The portable dictionary of the mouse genome: a personal database for gene mapping and molecular biology

1994 ◽  
Vol 5 (6) ◽  
pp. 372-375 ◽  
Author(s):  
R. W. Williams
Keyword(s):  
Molecular Biology ◽  
Gene Mapping ◽  
Mouse Genome

Genetic applications of transposable elements in eukaryotes

Genome ◽  
1994 ◽  
Vol 37 (4) ◽  
pp. 519-525 ◽  
Author(s):  
Chaoqiang Lai
Keyword(s):  
Gene Transfer ◽  
Molecular Biology ◽  
Transposable Elements ◽  
Gene Mapping ◽  
Transposable Element ◽  
Gene Cloning ◽  
Insertional Mutagenesis ◽  
Genetic Research ◽  
Potential Applications ◽  
Mapping Gene

Transposable elements have many potential applications in genetic research, including insertional mutagenesis, gene mapping, gene cloning, gene transfer within and between species, and identification of genes expressed in specific tissues at a particular time. All these genetic approaches are important in the study of molecular biology and evolution. As the number of known transposable element families increases and their properties are further documented, their utility as genetic research tools will become greater. The purpose of this article is to discuss the salient properties of transposable elements in eukaryotes and their applications to genetic research.Key words: transposable elements, mutagenesis, plants, Drosophila, genetic application.

scholarly journals Identification and applications of repetitive probes for gene mapping in the mouse.

Genetics ◽  
1991 ◽  
Vol 127 (1) ◽  
pp. 169-179
Author(s):  
L D Siracusa ◽  
N A Jenkins ◽  
N G Copeland
Keyword(s):  
Genetic Variation ◽  
Gene Mapping ◽  
Mouse Genome ◽  
Interspecific Cross ◽  
Single Copy ◽  
Molecular Probes ◽  
Envelope Gene ◽  
Neoplastic Disease ◽  
Virus Envelope ◽  
Tumor Virus

Abstract Interspecific mouse hybrids that are viable and fertile provide a wealth of genetic variation that is useful for gene mapping. We are using this genetic variation to develop multilocus linkage maps of the mouse genome. As an outgrowth of this work, we have identified three repetitive probes that collectively identify 28 loci dispersed on 16 of the 19 mouse autosomes and the X chromosome. These loci establish a skeleton linkage map that can be used to detect linkage over much of the mouse genome. The molecular probes are derived from the mouse mammary tumor virus envelope gene, the ornithine decarboxylase gene, and the triose phosphate isomerase gene. The ability to scan the mouse genome quickly and efficiently in an interspecific cross using these three repetitive probes makes this system a powerful tool for identifying the chromosomal location of mutations that have yet to be cloned, mapping multigenic traits, and identifying recessive protooncogene loci associated with murine neoplastic disease. Ultimately, interspecific hybrids in conjunction with repetitive and single-copy probes will provide a rapid means to access virtually any gene of interest in the mouse genome at the molecular level.

Introduction

1978 ◽  
Vol 36 (3) ◽  
pp. 543-544
Author(s):  
W. Bernard
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Nucleic Acid ◽  
Gene Mapping ◽  
Molecular Genetics ◽  
Cell Biology ◽  
Gene Activity ◽  
Close Connection ◽  
Basic Information ◽  
Simple Systems

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.

Electron Microscopy in Molecular Biology

1967 ◽  
Vol 25 ◽  
pp. 2-3
Author(s):  
Cecil E. Hall
Keyword(s):  
Electron Microscopy ◽  
Molecular Biology ◽  
Noise Level ◽  
Molecular Structures ◽  
Material Structure ◽  
The Arts ◽  
Shadow Casting ◽  
Electron Image ◽  
High Degree ◽  
Very High

The visualization of organic macromolecules such as proteins, nucleic acids, viruses and virus components has reached its high degree of effectiveness owing to refinements and reliability of instruments and to the invention of methods for enhancing the structure of these materials within the electron image. The latter techniques have been most important because what can be seen depends upon the molecular and atomic character of the object as modified which is rarely evident in the pristine material. Structure may thus be displayed by the arts of positive and negative staining, shadow casting, replication and other techniques. Enhancement of contrast, which delineates bounds of isolated macromolecules has been effected progressively over the years as illustrated in Figs. 1, 2, 3 and 4 by these methods. We now look to the future wondering what other visions are waiting to be seen. The instrument designers will need to exact from the arts of fabrication the performance that theory has prescribed as well as methods for phase and interference contrast with explorations of the potentialities of very high and very low voltages. Chemistry must play an increasingly important part in future progress by providing specific stain molecules of high visibility, substrates of vanishing “noise” level and means for preservation of molecular structures that usually exist in a solvated condition.

An introduction to the themed issue on RNA biology in China

2020 ◽  
Vol 64 (6) ◽  
pp. 863-866
Author(s):  
Zhe Wu
Keyword(s):  
Molecular Biology ◽  
Chinese Society ◽  
Fortieth Anniversary ◽  
Rna Biology ◽  
Research And Education ◽  
Education In China

Abstract The year 2019 marked the fortieth anniversary of the Chinese Society of Biochemistry and Molecular Biology (CSBMB), whose mission is to promote biomolecular research and education in China. The last 40 years have witnessed tremendous growth and achievements in biomolecular research by Chinese scientists and Essays in Biochemistry is delighted to publish this themed issue that focuses on exciting areas within RNA biology, with each review contributed by key experts from China.

Sign in / Sign up

Export Citation Format

Share Document