Globin Gene ◽  
Gene Sequences

Cloning and amplification of α and β mouse globin gene sequences synthesised in vitro

Gene ◽  
1977 ◽  
Vol 1 (3-4) ◽  
pp. 229-239 ◽  
Author(s):  
Francois Rougeon ◽  
Bernard Mach
Keyword(s):  
Globin Gene ◽  
Gene Sequences

Structure of recombinant plasmids containing synthetic human foetal globin gene sequences

1978 ◽  
Vol 165 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Peter Humphries ◽  
Lesley W. Coggins ◽  
Robert W. Old ◽  
G. Joan Mitchell ◽  
Chris Coleclough ◽  
...  
Keyword(s):  
Globin Gene ◽  
Gene Sequences ◽  
Recombinant Plasmids
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